Three-dimensional ultrastructural study of Endothelial cells and pericytes interdigitation in angiogenesis

2012 Fiscal Year Final Research Report

• Project/Area Number: 21580371
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Azabu University
• Principal Investigator: WAKUI Shin 麻布大学, 獣医学部, 准教授 (40201157)
• Project Period (FY): 2009 – 2012
• Keywords: 病理 / 血管新生制御

Research Abstract

Endothelial cells and pericytes play critical role in angiogenesis by the angiopoietin system. Endothelial cell and pericyte interdigitations (EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. Rats subcutaneous implantation of a thermoreversible gelation polymer discs were inqubated in vitro 24-72hr. The capillary density increased to a peak on 48hr. A number of EPI were observed to a peak on 72hr. Serial sectioning electron microscopy immunohistochemical analysis revealed that Angiopoietin-1 localized at Pericytes and EPI. Quantitative RT-PCR analysis revealed that the level of Angiopoietin-1 increased on 72hr. The present study showed that the microvesselmaturation might to be involved in the expression of EPI and Angiopoietin-1.

Research Products

• [Journal Article] Atypical Leydig cell hyperplasia in adult rats with low T and high LH induced by prenatal di(n-butyl) phthalate exposure. (2013)
  • Author(s): Wakui, S., Takahashi, H., Muto, T., Shirai,M., Jutabha, P., Anzai,N., Wempe, M.F. Kansaku, N., Hano, H., Inomata,T. and Endou,H.
  • Journal Title: Toxicol. Pathol Volume: 41 Pages: 480-486
• [Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis (2012)
  • Author(s): Wakui, S., Mtohashi, M., Imai,K.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T., Takahashi, H. and Hano, H
  • Journal Title: J. Toxicol. Pathol Volume: 25 Pages: M15
• [Journal Article] Sertoli cellsproliferate in adult rats with prenatalexposure to 3,3',4,4',5-pentachlorobiphenyl (2012)
  • Author(s): Wakui, S., Muto, T., Suzuki, Y., Takahashi, H. and Hano, H
  • Journal Title: Arch. Toxicol Volume: 86 Pages: 159-162
• [Journal Article] Metabolism and pharmacokineticstudies of JPH203, an L-amino acidtransporter (2012)
  • Author(s): Wempe, M.F., Rice, P.J., Lightner, J.W.,Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H
  • Journal Title: Drug Metab. Pharmacokineti Volume: 27 Pages: 155-161
• [Journal Article] Potent hURAT1 inhibitors: in vitro andin vivo metabolism and pharmacokinetic studies (2012)
  • Author(s): Wempe, M.F., Rice, P.J., Lightner, J.W.,Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H
  • Journal Title: Drug Design, Development and Therapy Volume: 6 Pages: 323-339
• [Journal Article] Testicularspermiation failure in rats exposed prenatally to 3,3′,4,4′,5- pentachlorobiphenyl (2011)
  • Author(s): Wakui, S., Muto, T., Motohashi, M.,Kobayashi, Y., Suzuki, Y., Takahashi, H. and Hano
  • Journal Title: J. Toxicol. Sci Volume: 35 Pages: 757-765
• [Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis. (2011)
  • Author(s): Wakui, S., Imai, K., Mtohashi, M.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T., Takahashi, H. and Hano, H
  • Journal Title: J. Toxicol. Pathol Volume: 24 Pages: M64
• [Journal Article] MicroPNAchanges induced by heterocyclic amines and its significance in the early satges of colon charcinogenesis (2011)
  • Author(s): Imai, K., Ochiai, M., Hippo, Y., Igarashi,M., Wakui, S. and Nakagama, H
  • Journal Title: J. Toxicol. Pathol Volume: 24 Pages: M60
• [Journal Article] Sex-associated difference in estrogen receptor βexpression in N-methyl-N′-nitro-N- nitrosoguanidine-induced rat gastric cancers (2011)
  • Author(s): Wakui, S., Motohashi, M., Muto, T., Takahashi, H., Hano, H., Jutabha, P., Anzai,N., Wempe, M.F. and Endou, H
  • Journal Title: Comparative. Med Volume: 61 Pages: 1-7
• [Journal Article] Cyclin D1/cdk4, estrogen receptors α and β, inN-methyl-N'-nitro-N-nitrosoguanidine- induced rat gastric carcinogenesis: immunohistochemical study (2011)
  • Author(s): Motohashi, M., Wakui, S., Muto, T.,Suzuki, Y., Shirai, M., Takahashi, H. and Hano, H
  • Journal Title: J. Toxicol. Sci Volume: 36 Pages: 373-378
• [Journal Article] In vitrothermoreversible gel disc quantitative assay of rat angiogenesis (2011)
  • Author(s): Wakui, S., Muto, T., Anzai, N., Takahashi, H., Jutabha, P., Sakata, A., Wempe, M.F.,Hano, H. and Endou, H.
  • Journal Title: AATEX Volume: 16 Pages: 59-65
• [Journal Article] Human uric acid transporter1: an inhibitor structure-activity relationship study (2011)
  • Author(s): Wempe, M.F., Quade, B., Jutabha, P., Iwen, TJ., Frick, M.F., Rice, PJ., Wakui, S.and Endou, H
  • Journal Title: Nucleosides Nucleotides Nucleic Acids Volume: 30 Pages: 1312-1323
• [Journal Article] Human drug transporter gene-expressing cells are useful alternatives to predictpharmacokinetics in man (2011)
  • Author(s): Anzai, N., Wakui, S., Jutabha, P., Muto, T., Hayashi, M., Hayashi, K., Domae, M.,Uchida, K., Wempe, M.F. and Endou, H
  • Journal Title: AATEX Volume: 16 Pages: 66-73
• [Journal Article] Apical voltage-driven urate effiluxtransporter NPT4 in renal proximal tubule (2011)
  • Author(s): Jutabha, P., Anzai, N., Wempe, M.F., Wakui, S., Endou, H. and Sakurai, H
  • Journal Title: Nucleosides Nucleotides Nucleic Acids Volume: 30 Pages: 1302-1311
• [Journal Article] Potent hURAT1 inhibitors: in vitro and in vivo metabolism and pharmacokineticstudies (2011)
  • Author(s): Wempe, M.F., Rice, P.J., Lightner, J.W., Jutabha, P., Hayashi, M., Anzai, N., Wakui,S., Kusuhara, H., Sugiyama, Y. and Endou,H
  • Journal Title: Drug Design, Develop, Therapy Volume: 30 Pages: 3112-3113
• [Journal Article] Testicular spermiation failure in rats exposed prenatally to 3,3',4,4',5- pentachlorobiphenyl (2010)
  • Author(s): Wakui, S., Muto, T., Motohashi, M.,Kobayashi, Y., Suzuki, Y., Shirai, M., Takahashi, H. and Hano, H
  • Journal Title: J. Toxicol. Sci Volume: 35 Pages: 757-765
• [Journal Article] LAT1 antagonists suppressed N-methyl-N'- nitrosoguanidine induced rat gastric carcinogenesis (2010)
  • Author(s): Ishida, K., Muto, T., Motohashi, M.,Kobayashi, Y., Imai, K. and Wakui, S
  • Journal Title: J. Toxicol. Pathol Volume: 23 Pages: M43
• [Journal Article] Male-female rats gastric carcinogenesis induced by N-methy-N'-nitrosoguanidine phthalate (2010)
  • Author(s): Motohashi, M., Muto, T., Ishida, K.,Kobayashi, Y., Imai, K. and Wakui, S
  • Journal Title: J. Toxicol. Pathol Volume: 23 Pages: M56
• [Journal Article] Lung and testicular development in ratswith prenatal perfluorooctane-sulfate exposure (2010)
  • Author(s): Kobayashi, Y., Muto, T., Ishida, K., Motohashi, M., Imai, K. and Wakui, S
  • Journal Title: J. Toxicol. Pathol. Volume: 23 Pages: M56
• [Journal Article] In a Sprague-Dawley rat: Sertori-Leidig cell tumor of the testis (2009)
  • Author(s): Wakui, S., Kobayashi, Y., Ishida, K., Motohashi, M. and Muto, T
  • Journal Title: J. Toxicol. Pathol Volume: 22 Pages: M55
• [Journal Article] Rats prenatally3,3',4,4',5-Pentachlorobiphenyl (PCB126)Modulate ERK and ERαexpression inN-nitrobis(2-hydroxypropyl)amine (BHP)induced lung carcinogenesis (2009)
  • Author(s): Kobayashi, Y., Ishida, K., Motohashi, M.,Muto, T. and Wakui, S
  • Journal Title: J. Toxicol. Pathol Volume: 22 Pages: M18