2011 Fiscal Year Final Research Report
Elucidation of circadian clock machinery by development of an in vivo multi-gene silencing technique
| Project/Area Number |
21590264
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Research Abstract |
We have previously generated knockdown mice expressing shRNA of candidate genes involved in circadian rhythm regulation using a lentiviral vector. In this study, we further developed a technique to express two different shRNAs in vivo and successfully generated double knockdown mice. However, these mice exhibited significant individual differences in the knockdown efficiency and silencing effects among generations. Therefore, we next examined double knockout or cell type-specific knockout mice. Our data demonstrated abnormal circadian behavioral rhythms in different knockout mice strains and suggested involvements of novel candidate genes in the circadian clock machinery.
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[Journal Article] In vivo diagnostic imaging using micro-CT : sequential and comparative evaluation of rodent models for hepatic/brain ischemia and stroke2012
Author(s)
Hayasaka N, Nagai N, Kawao N, Niwa A, Yoshioka Y, Mori Y, Shigeta H, Kashiwagi N, Miyazawa M, Satou T, Higashino H, Matsuo O, Murakami T
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Journal Title
PLoS One
Volume: 7
Pages: e32342
Peer Reviewed
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[Journal Article] Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in rgs16 knockdown mice2011
Author(s)
Hayasaka N, Aoki K, Kinoshita S, Yamaguchi S, Wakefield JK, Tsuji-Kawahara S, Horikawa K, Ikegami H, Wakana S, Murakami T, Ramabhadran R, Miyazawa M, Shibata S
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Journal Title
PLoS One
Volume: 6
Pages: e17655
Peer Reviewed
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