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2011 Fiscal Year Final Research Report

Analysis of regulatory mechanisms of Protein L-isoaspartyl(D-aspartyl) O-methyltransferase(PIMT) gene expression.

Research Project

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Project/Area Number 21590069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKitasato University

Principal Investigator

FURUCHI Takemitsu  北里大学, 薬学部, 准教授 (00302167)

Project Period (FY) 2009 – 2011
Keywords分子生物学
Research Abstract

In order to clarify the transcriptional regulatory mechanisms of PIMT, isomerized protein repair enzyme, we cloned and functionally characterized the 5'-flanking(promoter) region of the gene. The putative promoter activity was confirmed using dual luciferase reporter gene assay system. The minimal region required for basal activity of the promoter was determined by generating a series of deletion and point mutation constructs. The binding protein(s) to the minimal region was briefly purified using biotin-labeled DNA probe, and subsequently LC-MS/MS analysis was performed and a transcriptional factor, NRF1, was identified. Binding activity of NRF1 to the minimal region was confirmed using electrophoretic mobility shift assay and chromatin immunoprecipitation assay.

  • Research Products

    (17 results)

All 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during development in Caenorhabditis elegans2012

    • Author(s)
      Saitoh Y, Katane M, Kawata T, Maeda K, Sekine M, Furuchi T, Kobuna H, Sakamoto T, Inoue T, Arai H, Nakagawa Y, Homma H
    • Journal Title

      Mol Cell Biol

      Volume: 32(10) Pages: 1967-1983

    • DOI

      DOI:10.1128/MCB.06513-11

    • Peer Reviewed
  • [Journal Article] Role of the active site residues arginine-216 and arginine-237 in the substrate specificity of mammalian D-aspartate oxidase2011

    • Author(s)
      Katane M, Saitoh Y, Maeda K, Hanai T, Sekine M, Furuchi T, Homma H
    • Journal Title

      Amino Acids

      Volume: 40(2) Pages: 467-476

    • DOI

      DOI:10.1007/s00726-010-0658-4

    • Peer Reviewed
  • [Journal Article] Thiolactomycin inhibits D-aspartate oxidase : a novel approach to probing the active site environment2010

    • Author(s)
      Katane M, Saitoh Y, Hanai T, Sekine M, Furuchi T, Koyama N, Nakagome I, Tomoda H, Hirono S, Homma H
    • Journal Title

      Biochimie

      Volume: 92(10) Pages: 1371-1378

    • DOI

      DOI:10.1016/j.biochi.2010.06.021

    • Peer Reviewed
  • [Journal Article] Comparative characterization of three D-aspartate oxidases and one D-amino acid oxidase from Caenorhabditis elegans2010

    • Author(s)
      Katane M, Saitoh Y, Seida Y, Sekine M, Furuchi T, Homma H
    • Journal Title

      Chem Biodivers

      Volume: 7(6) Pages: 1424-1434

    • DOI

      DOI:10.1002/cbdv.200900294

    • Peer Reviewed
  • [Journal Article] The role of protein L-isoaspartyl/D-aspartyl O-methyltransferase(PIMT) in intracellular signal transduction2010

    • Author(s)
      Furuchi T, Sakurako K, Katane M, Sekine M, Homma H
    • Journal Title

      Chem Biodivers

      Volume: 7(6) Pages: 1337-1348

    • DOI

      DOI:10.1002/cbdv.200900273

    • Peer Reviewed
  • [Journal Article] Apoptotic inducers activate the release of D-aspartate through a hypotonic stimulus-triggered mechanism in PC12 cells2009

    • Author(s)
      Furuchi T, Suzuki T, Sekine M, Katane M, Homma H
    • Journal Title

      Arch Biochem Biophys

      Volume: 490(2) Pages: 118-128

    • DOI

      DOI:10.1016/j.abb.2009.08.017

    • Peer Reviewed
  • [Presentation] Protein L-isoaspartyl/D-aspartyl-o-methyltransferase(PIMT)の転写調節機構の解析2011

    • Author(s)
      古地壯光、本間浩
    • Organizer
      第84回日本生化学会大会(京都)シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
  • [Presentation] 異性化タンパク質修復酵素PIMTの転写因子の同定2011

    • Author(s)
      立石秀樹、古地壯光、原田怜、清水由香里、伊藤耕平、片根真澄、関根正恵、太田安隆、本間浩
    • Organizer
      第7回D-アミノ酸研究会学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-09
  • [Presentation] Identification of the transcription factor(s) responsible for Protein L-isoaspartyl(D-aspartyl) O-methyltransferase(PIMT) gene expression2011

    • Author(s)
      Takemitsu Furuchi, Satoru Harada, Kohei Ito, Shimizu Yukari, Masumi Katane, Masae Sekine, Yasutaka Ohta, Hiroshi Homma
    • Organizer
      23rd Biennial Joint Meeting of the International Society for Neurochemistry(ISN) and the European Society for Neurochemistry(ESN)
    • Place of Presentation
      Athens, Greece
    • Year and Date
      2011-08-29
  • [Presentation] Protein L-isoaspartyl(D-aspartyl) O-methyltransferase(PIMT)の転写調節に関わる因子の探索2010

    • Author(s)
      伊藤耕平、古地壯光、原田怜、清水由香里、片根真澄、関根正恵、太田安隆、本間浩
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] 異性化タンパク質修復酵素PIMTの転写調節因子の検索2010

    • Author(s)
      原田怜、古地壯光、清水由香里、伊藤耕平、片根真澄、関根正恵、太田安隆、本間浩
    • Organizer
      第6回D-アミノ酸研究会学術講演会
    • Place of Presentation
      富山
    • Year and Date
      2010-09-18
  • [Presentation] Protein L-isoaspartyl/D-aspartyl-o-methyltransferase(PIMT)の転写活性化に必須な領域の同定2010

    • Author(s)
      原田怜、古地壯光、清水由香里、片根真澄、関根正恵、本間浩
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-28
  • [Presentation] Simple high-performance liquid chromatography-fluorescence detection method to measure protein L-isoaspartyl/D-aspartyl O-methyltransferase activity in cell lysates2009

    • Author(s)
      Takemitsu Furuchi, Sakurako Kosugi, Tsukasa Egawa, Keiko Ohno, Masae, Sekine, Masumi Katane, Hiroshi Homma
    • Organizer
      21th IUBMB and 12th FAOBMB International congress of biochemistry and molecular biology
    • Place of Presentation
      Shanghai, China
    • Year and Date
      20090803-04
  • [Presentation] Protein L-isoaspartyl/D-aspartyl methyltransferase(PIMT)プロモーターの転写制御に関わる転写因子の探索2009

    • Author(s)
      清水由香里、古地壯光、原田怜、小杉桜子、片根真澄、関根正恵、本間浩
    • Organizer
      第53回日本薬学会関東支部大会
    • Place of Presentation
      坂戸
    • Year and Date
      2009-10-03
  • [Presentation] Characterization of the promoter region of the protein L-isoaspartyl/D-aspartyl O-methyltransferase(PIMT) gene2009

    • Author(s)
      Yukari Shimizu, Takemitsu Furuchi, Sakurako Kosugi, Masumi Katane, Masae Sekine, Hiroshi Homma
    • Organizer
      The first international conference of D-amino acid research
    • Place of Presentation
      Awaji, Hyogo
    • Year and Date
      2009-07-03
  • [Presentation] Analysis of mechanisms of EDF-triggered hyperactivation of ERK in L-isoaspartyl/D-aspartyl O-methyltransferase(PIMT)-knockdown cells.2009

    • Author(s)
      Takemitsu Furuchi, Sakurako Kosugi, Satoru Harada, Masumi Katane, Masae Sekine, Hiroshi Homma
    • Organizer
      The first international conference of D-amino acid research
    • Place of Presentation
      Awaji, Hyogo
    • Year and Date
      2009-07-02
  • [Remarks]

    • URL

      http://www.pharm.kitasato-u.ac.jp/ac/SeitaiHP/

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Published: 2013-07-31  

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