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2011 Fiscal Year Final Research Report

Identification of a gene that can control expression of endoderm-specific genes

Research Project

  • PDF
Project/Area Number 21590089
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionNational Institute of Biomedical Innovation

Principal Investigator

KAWABATA Kenji  独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (50356234)

Co-Investigator(Kenkyū-buntansha) SAKURAI Fuminori  大阪大学, 大学院・薬学研究科, 准教授 (70370939)
Project Period (FY) 2009 – 2011
Research Abstract

We explored a novel gene that is involved in endodermal differentiation by using a human iPS cell-differentiation system. We found that expression of Runx1 gene was disappeared when cells were differentiated from the mesendoderm stage to definitive endoderm stage. Also, Runx1 could negatively regulate the expression of endoderm-specific genes, such as Foxa2 and Sox17, by directly binding the regulatory regions of their genes. In contrast, when the function of Runx1 was inhibited by its dominant-negative mutant, expression levels of Foxa2, Sox17, GATA4 genes were increased. Therefore, Runx1 is important for not only mesoderm differentiation but also endoderm differentiation.

  • Research Products

    (18 results)

All 2012 2011 2010 Other

All Journal Article (3 results) Presentation (11 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4αtransduction2012

    • Author(s)
      Takayama K., Inamura M., Kawabata K., Katayama K., Higuchi M., Tashiro K., Nonaka A., Sakurai F., Hayakawa T., Furue MK., Mizuguchi H.
    • Journal Title

      Mol. Ther.

      Volume: 20 Pages: 127-137

  • [Journal Article] Efficient and selective generation of two distinct endoderm lineages from human ES and iPS cells by differentiation stage-specific SOX17 transduction2011

    • Author(s)
      Takayama K., Inamura M., Kawabata K., Tashiro K., Katayama K., Hayakawa T., Furue MK., Mizuguchi H.
    • Journal Title

      PLoS One

      Volume: 6 Pages: e21780

  • [Journal Article] Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX2011

    • Author(s)
      Inamura M., Kawabata K., Takayama K., Tashiro K., Sakurai F., Katayama K., Toyoda M., Akutsu H., Miyagawa Y., Okita H., Kiyokawa N., Umezawa A., Hayakawa T., Furue MK., Mizuguchi H.
    • Journal Title

      Mol. Ther.

      Volume: 19 Pages: 400-407

  • [Presentation] FOXA2・遺伝子導入によるヒト多能性幹細胞から薬剤代謝能を有した肝細胞の分化誘導2012

    • Author(s)
      高山和雄、稲村充、川端健二、菅原道子、菊池きよ美、櫻井文教、古江-楠田美保、水口裕之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      20120328-31
  • [Presentation] 3次元共培養法によるヒトES・iPS細胞由来肝細胞の効率的な分化誘導法の開発2012

    • Author(s)
      長基康人、田代克久、高山和雄、大橋一夫、櫻井文教、立花雅史、古江(楠田)美保、川端健二、水口裕之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      20120328-31
  • [Presentation] EFFICIENT GENERATION OF MATURE HEPATOCYTES FROM HUMAN PLURIPOTENT STEM CELLS BY HNF4αTRANSDUCTION2011

    • Author(s)
      Kazuo Takayama, Mitsuru Inamura, Kenji Kawabata, Kazufumi Katayama, Katsuhisa Tashiro, Fuminori Sakurai, Miho Kusuda Furue, Hiroyuki Mizuguchi
    • Organizer
      第26回日本薬物動態学会年
    • Place of Presentation
      広島
    • Year and Date
      20111116-18
  • [Presentation] HNF4遺伝子導入によるヒトES・iPS細胞からの成熟肝細胞への高効率分化誘導2011

    • Author(s)
      高山和雄、稲村充、川端健二、田代克久、形山和史、櫻井文教、古江-楠田美保、水口裕之
    • Organizer
      第18回肝細胞研究会
    • Place of Presentation
      東京
    • Year and Date
      20110624-25
  • [Presentation] HNF4αpromotes hepatic maturation from human embryonic stem cell-derived hepatoblasts.2011

    • Author(s)
      Kazuo Takayama, Mitsuru Inamura, Kenji Kawabata, Kazufumi Katayama, Katsuhisa Tashiro, Fuminori Sakurai, Miho Kusuda Furue, Hiroyuki Mizuguchi
    • Organizer
      The 6th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      20110600
  • [Presentation] EFFICIENT GENERATION OF FUNCTIONAL HEPATOCYTES FROM HUMAN EMBRYONIC STEM CELLS AND INDUCED PLURIPOTENT STEM CELLS BY HNF4αTRANSDUCTION2011

    • Author(s)
      Kazuo Takayama, Mitsuru Inamura, Kenji Kawabata, Kazufumi Katayama, Katsuhisa Tashiro, Fuminori Sakurai, Miho Kusuda Furue, Hiroyuki Mizuguchi
    • Organizer
      International Society for Stem Cell Research
    • Place of Presentation
      Tronto
    • Year and Date
      20110600
  • [Presentation] HNF4α遺伝子導入によるヒトES・iPS細胞からの成熟肝細胞への高効率分化誘導2011

    • Author(s)
      高山和雄、稲村充、川端健二、田代克久、形山和史、櫻井文教、古江-楠田美保、水口裕之
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      20110328-31
  • [Presentation] 遺伝子導入を用いたiPS細胞の肝細胞への高効率分化誘導法2011

    • Author(s)
      川端健二、水口裕之
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      東京
    • Year and Date
      20110301-02
  • [Presentation] SOX17遺伝子導入によるヒトES・iPS細胞からの内胚葉および胚体外内胚葉への選択的分化誘導2011

    • Author(s)
      高山和雄、稲村充、川端健二、田代克久、形山和史、櫻井文教、古江-楠田美保、水口裕之
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      東京
    • Year and Date
      20110301-02
  • [Presentation] HEX遺伝子の導入によるヒトES細胞やヒトiPS細胞からの効率良い肝幹細胞への分化誘導2010

    • Author(s)
      稲村充、川端健二、高山和雄、田代克久、形山和史、櫻井文教、古江-楠田美保、水口裕之
    • Organizer
      第16回肝細胞研究会
    • Place of Presentation
      秋田
    • Year and Date
      20100618-19
  • [Presentation] SOX17遺伝子導入によるヒトES・iPS細胞からの内胚葉および胚体外内胚葉への選択的分化誘導2010

    • Author(s)
      高山和雄、稲村充、田代克久、形山和史、櫻井文教、古江-楠田美保、川端健二、水口裕之
    • Organizer
      第16回肝細胞研究会
    • Place of Presentation
      秋田
    • Year and Date
      20100618-19
  • [Book] Efficient hepatic differentiation from human iPS cells by gene transfer. Methods Mol Biol2012

    • Author(s)
      Kawabata K., Inamura M., Mizuguchi H.
    • Total Pages
      826
  • [Book] iPS細胞への遺伝子導入を用いた分化誘導の最適化,薬学雑誌2010

    • Author(s)
      川端健二、田代克久、水口裕之
    • Total Pages
      130
  • [Book] 多能性幹細胞から肝細胞への分化誘導,再生医療業書

    • Author(s)
      高山和雄、川端健二、水口裕之
  • [Remarks]

    • URL

      http://www.nibio.go.jp/Proj3HP/index.html

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Published: 2013-07-31  

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