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2011 Fiscal Year Final Research Report

Shear stress-dependent vasospasm induced by lysophosphatidic acid

Research Project

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Project/Area Number 21590240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionShowa University

Principal Investigator

OHATA Hisayuki  昭和大学, 薬学部, 准教授 (00119166)

Project Period (FY) 2009 – 2011
Keywords血管 / 内皮細胞 / 平滑筋細胞 / リゾリン脂質 / 収縮弛緩反応
Research Abstract

We have previously shown that lysophosphatidic acid(LPA), a bioactive plasma lysophospholipid, markedly accelerates shear stress. induced Ca^<2+> responses in cultured vascular endothelial cells(ECs). This study aimed to demonstrate the impact of LPA and luminal shear stress on vasomotor regulation in the isolated rat mesenteric artery(MA) using a videomicroscopic technique. Although the addition of LPA to the perfusate in a concentration range of 0.03.0.3. M had no significant effect on the basal MA tone, LPA in a similar concentration range led to increased phenylephrineinduced MA contraction and reduced acetylcholine-induced MA relaxation under physiological shear conditions. These vasomodulatory actions of LPA, which vanished upon removal of ECs, were positively dependent on luminal shear stress levels and were markedly inhibited by the LPA receptor antagonist Ki16425, the cyclooxygenase inhibitor indomethacin, and the thromboxane A_2 receptor antagonist SQ29548. These data thus suggest that LPA can modify the agonistinduced vasomotor responses in MAs in a shear stress. dependent manner. This effect of LPA was mediated through ECs, the LPA receptor, and cyclooxygenase/thromboxane A_2 signaling.

  • Research Products

    (2 results)

All 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Lysophosphatidic acid induces shear stress-dependent Ca^<2+> influx in mouse aortic endothelial cells in situ2011

    • Author(s)
      H. Ohata, H. Yamada, K. Momose
    • Journal Title

      Experimental Physiol

      Volume: 96 Pages: 468-475

    • Peer Reviewed
  • [Journal Article] Shear Stress-dependent Effects of Lysophosphatidic Acid on Agonist-induced Vasomotor Responses in Rat Mesenteric Artery2011

    • Author(s)
      K. Shibata, T. Miyazaki, H. Ohata and K. Honda
    • Journal Title

      J Cardiovascul Pharmacol

      Volume: 57 Pages: 604-610

    • Peer Reviewed

URL: 

Published: 2013-07-31  

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