DYNAMISM OF GYCAN CHAIN RESPONSE IN ACUTE PHASE PROTEINS ON SEVERE DISEASE CONDITIONS AND MODIFICATION OF GYCAN CHAIN FUNCTION

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580392
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: IWATA Hiroyuki 山口大学, 農学部, 教授 (40193750)
• Co-Investigator(Kenkyū-buntansha): MAEDA Ken 山口大学, 農学部, 教授 (90284273) ; KATO Hirotomo 山口大学, 農学部, 准教授 (00346579)
• Project Period (FY): 2009 – 2011
• Keywords: 急性期蛋白 / α1酸性糖蛋白 / 糖鎖修飾 / 腫瘍 / ウイルス

Research Abstract

For the purpose of analyses on dynamism of glycan chain response in acute phase proteins under severe disease conditions, alpha-1 acid glycoprotein(AGP) in bovine, and feline were purified from sera and ascites, respectively, and recombinant AGP. were also purified. These proteins were used for the preparation of monoclonal antibodies(Mabs) for the analyses of AGPs. In results, several Mabs for each animal were obtained and analysed for the epitopes of each AGP, which were useful for quantification of AGPs and analyses of glycan chain.

Research Products

• [Journal Article] Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic (2012)
  • Author(s): Fujita M, Kato H, Caceres AG, Gomez EA, Velez L, Mimori T, Zhang F, Iwata H, Korenaga M, Sakurai T, Katakura K, Hashiguchi Y
  • Journal Title: Acta Tropica Volume: 121 Pages: 93-98
  • Peer Reviewed
• [Journal Article] Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1(PSGL-1) (2011)
  • Author(s): Umeki S, Suzuki R, Shimojima M, Ema Y, Yanase T, Iwata H, Okuda M, Mizuno T
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 142 Pages: 119-125
  • Peer Reviewed
• [Journal Article] Natural infections of man-biting sand flies by Leishmania and Trypanosoma species in the northern Peruvian Andes (2011)
  • Author(s): Kato H, Gomez EA, Caceres AG, Vargas F, Mimori T, Yamamoto K, Iwata H, Korenaga M, Velez L, Hashiguchi Y
  • Journal Title: Vector Borne Zoonotic Disease Volume: 11 Pages: 515-521
  • Peer Reviewed
• [Journal Article] Use of FTA cards for direct sampling of patients' lesions in the ecological study of cutaneous leishmaniasis (2010)
  • Author(s): Kato H, Caceres AG, Mimori T, Ishimaru Y, Sayed AS, Fujita M, Iwata H, Uezato H, Velez LN, Gomez EA, Hashiguchi Y
  • Journal Title: Journal of Clinical Microbiology Volume: 48 Pages: 3661-3665
  • Peer Reviewed
• [Journal Article] Dogs as sentinels for human infection with Japanese encephalitis virus (2010)
  • Author(s): Shimoda H, Ohno Y, Mochizuki M, Iwata H, Okuda M, Maeda K
  • Journal Title: Emerging Infectious Disease Volume: 16 Pages: 1137-1139
  • Peer Reviewed
• [Journal Article] Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan (2010)
  • Author(s): Kato H, Uezato H, Sato H, Bhutto AM, Soomro FR, Baloch JH, Iwata H, Hashiguchi Y
  • Journal Title: Parasite Vectors Pages: 3-10
  • Peer Reviewed
• [Journal Article] A repertoire of the dominant transcripts from the salivary glands of the blood-sucking bug, Triatoma dimidiata, a vector of Chagas disease, Infection (2010)
  • Author(s): Kato H, Jochim RC, Sakoda R, Iwata H, Gomez EA, Valenzuela JG, Hashiguchi Y
  • Journal Title: Genetics and Evolution Volume: 10 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Genetic diversity of ribosomal RNA internal transcribed spacer sequences in Lutzomyia species from areas endemic for New World cutaneous leishmaniasis (2009)
  • Author(s): Kuwahara K, Kato H, Gomez EA, Uezato H, Mimori T, Yamamoto Y, Calvopina M, Caceres AG, Iwata H, Hashiguchi Y
  • Journal Title: Acta Tropica Volume: 112 Pages: 131-136
  • Peer Reviewed
• [Journal Article] Adaptation of wild-type measles virus to cotton rat lung cells : E89K mutation in matrix protein contributes to its fitness (2009)
  • Author(s): Dong JB, Saito A, Mine Y, Sakuraba Y, Nibe K, Goto Y, Komase K, Nakayama T, Miyata H, Iwata H, Haga T
  • Journal Title: Virus Genes Volume: 39 Pages: 330-334
  • Peer Reviewed
• [Journal Article] Nationwide survey of leptospira antibodies in dogs in Japan : results from microscopic agglutination test and enzyme-linked immunosorbent assay (2009)
  • Author(s): Iwamoto E, Wada Y, Fujisaki Y, Umeki S, Jones MY, Mizuno T, Itamoto K, Maeda K, Iwata H, Okuda M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 71 Pages: 1191-1199
  • Peer Reviewed
• [Journal Article] Functional characterization of a salivary apyrase from the sand fly (2009)
  • Author(s): Hamasaki R, Kato H, Terayama Y, Iwata H, Valenzuela JG
  • Journal Title: Phlebotomus duboscqi, a vector of Leishmania major Volume: 55 Pages: 1044-1049
  • Peer Reviewed
• [Presentation] Jesus Valenzuela,リーシュマニア原虫媒介サシチョウバエPhlebotomus duboscqiの唾液アピラーゼの機能解析 (2010)
  • Author(s): 加藤大智, 濱崎亮一, 寺山好美, 岩田祐之
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学,東京
  • Year and Date: 2010-03-28
• [Presentation] ペルーにおけるサシチョウバエの遺伝子タイピング法の確立 (2010)
  • Author(s): 藤田恵, 加藤大智, Abraham Caceres, 岩田祐之, Eduardo Gomez, 橋口義久
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学,東京
  • Year and Date: 2010-03-28
• [Presentation] シャーガス病媒介サシガメTriatoma dimidiate唾液中にみとめられたtriabin様タンパクの機能解析 (2010)
  • Author(s): 石丸由佳, 加藤大智, 藤田恵, 岩田祐之, Eduardo Gomez, 橋口義久
  • Place of Presentation: 日本獣医生命科学大学,東京
  • Year and Date: 2010-03-28