2011 Fiscal Year Final Research Report
Development of an accurate analysis method of the intestinal bacterial flora, and detect the causative bacteria of ulcerative colitis, search its effective antibiotics.
| Project/Area Number |
21590138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 微生物 / 感染症学 |
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| Research Abstract |
Total DNA extracted from the fecal sample of UC model mouse and the healthy mouse were analyzed by DGGE method. As a result, we could find some new bands which were specific for UC model mouse. Furthermore we could obtain 15,000～40,000 sequence read from each specimen by pyrosequencer(Roche 454-FLX-Titanium), and find the certain bacteria whose population was increasing in UC model mouse.
After orally given Clostridium sp. ID4 strain-one representative organism which was identified from UC model mouse-, we could find the inflammatory image in the lower portion of the large intestine.
From these data, we concluded that the certain bacteria founding this study were not a little related with the UC onset.
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