Establishment of human cell lines for blood-tissue barrier and application to elucidate mechanism of low-dose anti-cancer drugs

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590175
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Keio University
• Principal Investigator: NAKASHIMA Emi 慶應義塾大学, 薬学部, 教授 (90115254)
• Co-Investigator(Kenkyū-buntansha): TOMI Masatoshi 慶應義塾大学, 薬学部, 准教授 (30334717) ; NISHIMURA Tomohiro 慶應義塾大学, 薬学部, 助教 (40453518) ; KOSE Noriko 慶應義塾大学, 薬学部, 研究員 (60348612)
• Co-Investigator(Renkei-kenkyūsha): SAI Yoshimichi 金沢大学, 大学病院, 准教授 (40262589)
• Project Period (FY): 2009 – 2011
• Keywords: 薬物動態 / 代謝学

Research Abstract

In formation process of a blood-tissue barrier, there is an interaction between specific tissue cells and bone marrow(BM)-derived endothelial progenitor cells(EPC). An assay system using the established cell lines from the specific tissue is desirable to obtain reproducible analysis. By using an established bone marrow-derived vascular endothelium precursor cell line, TR-BME, docetaxel and paclitaxel directly inhibited EPC-initiated vasculogenesis at low(non-cytotoxic) concentrations, causing suppression of tumor growth.

Research Products

• [Journal Article] Mother-to-fetus transfer of antiviral drugs and the involvement of transporters at the placental barrier (2011)
  • Author(s): Tomi M, Nishimura T, Nakashima E.
  • Journal Title: J Pharm Sci Volume: 100 Pages: 3708-3718
  • DOI: DOI:10.1002/jps.22642
  • Peer Reviewed
• [Journal Article] 6-Mercaptopurine transport by equilibrative nucleoside transporters in conditionally immortalized rat syncytiotrophoblast cell lines TR-TBTs (2011)
  • Author(s): Lee NY, Sai Y, Nakashima E, Ohtsuki S, Kang YS.
  • Journal Title: J Pharm Sci Volume: 100 Pages: 3773-3782
  • DOI: DOI:10.1002/jps.22631
  • Peer Reviewed
• [Journal Article] Enhancement of zidovudine transfer to molt-4 cells, a human t-cell model, by dehydroepiandrosterone sulfate (2011)
  • Author(s): Nishimura T, Tanaka J, Tomi M, Seki Y, Kose N, Sai Y, Nakashima E.
  • Journal Title: J Pharm Sci Volume: 100 Pages: 3959-3967
  • DOI: DOI:10.1002/jps.22624
  • Peer Reviewed
• [Journal Article] Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats (2010)
  • Author(s): Higuchi K, Iizasa H, Sai Y, Horieya S, Lee K-E, Wada M, Deguchi M, Nishimura T, Wakayama T, Tamura A, Tsukita S, Kose N, Kang Y-S, Nakashima E.
  • Journal Title: Biol Pharm Bull Volume: 33 Pages: 1400-1406
  • DOI: DOI:10.1248/bpb.33.1400
  • Peer Reviewed
• [Journal Article] Proton-coupled erythromycin antiport at rat blood-placenta barrier (2010)
  • Author(s): Sai Y, Nishimura T, Ochi K, Tanaka N, Takagi A, Tomi M, Kose N, Kobayashi Y, Miyakoshi N, Kitagaki S, Mukai C, Nakashima E.
  • Journal Title: Drug Metab Dispos. Volume: 38 Pages: 1576-1581
  • DOI: DOI:10.1124/dmd.110.033266
  • Peer Reviewed
• [Journal Article] Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress (2010)
  • Author(s): Nishimura T, Sai Y, Fujii J, Muta M, Iizasa H, Tomi M, Deureh M, Kose N, Nakashima E.
  • Journal Title: Placenta Volume: 31 Pages: 1003-1009
  • URL: http://dx.doi.org/10.1016/j.placenta.2010.08.003
  • Peer Reviewed
• [Journal Article] Effect of low-dose paclitaxel and docetaxel on endothelial progenitor cells (2009)
  • Author(s): Muta M, Yanagawa T, Sai Y, Saji S, Suzuki E, Aruga T, Kuroi K, Matsumoto G, Toi M, Nakashima E.
  • Journal Title: Oncology Volume: 77 Pages: 182-191
  • DOI: DOI:10.1159/000236016
  • Peer Reviewed
• [Presentation] In vivo techniques for the evaluation of passages from Matamal and Fetal sides across the placental bar (2011)
  • Author(s): Asada T, Takanohashi T, Nishimura T, Tomi M, Nakashima E.
  • Organizer: Asian Federation for Pharmaceutical Sciences 2011
  • Place of Presentation: Kuala Lumpur Malaysia
  • Year and Date: 20111208-12
• [Presentation] CD4陽性Tリンパ球モデルMolt-4細胞への抗ウイルス薬ジドブジン取り込み機構と標的化 (2011)
  • Author(s): 西村友宏、田中純、登美斉俊、巨勢典子、中島恵美
  • Organizer: 日本薬剤学会第26年会
  • Place of Presentation: 東京
  • Year and Date: 20110529-31
• [Presentation] ラット胎盤関門細胞株におけるMitoxantroneによるEstrogen Receptorを介したABCG2発現誘導 (2011)
  • Author(s): 小田憲司、西村友宏、巨勢典子、登美斉俊、中島恵美
  • Organizer: 日本薬剤学会第26年会
  • Place of Presentation: 東京
  • Year and Date: 20110529-31
• [Presentation] 胎盤hypotaurine取り込みにおけるGABAトランスポーター(Slc6a13)の関与 (2010)
  • Author(s): 樋口慧、西村友宏、登美斉俊、巨勢典子、崔吉道、中島恵美
  • Organizer: 第5回トランスポーター研究会年会
  • Place of Presentation: 東京
  • Year and Date: 20100710-11
• [Presentation] ラット胎盤関門におけるエリスロマイシンのプロトン共役排出輸送 (2010)
  • Author(s): 崔吉道、西村友宏、越智香織、田中紀章、高木彰紀、登美斉俊、巨勢典子、中島恵美
  • Organizer: 第5回トランスポーター研究会年会
  • Place of Presentation: 東京
  • Year and Date: 20100710-11
• [Presentation] Ezrin欠損マウス胎盤・胎児で減少するhypotaurineの胎盤におけるトランスポーター同定 (2010)
  • Author(s): 樋口慧、西村友宏、登美斉俊、若山友彦、田村淳、月田早智子、曽我朋義、崔吉道、中島恵美
  • Organizer: 日本薬剤学会第25年会
  • Place of Presentation: 徳島
  • Year and Date: 20100512-14