2011 Fiscal Year Final Research Report
Understanding of glycosylation functions in activated lymphocytes
| Project/Area Number |
21590063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKEMATSU Hiromu 京都大学, 大学院・生命科学研究科, 准教授 (80324680)
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| Project Period (FY) |
2009 – 2011
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| Keywords | シアル酸 / 糖鎖 / レクチン / 認識 / リンパ球 / 胚中心 |
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| Research Abstract |
In this grant, I explored functional aspects of N-glycolylneuraminic acid(Neu5Gc) in mouse lymphocytes to understand immunological function of sialic acid species modulation. Targeted disruption of CD22 and Neu5Gc-biosynthesis enzyme CMP-Neu5Ac hydroxylase(CMAH) resulted in the non-overlapping phenotypes in lymphocytes. Obtained data indicated that Neu5Gc, although that serves as high affinity glycan ligand for CD22, functions partly apart from CD22 ligand. This research results indicated that Neu5Gc has CD22-independent cellular autonomous function(s), that may not be limited in lymphocytes. Therefore, when mice were utilized as a model for human in biomedical research, it is strongly recommended to use cmah-null background to avoid confusion in the phenotypic analysis.
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[Journal Article] CD22-Antagonists with nanomolar potency : The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold2011
Author(s)
Abdu-Allah HHM, Watanabe K, Completo GC, Sadagopan M, Hayashizaki K, Takaku C, Tamanaka T, Takematsu H, Kozutsumi Y, Paulson JC, Tsubata T, Ando H, Ishida H, Kiso M
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Journal Title
Bioorg and Med Chem
Volume: 19(6)
Pages: 1966-71
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[Journal Article] B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase2009
Author(s)
Cariappa A, Takematsu H, Liu H, Diaz S, Haider K, Boboila C, Kalloo G, Connole M, Shi HN, Varki N, Varki A, Pillai S.
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Journal Title
J Exp Med
Volume: 206(1)
Pages: 125-38
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[Journal Article] Glycosphingolipids are not pivotal receptors for Subtilase cytotoxin in vivo : sensitivity analysis with glycosylation-defective mutant mice2009
Author(s)
Kondo Y, Tokuda N, Fan X, Yamashita T, Honke K, Takematsu H, Togayachi A, Ohta M, Kozutsumi Y, Narimatsu H, Tajima O, Furukawa K, Furukawa K.
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Journal Title
Biochem Biophys Res Commun
Volume: 378(2)
Pages: 179-81
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