2011 Fiscal Year Final Research Report
Study of spatiotemporal expression of D-amino acid oxidases and D-amino acid contents in Caenorhabditis elegans
| Project/Area Number |
21590071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生化学 |
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| Research Abstract |
Caenorhabditis elegans, a model organism has four genes which encode the enzymes degrading D-amino acids oxidatively. One of them encodes D-amino acid oxidase which degrades neutral and basic amino acids, while the other three are D-aspartate oxidases 1-3 degrading acidic D-amino acids. In this work, I isolated and prepared the mutants defective in each gene, and compared them with the wild type strain. First I determined by HPLC the contents of D-amino acids in the body of wild type and the mutants. Then I examined the phenotypes and behaviors of the mutants and compared with those of the wild type. Based on these results I discussed the physiological roles of D-amino acids.
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