Search on the genome scale of a translation enha ncer functioning in rice

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KATO Ko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80283935)
• Project Period (FY): 2009 – 2011
• Keywords: 生物・生体工学 / 植物 / 発現制御 / ポリソーム / マイクロアレイ

Research Abstract

I searched for the translation enhancer functioned in the rice which was a monocotyledonous model plant on a genome scale and succeeded in isolating plural 5' UTR which had translation enhancer activity of the double in comparison with 5' UTR which had been already reported as a rice type translation enhancer. In addition, because these 5' UTR showed translation enhancer activity in other monocotyledonous plants, the use as the monocotyledonous type translation enhancer is expected.

Research Products

• [Journal Article] High level expression of transgenes by use of 5'-untranslated region of the Arabidopsis thaliana arabinogalactan-protein 21gene in dicotyledons (2012)
  • Author(s): Matsui, T., Matsuura, H., Sawada, K., Takita, E., Kinjo, S., Takenami, S., Ueda, K., Nishigaki, N., Yamasaki, S., Hata, K., Yamaguchi, M., Demura, T. and Kato, K
  • Journal Title: Plant Biotechnology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Short Period of Mannitol but not LiCl Stress Leads to Global Translational Repression in Plants (2010)
  • Author(s): Matsuura, H., Kiyotaka, U., Ishibashi, Y., Kubo, Y., Yamaguchi, M., Hirata, K., Demura, T. and Kato, K
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74(10) Pages: 2110-2112
  • Peer Reviewed
• [Journal Article] Genome-wide analyses of early translational responses to elevated temperature and high salinity in Arabidopsis thaliana (2010)
  • Author(s): Matsuura, H., Ishibashi, Y., Shinmyo, A., Kanaya S. and Kato, K
  • Journal Title: Plant Cell physiol. Volume: 51(3) Pages: 448-462
  • Peer Reviewed
• [Journal Article] Effect of the Sequence Context of the AUG Initiation Codon on the Rate of Translation in Dicotyledonous and Monocotyledonous Plant Cells (2010)
  • Author(s): Sugio, T., Matsuura, H., Matsui, T., Matsunaga, M., Nosho, T., Kanaya, S., Shinmyo, A. and Kato, K
  • Journal Title: J. Biosci. Bioeng. Volume: 109(2) Pages: 170-173
  • Peer Reviewed
• [Presentation] 単子葉イネからの翻訳エンハンサーの探索 (2012)
  • Author(s): 矢村寿啓
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] 単子葉型新規翻訳エンハンサーの探索 (2011)
  • Author(s): 矢村寿啓
  • Organizer: 2011年度日本農芸化学会関西・中部支部合同大会
  • Place of Presentation: 京都
  • Year and Date: 2011-10-02
• [Presentation] 単子葉で機能する翻訳エンハンサーのゲノムスケールでの探索 (2011)
  • Author(s): 矢村寿啓
  • Organizer: 第29回日本植物細胞分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2011-09-07
• [Presentation] イネを対象とした熱ストレスによる翻訳状態の変化のゲノムワイド解析 (2010)
  • Author(s): 上田清貴
  • Organizer: 第28回日本植物分子細胞生物学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-03
• [Book] 植物におけるタンパク質翻訳の効率化、バイオ医薬品製造の効率化と生産基材の開発(山口照英監修) (2012)
  • Author(s): 上田清貴、加藤晃
  • Total Pages: 125-130
  • Publisher: シーエムシー出版