Functional role of a p53 mRNA export factor, GANP, in the occurrence of sporadic breast cancers

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Kumamoto University
• Principal Investigator: KUWAHARA Kazuhiko 熊本大学, 大学院・生命科学研究部, 准教授 (10263469)
• Project Period (FY): 2009 – 2011
• Keywords: 非遺伝性乳癌 / mRNA核外輸送

Research Abstract

Mammalian cells, unlike Saccharomyces cerevisiae, have the mechanism of selective mRNA export. GANP is involved in mRNA export in mammalian cells, and mammary-specific ganp-deficient mice developed mammary gland tumors with lung metastases. Moreover, decreased expression of GANP induced DNA-damage responses leading to the impaired formation of mammary glands. As this abnormality is similar to that observed in Brca1-deficient mice, GANP expression might be associated with the suppression of sporadic breast cancers.

Research Products

• [Journal Article] Dynamic appearance of antigenic epitopes effective for viral neutralization during membrane fusion initiated by interactions between HIV-1 envelope proteins and CD4/CXCR4 (2012)
  • Author(s): Toda, T., Kuwahara, K., Kondo, N., Matsuda, Z., Maeda, Y., Maeda, K. & Sakaguchi, N.
  • Journal Title: Immunobiology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP (2012)
  • Author(s): Phimsen, S., Kuwahara, K., Nakaya, T., Ohta, K., Suda, T., Kitabatake, M., Rezano, A., Vaeteewoottacharn, K., Okada, S., Tone, S. & Sakaguchi, N.
  • Journal Title: Apoptosis Volume: 17 Pages: 679-690
  • Peer Reviewed
• [Journal Article] Molecular mechanism of immunoglobulin V-region diversification regulated by transcription and RNA metabolism in antigen-driven B-cells (2011)
  • Author(s): Sakaguchi, N., Maeda, K. & Kuwahara, K.
  • Journal Title: Scand. J. Immu Volume: 73 Pages: 523-526
  • Peer Reviewed
• [Journal Article] A novel serum carbohydrate marker on MUC5AC : values for diagnostic and prognostic indicators for cholangiocarcinoma (2011)
  • Author(s): Silsirivanit, A., Araki, N., Wongkham, C., Pairojkul, C., Narimatsu, Y., Kuwahara, K., Narimatsu, H., Wongkham, S. & Sakaguchi, N.
  • Journal Title: Cancer Volume: 117 Pages: 3393-3403
  • Peer Reviewed
• [Journal Article] Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression (2010)
  • Author(s): Nakaya, T., Kuwahara, K., Ohta, K., Kitabatake, M., Toda, T., Kondo, E. & Sakaguchi, N.
  • Journal Title: J. Immunol Volume: 185 Pages: 5180-5187
  • Peer Reviewed
• [Journal Article] Persistent expression of the full genome of hepatitis C virus in B cells induces spontaneous development of B-cell lymphomas in vivo (2010)
  • Author(s): Kasama, Y., Sekiguchi, S., Saito, M., Tanaka, K., Satoh, M., Kuwahara, K., Sakaguchi, N., Takeya, M., Hiasa, Y., Kohara, M. & Tsukiyama-Kohara K.
  • Journal Title: Blood Volume: 116 Pages: 4926-4933
  • Peer Reviewed
• [Journal Article] Germinal center-associated nuclear protein(GANP) is involved in mRNA export of Shugoshin-1 required for centromere cohesion and in sister-chromatid exchange (2010)
  • Author(s): Okamoto, N., Kuwahara, K., Ohta, K., Kitabatake, M., Takagi, K., Mizuta, H., Kondo, E. & Sakaguchi, N.
  • Journal Title: Genes Cells Volume: 15 Pages: 471-484
  • Peer Reviewed
• [Journal Article] Decreased expression of germinal center-associated nuclear protein is involved in chromosomal instability in malignant gliomas (2009)
  • Author(s): Ohta, K., Kuwahara, K., Zhang, Z., Makino, K., Komohara, Y., Nakamura, H., Kuratsu, J. & Sakaguchi, N.
  • Journal Title: Cancer Sci Volume: 100 Pages: 2069-2076
  • Peer Reviewed
• [Journal Article] GANP, involved in immunoglobulin V-region diversification, is co-expressed with AID in cholangiocarcinomas associated with long-term inflammation caused by liver fluke infestation (2009)
  • Author(s): Chan-on, W., Kuwahara, K., Kobayashi, N., Ohta, K., Shimasaki, T., Banchob, S., Leelayuwat, C. & Sakaguchi, N.
  • Journal Title: Int. J. Oncol Volume: 35 Pages: 287-195
  • Peer Reviewed
• [Journal Article] Generation of high affinity monoclonal antibodies for the prevention of HIV infection. Recent Pat (2009)
  • Author(s): Sakaguchi, N., Toda, T., Nakaya, T., Kitabatake, M., Maeda, K. & Kuwahara, K.
  • Journal Title: DNA Gene Seq Volume: 3 Pages: 88-95
  • Peer Reviewed
• [Journal Article] GANP suppresses the arginine methyltransferase PRMT5 regulating IL-4-mediated STAT6-signaling to IgE production in B cells (2009)
  • Author(s): Igarashi, H., Kuwahara, K., Yoshida, M., Xing, Y., Maeda, K., Nakajima, K. & Sakaguchi, N.
  • Journal Title: Mol. Immunol Volume: 46 Pages: 1031-1041
  • Peer Reviewed
• [Presentation] Lynを介するシグナルターゲットであるGANPは末梢リンパ組織における高親和性抗体産生B細胞の維持に重要である (2011)
  • Author(s): 桑原一彦、Suchada Phimsen、竹森利忠、渡辺武、阪口薫雄
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-11-27
• [Presentation] 乳腺特異的GANP欠損マウスにおける乳管形成障害と相関する高頻度乳癌発症 (2011)
  • Author(s): 桑原一彦、阪口薫雄
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] Role of GANP in prevention of cancer development (2010)
  • Author(s): Kuwahara, K. and Sakaguchi, N.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] GANP plays a critical role in activation of B cell proliferation as a Lyn-mediated signaling target (2010)
  • Author(s): Kuwahara, K., Nakaya, T., Watanabe, T. and Sakaguchi, N.
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-08-24
• [Presentation] Role of GANP family members in the maturation of germinal center B cells (2009)
  • Author(s): Kuwahara, K. and Sakaguchi, N.
  • Organizer: 第39回日本免疫学会学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-04
• [Presentation] Critical role of GANP in nuclear export of mRNA involved in mammalian cell division (2009)
  • Author(s): Kuwahara, K. and Sakaguchi, N.
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-03
• [Book] 細胞死実験プロトコール (2011)
  • Author(s): 桑原一彦、Suchada Phimsen、阪口薫雄
  • Total Pages: 65-69
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.k-immu.jp/ja/