2011 Fiscal Year Final Research Report
Development of Molecular Species-Specific Live-Single Cell MS with Affinity-modified Nanospray Tips.
| Project/Area Number |
21590041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
TSUYAMA Naohiro 広島大学, 大学院・医歯薬学総合研究科, 講師 (10335747)
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| Co-Investigator(Kenkyū-buntansha) |
MASUJIMA Tsutomu 広島大学, 大学院・医歯薬学総合研究科, 教授 (10136054)
MIZUNO Hajime 広島大学, 大学院・医歯薬学総合研究科, 助手 (30457288)
TAKANORI Harada 広島大学, 大学院・医歯薬学総合研究科, 研究員 (30350325)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 分析化学 / 質量分析 |
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| Research Abstract |
We established Live Single-cell Mass Spectrometry which enables to clarify exhaustive molecular content of single cell recovered by a metal-coated glass tip following direct mass spectrometric analysis. In this study, we aimed to increase sensitivity and kinds of molecules by modifying the inner surface of the glass tips. We made the tips with hydrophilic or hydrophobic treatment which allow selective trapping of polar compounds or lipophilic compounds respectively. We proved treated tips to trap standard compound as well as molecules contained in single cell. Thus, detection of molecules with specific physical property can be detected by Live Single-cell Mass Spectrometry using this tip.
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