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2011 Fiscal Year Final Research Report

Analyses of reverse membrane invaginations regulated by ubiquitination-dependent mechanism

Research Project

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Project/Area Number 21590064
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKyushu University

Principal Investigator

FUJITA Hideaki  九州大学, 大学院・薬学研究院, 助教 (80291524)

Co-Investigator(Renkei-kenkyūsha) TOKUNAGA Kenzo  国立感染症研究所, 感染病理部, 主任研究官 (50342895)
Project Period (FY) 2009 – 2011
Keywordsリソソーム / ユビキチン / タンパク質分解 / 小胞輸送 / エンドソーム / トランスフェリン受容体 / 鉄代謝 / 細胞質分裂
Research Abstract

We found a novel mechanism of down-regulation of transferring receptor. We characterized an intracellular logistics of BST-2/Tetherin, which is a host restriction factor of retrovirus. We found that Vpu, an accessory protein of HIV-1, counteracts a role of BST-2/Tetherin. We further analyzed the mechanisms of two novel whitening reagents in melanocytes.

  • Research Products

    (26 results)

All 2012 2011 2010 2009

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (17 results)

  • [Journal Article] Intracellular logistics of BST-2/Tetherin2012

    • Author(s)
      H. Fujita, K. Fujimoto, K. Tokunaga and Y. Tanaka.
    • Journal Title

      Curr HIV Res

    • Peer Reviewed
  • [Journal Article] Proteome of ubiquitin/MVB-Pathway : Possible involvement of Iron-induced ubiquitylation of transferrin receptor in lysosomal degradation2011

    • Author(s)
      R. Tachiyama, D. Ishikawa, M. Matsumoto, K. I Nakayama, T. Yoshimori, S. Yokota, M. Himeno, Y. Tanaka and H. Fujita*
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 448-466

    • Peer Reviewed
  • [Journal Article] Inhibitory effects of 16-hydroxy-9-oxo-10E, 12E, 14E-octade catrienoic acid(Corchorifatty acid B) isolated from Melissa officinalis Linne on melanogenesis.2011

    • Author(s)
      H. Fujita, M. Hongo, M. Mochizuki, K. Yokoyama and Y. Tanaka.
    • Journal Title

      Experimental Dermatology

      Volume: 20 Pages: 420-424

    • Peer Reviewed
  • [Journal Article] Differential Anti-APOBEC3G Activity of HIV-1 Vif Proteins Derived from Different Subtypes2010

    • Author(s)
      Y. Iwabu, M. Kinomoto, M. Tatsumi, H. Fujita, M. Shimura, Y. Tanaka, Y. Ishizaka, D. Nolan, S. Mallal, T. Sata, and K. Tokunaga.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 35350-35358

    • Peer Reviewed
  • [Journal Article] Nedd4-interactin protein 2(NDFIP2), a short half-life lysosomal membrane protein2010

    • Author(s)
      C. Ohzono, S. Etoh, M. Matsumoto, K. I. Nakayama, Y. Hirota, Y. Tanaka and H. Fujita§
    • Journal Title

      Biol. Pharm. Bull

      Volume: 33 Pages: 951-957

    • Peer Reviewed
  • [Journal Article] "Direct internalization of cell-surface BST-2/tetherin by the HIV-1 accessory protein Vpu2010

    • Author(s)
      Y. Iwabu, H. Fujita, Y. Tanaka, T. Sata, and K. Tokunaga.
    • Journal Title

      Communicative & Integrative Biology

      Pages: 366-369

    • Peer Reviewed
  • [Journal Article] HIV-1 accessory protein Vpu internalizes cell-surface BST-2/tetherin through transmembrane interactions leading to lysosomes2009

    • Author(s)
      Y. Iwabu, H. Fujita, M. Kinomoto, K. Kaneko, Y. Ishizaka, Y. Tanaka, T. Sata, and K. Tokunaga.
    • Journal Title

      J. Biol. Chem

      Volume: 284 Pages: 35060-35072

    • Peer Reviewed
  • [Journal Article] HM1. 24 is internalized from lipid rafts by clathrin-mediated endocytosis through interaction with alpha-adaptin2009

    • Author(s)
      N. Masuyama, T. Kuronita, R. Tanaka, T. Muto, Y. Hirota, A. Takigawa, H. Fujita, Y. Aso, J. Amano and Y. Tanaka.
    • Journal Title

      J. Biol. Chem

      Volume: 284 Pages: 15927-41

    • Peer Reviewed
  • [Journal Article] Inulavosin, a melanogenesis inhibitor, leads a mistargeting of tyrosinase to lysosome and accelerates its degradation2009

    • Author(s)
      H. Fujita, T. Motokawa, T. Katagiri, S. Yokota, A. Yamamoto, M. Himeno and Y. Tanaka.
    • Journal Title

      J. Investig. Dermatol

      Volume: 129 Pages: 1489-99

    • Peer Reviewed
  • [Presentation] 物誘発性リン脂質症の分子機構に関する研究2012

    • Author(s)
      廣田有子、藤本景子、栗原俊祐、阿佐絵里佳、池田一彦、佐野孝一、神吉将之、関二郎、藤田英明、田中嘉孝
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎大学
    • Year and Date
      20121200
  • [Presentation] Targeting of recycling endosomes to the cleavage furrow requires ubiquitylation of membrane proteins2012

    • Author(s)
      H. Fujita, Y. Iwabu, K. Tokunaga, T. Sata, K. Fujimoto, R. Tachiyama, D. Ishikawa, M. Matsumoto, K. I. Nakayama, and Y. Tanaka.
    • Organizer
      AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY Special Symposium"BIOCHEMISTRY AND CELL BIOLOGY OF ESCRTS IN HEALTH AND DISEASE
    • Place of Presentation
      Utah, USA
    • Year and Date
      20121000
  • [Presentation] Direct internalization of cell-surface BST-2/tetherin by HIV-1 Vpu2012

    • Author(s)
      Y. Iwabu, H. Fujita, Y. Tanaka, T. Sata, and K. Tokunaga.
    • Organizer
      CRM 2010-Centennial Retrovirus Meeting
    • Place of Presentation
      Institute of Molecular Genetics, Prague, Czech Republic
    • Year and Date
      20120400
  • [Presentation] 膜結合型ユビキチンリガーゼMARCH8によるトランスフェリン受容体ダウンレギュレーションの分子機構2012

    • Author(s)
      藤田英明、岩部幸枝、佐多徹太郎、徳永研三、田中嘉孝
    • Organizer
      日本薬学会第132年会・シンポジウム「メタロミクス研究の新展開-金属元素の1細胞分析から疾患との関連まで-
    • Place of Presentation
      札幌
    • Year and Date
      20120300
  • [Presentation] 膜結合型ユビキチンリガーゼMARCH8によるトランスフェリン受容体のユビキチン化およびダウンレギュレーションの分子機構2011

    • Author(s)
      藤田英明、岩部幸枝、佐多徹太郎、徳永研三、田中嘉孝
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110900
  • [Presentation] Trans-Golgi network膜タンパク質TGN46の細胞内輸送および局在化の分子制御機構2011

    • Author(s)
      藤本景子、吉永奈央、平井祥一、藤田英明、廣田有子、田中嘉孝
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110900
  • [Presentation] 「ユビキチン化による金属輸送体の細胞内輸送制御2011

    • Author(s)
      藤田英明、立山諒、石川大輔、松本雅記、中山敬一、横田貞記、吉森保、姫野勝、田中嘉孝
    • Organizer
      「ユビキチン化による金属輸送体」日本薬学会第131年会・特別シンポジウムE「トキシコメタロミクスの新機軸.元素分析・イメージングと金属輸送・創薬のイノベーション
    • Year and Date
      20110300
  • [Presentation] 鉄濃度依存的なトランスフェリン受容体のユビキチン化による代謝回転制御に関する研究2010

    • Author(s)
      藤田英明、立山諒、松本雅記、中山敬一、吉森保、姫野勝、横田貞記、田中嘉孝
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎大学
    • Year and Date
      20101200
  • [Presentation] 「Trans-Golgi network膜タンパク質TGN46の細胞内輸送および局在化の分子制御機構に関する研究2010

    • Author(s)
      藤本景子、吉永奈央、平井祥一、廣田有子、静川直子、藤田英明、田中嘉孝
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎大学
    • Year and Date
      20101200
  • [Presentation] The endosomal vesicles carrying ubiquitylated cargos selectively localizes to the cleavage furrow during cytokinesis2010

    • Author(s)
      H. Fujita, Y. Iwabu, K. Tokunaga, T. Sata, K. Fujimoto, R. Tachiyama, D. Ishikawa, M. Matsumoto, K. I. Nakayama, and Y. Tanaka.
    • Organizer
      第83回日本生化学会大会/第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] BST-2/tetherinのdownregulationにおけるHIV-1 Vpuのcofactor要求性2010

    • Author(s)
      岩部幸枝,藤田英明,田中嘉孝,佐多徹太郎,徳永研三
    • Organizer
      第83回日本生化学会大会/第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] HIV-1 Vpu internalizes cell-surface BST-2/tetherin and leads it to lysosomes2010

    • Author(s)
      Y. Iwabu, H. Fujita, M. Kinomoto, K. Kaneko, Y. Ishizaka, Y. Tanaka, T. Sata and K. Tokunaga
    • Organizer
      The 17^<th> Conference on Retroviruses and Opportunistic Infections
    • Place of Presentation
      Sam Francisco, CA, USA
    • Year and Date
      20100200
  • [Presentation] Inhibitory effect of active compound isolated from Melissa officinalis Linne(Labiatae) on melanogenesis and phagocytosis2009

    • Author(s)
      M. Hongo, H. Fujita, M. Mochizuki, T. Kato, K. Yokoyama, Y. Tanaka.
    • Organizer
      34^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
    • Place of Presentation
      Workshop, Fuluoka
    • Year and Date
      20091200
  • [Presentation] BST-2/TetherinとHIV-1 Vpuの相互作用における結合領域の同定2009

    • Author(s)
      岩部幸枝、藤田英明、石坂幸人、田中嘉孝、佐多徹太郎、徳永研三
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20091200
  • [Presentation] HIV-1 VpuとBST-2/TetherinのTransmembrane領域間における相互作用2009

    • Author(s)
      岩部幸枝、藤田英明、石坂幸人、田中嘉孝、佐多徹太郎、徳永研三
    • Organizer
      第57回日本ウイルス学会
    • Place of Presentation
      東京
    • Year and Date
      20091000
  • [Presentation] HIV-1 Vpuによるウイルス放出抑制因子BST-2/Tetherinの細胞内分解経路の解明2009

    • Author(s)
      岩部幸枝、藤田英明、石坂幸人、田中嘉孝、佐多徹太郎、徳永研三
    • Organizer
      第57回日本ウイルス学会
    • Place of Presentation
      東京
    • Year and Date
      20091000
  • [Presentation] 過剰鉄によるユビキチン化がトランスフェリン受容体のリソソームでのダウンレギュレーションを引き起こす2009

    • Author(s)
      藤田英明、立山諒、石川大輔、松本雅記、中山敬一、横田貞記、吉森保、姫野勝、田中嘉孝
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      20091000

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Published: 2013-07-31  

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