Development of an effective treatment for the reduction of Campylobacter jejuni on chicken carcasses by resonant ultrasonication and microbubbles

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: University of Miyazaki
• Principal Investigator: MISAWA Naoaki 宮崎大学, 農学部, 教授 (20229678)
• Project Period (FY): 2009 – 2011
• Keywords: 食品 / 細菌 / 獣医学 / マイクロバブル / 超音波

Research Abstract

Healthy chickens are considered as reservoirs for campylobacters and the organisms colonize in the intestinal tract. Contamination of chicken carcasses with C. jejuni and C. coli occurs over the entire skin surface during processing. In this study, an effective treatment for decontaminating campylobacters using naturally contaminated chicken carcasses was developed. The present study showed that combinations of decontamination techniques of chicken carcasses immerse in cetylpiridinium chloride, vacuum, and ultrasonic generator for water resonance, were highly effective to reduce campylobacters on the skins of carcasses with naturally contaminated chickens.

Research Products

• [Journal Article] Correlations between 23S rRNA genes and erythromycin resistance in Campylobacter jejuni. (2012)
  • Author(s): Tazumi, A., Xu, J., Han, L., Misawa, N., Moore, J., Millar, C., and Matsuda, M
  • Journal Title: Ann. Microbiol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Uneven distribution of the luxS gene within the genus Campylobacter (2011)
  • Author(s): Tazumi, A., Negoro, M., Tomiyama, Y., Misawa, N., Itoh, K., Moore, J. E., Millar, B. C., Matsuda, M
  • Journal Title: Br. J. Biomed. Sci Volume: 68 Pages: 19-22
  • Peer Reviewed
• [Journal Article] Qfever in Japan : An update review (2011)
  • Author(s): Porter, S. R., Czaplicki, G., Mainil, J., Horii, Y., Misawa, N, and Saegerman, C
  • Journal Title: Vet. Microbiol Volume: 149 Pages: 198-306
  • Peer Reviewed
• [Journal Article] 超音波を使用したカンピロバクター撃退技術 (2010)
  • Author(s): 三澤尚明、副島潤一郎
  • Journal Title: 超音波TECHNO Volume: 22 Pages: 6-9
• [Journal Article] The mode of biofilm formation on smooth surfaces by Campylobacter jejuni (2010)
  • Author(s): Moe, K. K., Mimura, J., Ohnishi, T., Wake, T., Yamazaki, W., Masaaki Nakai, M., and Misawa, N
  • Journal Title: J. Vet. Med. Sci Volume: 72 Pages: 411-416
  • Peer Reviewed
• [Journal Article] Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes in Vibrio parahaemolyticus and related Vibrio species (2009)
  • Author(s): Yamazaki, W., Kumeda, Y., Misawa, N., Nakaguchi, Y., and Nishibuchi, M
  • Journal Title: Appl. Environ. Microbiol Volume: 76 Pages: 820-828
  • Peer Reviewed
• [Journal Article] Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters (2009)
  • Author(s): Tazumi, A., Kakinuma, Y., Misawa, N., Moore, J., Millar, J. B. C., and Matsuda, M
  • Journal Title: BMC Microbiology Volume: 9 Pages: 256
  • DOI: doi:10.1186/1471-2180-9-256
  • Peer Reviewed
• [Journal Article] Absence of intervening sequences(IVSs) in helix 11 region within 16S rRNA genes among more than 240 isolates of the seven Campylobacter species (2009)
  • Author(s): Sekizuka, A., Tazumi, A., Nakanishi, S., Meguro, S., Kakinuma, Y., Misawa, N., Moore, J. E., Millar, B. C., Matsuda, M
  • Journal Title: Br. J. Biomed. Sci Volume: 66 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Comparison of Campylobacter jejuni and Campylobacter coli detection in naturally contaminated chicken meat samples between loop-mediated isothermal amplification assay and conventional culture methods (2009)
  • Author(s): Yamazaki, W., Taguchi, M., Kawai, T., Kawatsu, K., Sakata, J., Inoue, K., Misawa, N
  • Journal Title: Appl. Environ. Microbiol Volume: 75 Pages: 1597-1603
  • Peer Reviewed
• [Presentation] 食鳥と体皮膚の各処理工程におけるカンピロバクターの定量検査と冷却前後の羽毛包の観察 (2011)
  • Author(s): 浦田彩香、新木泰輔、佐々木賢美、谷口喬子、山崎渉、三澤尚明
  • Organizer: 日本獣医公衆衛生学会(九州)
  • Place of Presentation: 長崎市
  • Year and Date: 2011-10-30
• [Presentation] 食鳥処理工程におけるブロイラーと体皮膚へのCampylobacter jejuniの付着能について (2011)
  • Author(s): 大城昇太郎、新木泰輔、谷口喬子、山崎渉、三澤尚明
  • Organizer: 第152回日本獣医学科学術集会
  • Place of Presentation: 大阪府
  • Year and Date: 2011-09-20
• [Presentation] Development of an effective decontamination of Campylobacter species on chicken carcasses (2011)
  • Author(s): Misawa, N., Shinki, T., Sasaki, S., Taniguchi, T., Yamazaki, W
  • Organizer: 16th International Campylobacter, Helicobacter, and related organisms, Vancouver
  • Place of Presentation: Canada
  • Year and Date: 2011-08-30
• [Presentation] 新しい概念に基づく食鳥と体のカンピロバクター制御法の開発 (2010)
  • Author(s): 三澤尚明
  • Organizer: 第3回日本カンピロバクター研究会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-12-03
• [Presentation] オゾン含有マイクロバブル水によるマンゴーの鮮度保持並びに殺菌効果 (2010)
  • Author(s): 三澤尚明、佐々木賢美、吉山佳世、福井敬一
  • Organizer: 日本食品微生物学会
  • Place of Presentation: 大津市
  • Year and Date: 2010-11-11
• [Presentation] 食鳥肉における新しいカンピロバクター制御技術の開発 (2010)
  • Author(s): 新木泰輔、佐々木賢美、山崎渉、三澤尚明
  • Organizer: 日本食品微生物学会
  • Place of Presentation: 大津市
  • Year and Date: 2010-11-11
• [Presentation] 血清中に含まれるCampylobacter jejuniの自発凝集活性促進物質の検出 (2010)
  • Author(s): 吉本有貴、山崎渉、三澤尚明
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 帯広市
  • Year and Date: 2010-09-16
• [Presentation] 鶏と体のカンピロバクター制御技術の開発 (2010)
  • Author(s): 三澤尚明、佐々木賢美、山崎渉
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広市
  • Year and Date: 2010-09-16
• [Presentation] 食肉の衛生管理について-食鳥肉におけるカンピロバクターの制御を中心に- (2010)
  • Author(s): 三澤尚明
  • Organizer: 大分県公衆衛生獣医師協議会研修会
  • Place of Presentation: 大分市
  • Year and Date: 2010-06-01
• [Book] 動物の感染症第三版 (2011)
  • Author(s): 三澤尚明
  • Total Pages: 131
  • Publisher: 近代出版(東京)
• [Book] 獣医微生物学第3版 (2011)
  • Author(s): 三澤尚明
  • Total Pages: 94-97
  • Publisher: 文永堂出版(東京)
• [Book] 新・食肉衛生検査マニュアル全国食肉衛生検査所協議会編 (2011)
  • Author(s): 三澤尚明
  • Total Pages: 202-205
  • Publisher: 中央法規出版(東京)
• [Book] 動物の感染症第3版 (2009)
  • Author(s): 三澤尚明
  • Total Pages: 131
  • Publisher: 近代出版(東京)
• [Remarks]
  • URL: http://www.agr.miyazaki-u.ac.jp/～vet/Vet-publichel2/index.htm
• [Patent(Industrial Property Rights)] 真空及び共振型超音波処理による食品材料における微生物の制御方法及び制御装置 (2011)
  • Inventor(s): 三澤尚明、副島潤一郎、小山克宏
  • Industrial Property Rights Holder: 宮崎大学、株・カイジョー
  • Industrial Property Number: 特許、特願2012-507123
  • Filing Date: 2011-03-18
  • Overseas
• [Patent(Industrial Property Rights)] 口蹄疫ウイルスの包括的検出法 (2011)
  • Inventor(s): 山崎渉、三澤尚明
  • Industrial Property Rights Holder: 宮崎大学
  • Industrial Property Number: 特許、特願2012-003740
  • Filing Date: 2011-01-12