2011 Fiscal Year Final Research Report
Analysis of intracellular localization of clock protein by means of bioluminescence imaging technique
| Project/Area Number |
21590266
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NAKAJIMA Yoshihiro 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (10291080)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生物時計 |
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| Research Abstract |
To visualize intracellular localization of clock protein by means of bioluminescence imaging technique, we have developed an enhanced green-emitting luciferase(ELuc) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase(FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visualization of gene expression with high temporal resolution at the single-cell level. Moreover, we successfully imaged the nucleocytoplasmic shuttling of clock protein(mPER2 and mCRY2) by fusing ELuc at the intracellular level.
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