2011 Fiscal Year Final Research Report
Making virus tolerant plants by using Dicer and dsRNA-binding protein genes
| Project/Area Number |
21580411
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NATSUAKI Tomohide 宇都宮大学, 農学部, 教授 (10134264)
MORIYAMA Hiromitsu 東京農工大学, 大学院・農学研究院, 准教授 (20392673)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 細胞機能 / ウイルス / RNA干渉 / 2本鎖RNA / ダイサー |
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| Research Abstract |
Inoculation experiments with Turnip mosaic virus on Arabidopsis plants with over-expressing Dicer-like protein(DCL1-4) or dsRNA-binding protein(DRB1-5) genes were carried out. Infected plants with over-expressing DRB2, DRB3 or DRB4 tended to exhibit mild symptom. Furthermore, a biochemical procedure for evaluating the dicer activity(dsRNA-cleaving activity) is established by using crude extracts from Arabidopsis seedlings.
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