2011 Fiscal Year Final Research Report
Identification of a gene that can control expression of endoderm-specific genes
| Project/Area Number |
21590089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Biomedical Innovation |
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Principal Investigator |
KAWABATA Kenji 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (50356234)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Fuminori 大阪大学, 大学院・薬学研究科, 准教授 (70370939)
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| Project Period (FY) |
2009 – 2011
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| Research Abstract |
We explored a novel gene that is involved in endodermal differentiation by using a human iPS cell-differentiation system. We found that expression of Runx1 gene was disappeared when cells were differentiated from the mesendoderm stage to definitive endoderm stage. Also, Runx1 could negatively regulate the expression of endoderm-specific genes, such as Foxa2 and Sox17, by directly binding the regulatory regions of their genes. In contrast, when the function of Runx1 was inhibited by its dominant-negative mutant, expression levels of Foxa2, Sox17, GATA4 genes were increased. Therefore, Runx1 is important for not only mesoderm differentiation but also endoderm differentiation.
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Research Products
(18 results)
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[Journal Article] Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4αtransduction2012
Author(s)
Takayama K., Inamura M., Kawabata K., Katayama K., Higuchi M., Tashiro K., Nonaka A., Sakurai F., Hayakawa T., Furue MK., Mizuguchi H.
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Journal Title
Mol. Ther.
Volume: 20
Pages: 127-137
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[Journal Article] Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX2011
Author(s)
Inamura M., Kawabata K., Takayama K., Tashiro K., Sakurai F., Katayama K., Toyoda M., Akutsu H., Miyagawa Y., Okita H., Kiyokawa N., Umezawa A., Hayakawa T., Furue MK., Mizuguchi H.
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Journal Title
Mol. Ther.
Volume: 19
Pages: 400-407
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