2011 Fiscal Year Final Research Report
Functional analysis of free N-glycans involved in regulation of plant differentiation.
| Project/Area Number |
21580414
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
KIMURA Yoshinobu 岡山大学, 大学院・自然科学研究科, 教授 (70195387)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 植物タンパク質 / 遊離N-グリカン / ENGase(endo-β-N-acetyl glucosaminidase) / PNGase(Peptide : N-glycanase) / 遺伝子発現抑制 / トランスジェニック植物 / 糖鎖機能 |
|---|
| Research Abstract |
In developing plant tissues, free N-glycan that are enzymatically liberated from glycoproteins occur ubiquitously at micromolar level. As a part of study to elucidate the physiological function(s) of these free N-glycans involved in plant development, we constructed knock-out or knock-down plants, in which the expressions of ENGase or PNGase genes were suppressed. The structural features of free N-glycans expressed in those transgenic plants were clearly distinguishable from those expressed in wild type plants. Although significant difference in phenotype between the transgenic plants and wild type was not observed, a specific difference in the fruit-ripening process was observed.
|
-
-
-
-
-
[Journal Article] A New Lectin from the Tuberous Rhizome of Kaempferia rotunda : Isolation, Characterization, Antibacterial and Antiproliferative Activities2011
Author(s)
Kabir S. R., Hossen, M. A., Zubair, M. A., Alom, M. J., Islam, M. F., Hossain, M. A., Kimura, Y.
-
Journal Title
Protein Pept Lett.
Volume: 18
Pages: 1140-1149
-
[Journal Article] Purification and Characterization of a Ca^<2+>-dependent novel lectin from nymphaea nouchali tuber with antiproliferative activities2011
Author(s)
Kabir SR, Zubair MA, Nurujjaman M, Haque MA, Hasan I, Islam MF, Hossain MT, Hossain MA, Rakib MA, Alam MT, Shaha RK, Hossain MT, Kimura Y, Absar N.
-
Journal Title
Biosci Rep.
Volume: 31
Pages: 465-475
-
-
-
-
[Journal Article] Molecular characterization of plant acidicα-mannosidase, a member of glycosylhydrolase family 38, involved in the turnover of N-glycans during tomato fruit ripening2010
Author(s)
Hossain, M. A., Nakano, R., Nakamura, K., Hossain, M. T., and Kimura, Y.
-
Journal Title
J. Biochem.
Volume: 148
Pages: 603-616
-
-
[Journal Article] Molecular cloning and gene expression analysis of tomato endo-β-N-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose type free N-glycans during tomato fruit ripening2009
Author(s)
Nakamura, K., Inoue, M., Nakano, R., Hosoi, K., and Kimura, Y.
-
Journal Title
Biosci. Biotechnol. Biochem.
Volume: 73
Pages: 461-464
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
