Development and application of a chemiluminescent measurement method folic acid related compounds as an arteriosclerosis marker

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: NAKASHIMA Kenichiro 長崎大学, 大学院・医歯薬学総合研究科, 教授 (30039656)
• Co-Investigator(Kenkyū-buntansha): WADA Mitsuhiro 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (40295093)
• Project Period (FY): 2009 – 2011
• Keywords: 分析化学 / 臨床分析化学

Research Abstract

A ruthenium(II)-bipyridine complex chemiluminescent measurement of folic acid related compounds has been examined and developed a useful HPLC-CL method. The developed method was successfully applied to quantify folic acid in tablets and supplements. This means the method might be useful for the quality control of these materials. On the other hand, application of the method to folic acid related compounds except for 5-methyltetrahydrofolic acid in biomaterials was not always successful due to the less stability of these compounds and the lower sensitivity of the method. Improvement of the method concerning the conditions such as stabilizing agent and so on, are required.

Research Products

• [Journal Article] HPLC-ルテニウム錯体化学発光定量による葉酸含有医薬品及びサプリメントの分析 (2012)
  • Author(s): 田渕直人、池田理恵、和田光弘、黒田直敬、中島憲一郎
  • Journal Title: 長崎県総合公衆衛生研究会誌 Volume: 44 Pages: 12-13
• [Journal Article] Development and application of sensitive methods with luminescence detection of biologically active compounds (2011)
  • Author(s): K. Nakashima
  • Journal Title: J. Health Sci. Volume: 57 Pages: 10-21
  • Peer Reviewed
• [Journal Article] The association between atherosclerosis and plasma homocycteine concentration in the general population residing on remote islands in Japan (2011)
  • Author(s): M. Nakazato, N. Takamura, K. Kadota, H. Yamasaki, H. Mukae, Y. Kusano, K. Nakashima, Y. Ozono, K. Aoyagi, S. Kohno, T. Maeda
  • Journal Title: Acta Med. Nagasaki Volume: 55 Pages: 47-54
  • Peer Reviewed
• [Journal Article] 蛍光および化学発光法と臨床分析化学 (2010)
  • Author(s): 和田光弘、中島憲一郎
  • Journal Title: 臨床化学 Volume: 39(1) Pages: 6-14
  • Peer Reviewed
• [Journal Article] A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasma (2009)
  • Author(s): S. Ichinose, M. Nakamura, M. Maeda, R. Ikeda, M. Wada, M. Nakazato, Y. Ohba, N. Takamura, T. Maeda, R. Ikeda, K. Aoyagi, K. Nakashima
  • Journal Title: Biomed. Chromatogr. Volume: 23 Pages: 935-939
  • Peer Reviewed
• [Presentation] (2012)
  • Author(s): 田渕直人、池田理恵、和田光弘、黒田直敬、中島憲一郎
  • Organizer: 第49回長崎県公衆衛生研究会
  • Place of Presentation: 長崎
  • Year and Date: 20120000
• [Presentation] ルテニウム錯体化学発光を用いる新規葉酸定量法の開発の関する基礎的検討 (2010)
  • Author(s): 一山公佑、池田理恵、和田光弘、前田隆浩、中島憲一郎
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 20100000
• [Presentation] ルテニウム錯体化学発光によるサプリメント錠剤中葉酸の定量 (2010)
  • Author(s): 一山公佑、池田理恵、和田光弘、前田隆浩、中島憲一郎
  • Organizer: 第27回日本薬学会九州支部大会
  • Place of Presentation: 長崎
  • Year and Date: 20100000
• [Presentation] ルテニウム錯体-Ce化学発光系による葉酸の簡便分析法の開発 (2010)
  • Author(s): 中島憲一郎、一山公佑、池田理恵、和田光弘、前田隆浩、黒田直敬
  • Organizer: 生物発光化学発光研究会第27会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 20100000
• [Presentation] 健常人血漿チオール化合物の定量とその解析 (2009)
  • Author(s): 前田麻由、中村真裕美、池田理恵、和田光弘、中里未央、前田隆浩、高村昇、青柳潔、中島憲一郎
  • Organizer: 第49回日本臨床化学会年次学術集会
  • Place of Presentation: 長崎
  • Year and Date: 20090000