2011 Fiscal Year Final Research Report
Identification of endo-type chondroitin sulfate hydrolases applicable to neuronal regenerative therapy
| Project/Area Number |
21590057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMADA Shuhei 北海道大学, 大学院・先端生命科学研究院, 准教授 (70240017)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGAHARA Kazuyuki 北海道大学, 大学院・先端生命科学研究院, 教授 (60154449)
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| Project Period (FY) |
2009 – 2011
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| Keywords | グリコサミノグリカン / コンドロイチン硫酸 / ヒアルロン酸 / 神経再生 / ヒアル |
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| Research Abstract |
In this study, human hyaluronidase-4 (HYAL4) was demonstrated to be a chondroitin sulfate (CS)-specific endo-type hydrolase for the first time. Although mouse hyaluronidase-4 (mHyal4) was also demonstrated to be a CS-specific endo-type hydrolase, it has broader substrate specificity than HYAL4. A series of human/mouse hyaluronidase-4 chimeric proteins and point mutants were generated, and the amino acid residues responsible for the difference in substrate specificity were identified. To prepare an active enzyme applicable to neuronal regenerative therapy in a large scale, the expression system in yeast was constructed. Injury region was extracted from the rats with lateral hemisection of the spinal cord, and the composition of CS in the region as well as its sensitivity to mHyal4 was investigated.
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[Journal Article] Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus2011
Author(s)
Jiancheng Chen, Shuhei Yamada#, Yoshiki Hama, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Eunmi Kim, Takashi Kimura, Naonori Takahashi, Kazuya I. P. J. Hidari, Takashi Suzuki, Yasuo Suzuki, and Kazuyuki Sugahara#
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 412(1)
Pages: 136-142
Peer Reviewed
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[Journal Article] Important Role of Heparan Sulfate in Postnatal Islet Growth and Insulin Secretion2009
Author(s)
Iwao Takahashi, Naoya Noguchi, Koji Nata, Shuhei Yamada, Tomoyuki Kaneiwa, Shuji Mizumoto, Takayuki Ikeda, Kazushi Sugihara, Masahide Asano, Takeo Yoshikawa, Akiyo Yamauchi, Nausheen Jamal Shervani, Akira Uruno, Ichiro Kato, Michiaki Unno, Kazuyuki Sugahara, Shin Takasawa, Hiroshi Okamoto, and Akira Sugawara
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 383(1)
Pages: 113-118
Peer Reviewed
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