2012 Fiscal Year Final Research Report
Three-dimensional ultrastructural study of Endothelial cells and pericytes interdigitation in angiogenesis
Project/Area Number |
21580371
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Basic veterinary science/Basic zootechnical science
|
Research Institution | Azabu University |
Principal Investigator |
WAKUI Shin 麻布大学, 獣医学部, 准教授 (40201157)
|
Project Period (FY) |
2009 – 2012
|
Keywords | 病理 / 血管新生制御 |
Research Abstract |
Endothelial cells and pericytes play critical role in angiogenesis by the angiopoietin system. Endothelial cell and pericyte interdigitations (EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. Rats subcutaneous implantation of a thermoreversible gelation polymer discs were inqubated in vitro 24-72hr. The capillary density increased to a peak on 48hr. A number of EPI were observed to a peak on 72hr. Serial sectioning electron microscopy immunohistochemical analysis revealed that Angiopoietin-1 localized at Pericytes and EPI. Quantitative RT-PCR analysis revealed that the level of Angiopoietin-1 increased on 72hr. The present study showed that the microvesselmaturation might to be involved in the expression of EPI and Angiopoietin-1.
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Research Products
(21 results)
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[Journal Article] Atypical Leydig cell hyperplasia in adult rats with low T and high LH induced by prenatal di(n-butyl) phthalate exposure.2013
Author(s)
Wakui, S., Takahashi, H., Muto, T., Shirai,M., Jutabha, P., Anzai,N., Wempe, M.F. Kansaku, N., Hano, H., Inomata,T. and Endou,H.
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Journal Title
Toxicol. Pathol
Volume: 41
Pages: 480-486
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[Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis2012
Author(s)
Wakui, S., Mtohashi, M., Imai,K.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Pathol
Volume: 25
Pages: M15
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[Journal Article] Sertoli cellsproliferate in adult rats with prenatalexposure to 3,3',4,4',5-pentachlorobiphenyl2012
Author(s)
Wakui, S., Muto, T., Suzuki, Y., Takahashi, H. and Hano, H
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Journal Title
Arch. Toxicol
Volume: 86
Pages: 159-162
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[Journal Article] Metabolism and pharmacokineticstudies of JPH203, an L-amino acidtransporter2012
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W.,Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H
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Journal Title
Drug Metab. Pharmacokineti
Volume: 27
Pages: 155-161
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[Journal Article] Potent hURAT1 inhibitors: in vitro andin vivo metabolism and pharmacokinetic studies2012
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W.,Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H
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Journal Title
Drug Design, Development and Therapy
Volume: 6
Pages: 323-339
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[Journal Article] Testicularspermiation failure in rats exposed prenatally to 3,3′,4,4′,5- pentachlorobiphenyl2011
Author(s)
Wakui, S., Muto, T., Motohashi, M.,Kobayashi, Y., Suzuki, Y., Takahashi, H. and Hano
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Journal Title
J. Toxicol. Sci
Volume: 35
Pages: 757-765
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[Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis.2011
Author(s)
Wakui, S., Imai, K., Mtohashi, M.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Pathol
Volume: 24
Pages: M64
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[Journal Article] MicroPNAchanges induced by heterocyclic amines and its significance in the early satges of colon charcinogenesis2011
Author(s)
Imai, K., Ochiai, M., Hippo, Y., Igarashi,M., Wakui, S. and Nakagama, H
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Journal Title
J. Toxicol. Pathol
Volume: 24
Pages: M60
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[Journal Article] Sex-associated difference in estrogen receptor βexpression in N-methyl-N′-nitro-N- nitrosoguanidine-induced rat gastric cancers2011
Author(s)
Wakui, S., Motohashi, M., Muto, T., Takahashi, H., Hano, H., Jutabha, P., Anzai,N., Wempe, M.F. and Endou, H
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Journal Title
Comparative. Med
Volume: 61
Pages: 1-7
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[Journal Article] Cyclin D1/cdk4, estrogen receptors α and β, inN-methyl-N'-nitro-N-nitrosoguanidine- induced rat gastric carcinogenesis: immunohistochemical study2011
Author(s)
Motohashi, M., Wakui, S., Muto, T.,Suzuki, Y., Shirai, M., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Sci
Volume: 36
Pages: 373-378
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[Journal Article] In vitrothermoreversible gel disc quantitative assay of rat angiogenesis2011
Author(s)
Wakui, S., Muto, T., Anzai, N., Takahashi, H., Jutabha, P., Sakata, A., Wempe, M.F.,Hano, H. and Endou, H.
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Journal Title
AATEX
Volume: 16
Pages: 59-65
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[Journal Article] Human uric acid transporter1: an inhibitor structure-activity relationship study2011
Author(s)
Wempe, M.F., Quade, B., Jutabha, P., Iwen, TJ., Frick, M.F., Rice, PJ., Wakui, S.and Endou, H
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Journal Title
Nucleosides Nucleotides Nucleic Acids
Volume: 30
Pages: 1312-1323
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[Journal Article] Human drug transporter gene-expressing cells are useful alternatives to predictpharmacokinetics in man2011
Author(s)
Anzai, N., Wakui, S., Jutabha, P., Muto, T., Hayashi, M., Hayashi, K., Domae, M.,Uchida, K., Wempe, M.F. and Endou, H
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Journal Title
AATEX
Volume: 16
Pages: 66-73
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[Journal Article] Apical voltage-driven urate effiluxtransporter NPT4 in renal proximal tubule2011
Author(s)
Jutabha, P., Anzai, N., Wempe, M.F., Wakui, S., Endou, H. and Sakurai, H
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Journal Title
Nucleosides Nucleotides Nucleic Acids
Volume: 30
Pages: 1302-1311
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[Journal Article] Potent hURAT1 inhibitors: in vitro and in vivo metabolism and pharmacokineticstudies2011
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W., Jutabha, P., Hayashi, M., Anzai, N., Wakui,S., Kusuhara, H., Sugiyama, Y. and Endou,H
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Journal Title
Drug Design, Develop, Therapy
Volume: 30
Pages: 3112-3113
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[Journal Article] Testicular spermiation failure in rats exposed prenatally to 3,3',4,4',5- pentachlorobiphenyl2010
Author(s)
Wakui, S., Muto, T., Motohashi, M.,Kobayashi, Y., Suzuki, Y., Shirai, M., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Sci
Volume: 35
Pages: 757-765
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[Journal Article] LAT1 antagonists suppressed N-methyl-N'- nitrosoguanidine induced rat gastric carcinogenesis2010
Author(s)
Ishida, K., Muto, T., Motohashi, M.,Kobayashi, Y., Imai, K. and Wakui, S
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Journal Title
J. Toxicol. Pathol
Volume: 23
Pages: M43
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[Journal Article] Male-female rats gastric carcinogenesis induced by N-methy-N'-nitrosoguanidine phthalate2010
Author(s)
Motohashi, M., Muto, T., Ishida, K.,Kobayashi, Y., Imai, K. and Wakui, S
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Journal Title
J. Toxicol. Pathol
Volume: 23
Pages: M56
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[Journal Article] Lung and testicular development in ratswith prenatal perfluorooctane-sulfate exposure2010
Author(s)
Kobayashi, Y., Muto, T., Ishida, K., Motohashi, M., Imai, K. and Wakui, S
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Journal Title
J. Toxicol. Pathol.
Volume: 23
Pages: M56
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[Journal Article] Rats prenatally3,3',4,4',5-Pentachlorobiphenyl (PCB126)Modulate ERK and ERαexpression inN-nitrobis(2-hydroxypropyl)amine (BHP)induced lung carcinogenesis2009
Author(s)
Kobayashi, Y., Ishida, K., Motohashi, M.,Muto, T. and Wakui, S
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Journal Title
J. Toxicol. Pathol
Volume: 22
Pages: M18