2011 Fiscal Year Final Research Report
On the mechanism of modal regulation of cardiovascular Ca2+entry channel TRPC6
| Project/Area Number |
21590246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Fukuoka University |
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Principal Investigator |
INOUE Ryuji 福岡大学, 医学部, 教授 (30232573)
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| Project Period (FY) |
2009 – 2011
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| Keywords | Ca^<2+>チャネル / TRP蛋白質 / リン酸化 / 受容体・機械刺激 / 形質転換 / 活性化モード / 細胞骨格 / 蛋白質間相互作用 |
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| Research Abstract |
The canonical transient receptor potential protein 6(TRPC6) is a Ca^<2+> entry channel activated by synergistic operation of receptor-mediated and mechanosentive signaling pathways. Its excessive activity contributes to the pathogenesis of cardiovascular diseases. The results of the present study suggest that the synergy between receptor and mechanical stimulations on TRPC6 channel activation likely occurs through physical interaction and functional coupling between TRPC6 channel N-terminal ankyrin-like domains and actin cytoskeleton. Further, this synergy is exaggerated by pathogenic mutations in amino acid residues adjacent to these domains, and counteracted byprotein kinase G-mediated phosphorylation of TRPC6 N-terminus, by strengthening and disrupting the N-terminus domain-actin cytoskeleton interaction, respectively.
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[Journal Article] Cilostazol Suppresses Angiotensin II-Induced Vasoconstriction via Protein Kinase A-Mediated Phosphorylation of the Transient Receptor Potential Canonical 6 Channel2011
Author(s)
Nishioka K, Nishida M, Ariyoshi M, Jian Z, Saiki S, Hirano M, Nakaya M, Sato Y, Kita S, Iwamoto T, Hirano K, Inoue R, Kurose H.
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 31
Pages: 2278-2286
DOI
Peer Reviewed
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[Journal Article] Dualsignaling pathways of arterial constriction by extracellular UTP in the rat2011
Author(s)
Sugihara M, Morita H, Matsuda M, Umebayashi H, Kajioka S, Ito S, Nishida M, Inoue R, Futatsuki T, Yamazaki J, Mori Y, Inoue R, Ito Y, Abe K, Hirata M.
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Journal Title
J Pharmacol Sci
Volume: 115(3)
Pages: 293-308
DOI
Peer Reviewed
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[Journal Article] Ca^<2+> Influx and protein scaffolding via TRPC3 sustain PKCβ and ERK activation in B cells2010
Author(s)
Numaga T, Nishida M, Kiyonaka S, Kato K, Katano M, Mori E, Kurosaki T, Inoue R, Hikida M, Putney JWJr, Mori Y.
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Journal Title
J Cell Sci
Volume: 123(6)
Pages: 927-938
DOI
Peer Reviewed
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[Journal Article] Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via PLC/diacylglycerol and PLA2/ω-hydroxylase/20-HETE pathways2009
Author(s)
Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salmonsson M, Morita H, Kawarabayashi Y, Mori M, Mori Y, Ito Y.
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Journal Title
Circ Res
Volume: 104
Pages: 1399-1409
DOI
Peer Reviewed
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