Structural and morphology changes in the disease model after amnion stem cell transplantation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590212
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Toyama
• Principal Investigator: YOSHIDA Toshiko 富山大学, 大学院・医学薬学研究部(医学), 准教授 (00171421)
• Co-Investigator(Kenkyū-buntansha): NIKAIDO Toshio 富山大学, 大学院・医学薬学研究部(医学), 教授 (50180568) ; OKABE Motonori 富山大学, 大学院・医学薬学研究部(医学), 助教 (60283066) ; KOIKE Chika 富山大学, 大学院・医学薬学研究部(医学), 助教 (10523889)
• Project Period (FY): 2009 – 2011
• Keywords: ヒト羊膜細胞 / 疾患モデル / 分化誘導 / 糖尿病モデル / 脊髄損傷モデル / 骨欠損モデル / 骨分化

Research Abstract

The stem cells contained in an amnion epithelium cells and mesenchymal cells in the amnion membrane were purified. The stem cells were transported in to disease model.
In the diabetes model, blood glucose level became low temporarily and survival benefit was seen. In the cord injury model, in spite of having transplanted the mesenchymal cells one week after damage, the motor function was recovered significantly. In the bone deficit model, in spite of xenograft, rejection hardly started but the bone tissue has been reproducing.
The amnion stem cells were effective as a cell material of regeneration medicine.

Research Products

• [Journal Article] Use of a hyper-dried cross-linked amniotic membrane as initial therapy for corneal perforations (2011)
  • Author(s): Kitagawa K., Okabe M., Yanagisawa S., Zhang Xue-Yun., Nikaido T., and Hayashi A.
  • Journal Title: Jpn. J. Ophthalmol Volume: 55 Pages: 16-21
  • Peer Reviewed
• [Journal Article] Effect of hyperdry amniotic membrane patches attached over the bony surface of mastoid cavities in canal wall down tympanoplasty (2011)
  • Author(s): Shojaku H., Takakura H., Okabe M., Fujisaka M., Watanabe Y., and Nikaido T.
  • Journal Title: Laryngoscope Volume: 121(9) Pages: 1953-1957
  • Peer Reviewed
• [Journal Article] Cultured epithelial grafting using human amniotic membrane : the potential for using human amniotic epithelial cells as a cultured oral epithelium sheet (2011)
  • Author(s): Koike T., Yasuo M., Shimane T., Kobayashi H., Nikaido T., and Kurita H.
  • Journal Title: Arch. Oral Biol. Volume: 56(10) Pages: 1170-1176
  • Peer Reviewed
• [Journal Article] Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats. (2011)
  • Author(s): Kimura M., Shibahara N., Hikiami H., Yoshida T., Jo M., Kaneko M., Mogami T., Fujimoto M., Goto H., and Shimada Y.
  • Journal Title: Evid Based Complement Altemat Med Volume: 2011 Pages: 592791, 9
  • Peer Reviewed
• [Journal Article] Mechanisms of collagen fibril aligment in tendon injury : From tendon regeneration to artificial tendon (2011)
  • Author(s): Torigoe K., Tanaka HF., Yonenaga K., Ohkochi H., Miyasaka M., Sato R., Kusumaki T., Yoshida K., and Yoshida T.
  • Journal Title: J. Orhop. Res. Volume: 29 Pages: 1944-1950
  • Peer Reviewed
• [Journal Article] Hyaluronan tetrasaccharide promotes regeneration of peripheral nerve : in vivo analysis by film method (2011)
  • Author(s): Torigoe k., Tanak HF., Ohkochi H., Miyasaka M., Yamanokuchi H., Yoshida K., and Yoshida T.
  • Journal Title: Brain Res Volume: 18, 1385 Pages: 87-92
  • Peer Reviewed
• [Journal Article] Clinical Application of a Hyperdry Amniotic Membrane on Surgical Defects of the Oral Mucosa (2011)
  • Author(s): Arai N., Tsuno H., Okabe M., Yoshida T., Koike C., Noguchi M., and Nikaido T.
  • Journal Title: J Oral Maxillofac Surg
  • Peer Reviewed
• [Journal Article] Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells (2011)
  • Author(s): Teng Z., Yoshida T., Okabe M., Toda A., Higuchi 0., Nogami M., Yoneda N., Zhou K., Kyo S., Kiyono T., and Nikaido T.
  • Journal Title: Cell Transplant Volume: (in pres)
  • Peer Reviewed
• [Journal Article] New dried human amniotic membrane is useful as a substitute for dural repair after skull base surgery (2011)
  • Author(s): TomitaT., HayashiN., Okabe M., Yoshida T., Hamada H., Endo s., and Nikaido T.
  • Journal Title: Skull Base Volume: (in press)
  • Peer Reviewed
• [Journal Article] A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour (2010)
  • Author(s): Ito M., _Nakasima A., Hidaka T., Okabe M., Bac ND., Ina S., Yoneda S., Shiozaki A., Sumi S., Tuneyama K., Nikaido T., Saito S.
  • Journal Title: J Reprod Immunol Volume: 84 Pages: 75-85
  • Peer Reviewed
• [Journal Article] Human Amniotic Mesenchymal Cells Differentiate into Chondrocytes (2009)
  • Author(s): Wei J-P., Nawata M., Wakitani S., Kametani K., Ota M., Toda A., Konishi I., Ebara S., and Nikaido T.
  • Journal Title: Cloning and Stem Cells Volume: 11 Pages: 19-25
  • Peer Reviewed
• [Journal Article] A Hyperdry Amniotic Membrane Patch Using a Tissue Adhesive for Corneal Perforations and Bleb Leaks (2009)
  • Author(s): Kitagawa K., Yanagisawa S., Watanabe K., Yunoki T., Hayashi A., Okabe M., Nikaido T.
  • Journal Title: Am J Ophthalmol. Volume: 148 Pages: 383-389
  • Peer Reviewed
• [Journal Article] Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes. Molecular Human Renroduction (2009)
  • Author(s): Yoneda-Izumi N., Toda A., Okabe M., Koike C., Takashima S., Yoshida T., Konishi I., Saito S., Nikaido T.
  • Journal Title: Molecular Human Reproduction Volume: 15 Pages: 49-57
  • Peer Reviewed
• [Journal Article] Combined use of a novel dried cross-linked amniotic membrane and tissue adhesive to conjunctival defect following multiple trabeculectomy (2009)
  • Author(s): Kitagawa K., Okabe M., Hayashi A., Nikaido T.
  • Journal Title: Toyama Med J Volume: 20 Pages: 1-3
  • Peer Reviewed
• [Presentation] Reprogramming of human amniotic epithelial cells by Oct 4 overexpression (2012)
  • Author(s): Nikaido T., Takeda Y., Koike C., Yoshida T., and Okabe M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20121213-16
• [Presentation] バイオプリンティングロボットの次世代化:血管自動認識ロボット (2011)
  • Author(s): 岡部素典,荒井健一,吉田淑子,小池千加,杉本潤,戸田英樹,後藤光昭,中村真人,二階堂敏雄
  • Organizer: 第49回日本人工臓器学会大会
  • Place of Presentation: 東京
  • Year and Date: 20111125-27
• [Presentation] Application of Amnion Derived Cells to Tissue Engineering (2011)
  • Author(s): Koike C., Okabe M., Yoshida T., Toda H., Capi G, Nakamura M., and Nikaido T.
  • Organizer: International Conference on Biofabrication 2011 in TOYAMA
  • Place of Presentation: Toyama
  • Year and Date: 20111006-08
• [Presentation] 子宮頸癌における癌幹細胞の細胞表面マーカー (2011)
  • Author(s): 吉田淑子,齋藤滋,二階堂敏雄
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Presentation] 不死化羊膜問葉系細胞およびヒアルロン酸オリゴ糖(HA)投与による脊髄損傷治癒効果 (2011)
  • Author(s): 吉田淑子,岡部素典,小池千加,周凱旋,京哲,清野透,二階堂敏雄
  • Organizer: 第52回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 金沢
  • Year and Date: 20110924-25
• [Presentation] ヒト羊膜由来細胞の再生医療への応用 (2011)
  • Author(s): 二階堂敏雄,吉田淑子,岡部素典,小池千加
  • Organizer: 第29回日本ヒト細胞学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 20110820-21
• [Presentation] 脊髄損傷に対する不死化羊膜間葉系細胞の細胞治療材料としての有効性 (2011)
  • Author(s): 吉田淑子,岡部素典,吉田一晴,小池千加,周凱旋,京哲,清野透,齋藤滋,二階堂敏雄
  • Organizer: 第29回日本ヒト細胞学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 20110820-21
• [Presentation] 不死化羊膜細胞の樹立と解析 (2011)
  • Author(s): 岡部素典,吉田淑子,小池千加,齋藤滋,二階堂敏雄
  • Organizer: 第29回日本ヒト細胞学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 20110820-21
• [Presentation] 羊膜由来細胞のStemmnessの度合いの解析 (2011)
  • Author(s): 小池千加,周凱旋,岡部素典,吉田淑子,二階堂敏雄
  • Organizer: 第29回日本ヒト細胞学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 20110820-21
• [Presentation] 羊膜問葉系幹細胞の単離・同定と軟骨分化に有効な成長因子の検討 (2011)
  • Author(s): 野上真紀子*,津野宏彰, MoustafaFathyOmar,小池千加,岡部素典,吉田淑子,関庄二,松井好人,木村友厚,二階堂敏雄
  • Organizer: 第29回日本ヒト細胞学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 20110820-21
• [Presentation] 不死化羊膜上皮細胞の樹立と解析 (2011)
  • Author(s): 小池千加,周凱旋,吉田淑子,岡部素典,杉本潤,齋藤滋,清野透,京哲,二階堂敏雄
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 20110602-03
• [Presentation] 子宮頸癌における癌幹細胞の細胞表面マーカの同定(意義) (2011)
  • Author(s): 吉田淑子,王芳,岡部素典,小池千加,周凱旋,齋藤滋,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] 新規ヒト乾燥羊膜(Hyper-Dry羊膜)の臨床における有用性 (2011)
  • Author(s): 岡部素典,吉田淑子,小池千加,林央周,藤坂実千郎,将積日出夫,新井直也,津野宏彰,北川清隆,遠藤俊郎,渡邉行雄,野口誠,林篤志,齋藤滋,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] インスリン発現羊膜細胞の樹立とそれを用いた糖尿病治療の試み (2011)
  • Author(s): 小池千加,方晶,吉田淑子,周凱旋,岡部素典,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] 不死化羊膜上皮細胞の樹立と解析 (2011)
  • Author(s): 周凱旋*,小池千加,吉田淑子,岡部素典,京哲,清野透,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] Oct-4過剰発現による不死化ヒト羊膜上皮細胞のリプログラミング (2011)
  • Author(s): 竹田裕治*,小池千加,吉田淑子,岡部素典,杉本潤,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] ヒト羊膜幹細胞の存在部位の検討 (2011)
  • Author(s): 杉本潤,吉田淑子,岡部素典,小池千加,周凱旋,齋藤滋,林篤志,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] ヒト羊膜由来細胞の再生医療への応用 (2010)
  • Author(s): 二階堂敏雄
  • Organizer: 日本顕微鏡学会第54回シンポジウム
  • Place of Presentation: 金沢
  • Year and Date: 20101111-13
• [Presentation] Characterization of amniotic stem cells (2010)
  • Author(s): Koike C., Zhou K., Fathy M., Okabe M., Yoshida T., and Nikaido T.
  • Organizer: The 8th International Society for Stem Cell Research
  • Place of Presentation: San Francisco
  • Year and Date: 20100616-19
• [Presentation] 羊膜由来細胞を用いた組織構築の展望 (2010)
  • Author(s): 吉田淑子,岡部素典,小池千加,周凱旋
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 20100328-30
• [Presentation] ヒト羊膜由来細胞 (2010)
  • Author(s): 岡部素典,吉田淑子,小池千加,津野宏彰,二階堂敏雄
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 20100328-30
• [Presentation] 不死化羊膜間葉系細胞およびヒアルロン酸オリゴ糖(HA)投与による脊髄損傷治癒効果の検討 (2010)
  • Author(s): 吉田淑子,岡部素典,吉田一晴,小池千加,周凱旋, Moustaf Fathy,京哲,清野透,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] ヒト乾燥羊膜は凍結乾燥ヒト羊膜より有効 (2010)
  • Author(s): 岡部素典,吉田淑子,小池千加,齋藤滋,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] 羊膜由来細胞のStemnessの度合いの解析 (2010)
  • Author(s): 小池千加,周凱旋,吉田淑子,岡部素典,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] ヒト羊膜間葉系細胞の骨再生医療への応用 (2010)
  • Author(s): 津野宏彰,吉田淑子,野上真紀子,小池千加,岡部素典,能登善弘,野口誠,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] OCT3/4発現の誘導はヒト羊膜問葉系細胞を未分化状態へ励起し心筋分化能を改善する (2010)
  • Author(s): 名倉里織*,大高慎吾,小池千加,岡部素典,吉田淑子,柳堅徳,深原一晃,三崎拓郎,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] Nanog陽性羊膜間葉系幹細胞の選択的分離と心筋分化誘導 (2010)
  • Author(s): 大高慎吾*,名倉里織,小池千加,岡部素典,吉田淑子,柳堅徳,三崎拓郎,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] 不死化ヒト羊膜上皮細胞の同定と解析 (2010)
  • Author(s): 周凱旋*,小池千加,吉田淑子,岡部素典,名倉里織,能登善弘,竹田裕治,京哲,清野透,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] マウス羊膜幹細胞の分化能 (2009)
  • Author(s): 吉田淑子, Teng Zan,周凱旋,岡部素典,小池千加,米田徳子,野上真紀子,樋口収,二階堂敏雄
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20090305-06
• [Presentation] ヒト羊膜間葉系幹細胞の顎骨再生医療への応用についての検討 (2009)
  • Author(s): 津野宏彰,吉田淑子,野上真紀子,小池千加,岡部素典,能登善弘,野口誠,二階堂敏雄
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20090305-06
• [Presentation] 羊膜間葉系幹細胞(HAM)を用いた軟骨細胞分化誘導 (2009)
  • Author(s): 野上真紀子*,津野宏彰,小池千加,岡部素典,吉田淑子,木村友厚,二階堂敏雄
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20090305-06
• [Book] 改訂第3版遺伝子工学実験ノート (2010)
  • Author(s): 小池千加
  • Total Pages: 上162, 168-173,下126-137
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.med.u-toyama.ac.jp/saiseiigaku/index.html