Analysis of function of FGF21 on metabolism

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590343
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Fukushima Medical University
• Principal Investigator: YAMAMOTO Masaya 福島県立医科大学, 医学部, 准教授 (20446115)
• Project Period (FY): 2009 – 2011
• Keywords: 線維芽細胞増殖因子 / 情報伝達 / 代謝調節

Research Abstract

βKlotho was focused on, due to the importance to FGF21 function
According to the analysis of the HEK293 cell expressingβKlotho stably
1)βKlotho shifts the molecule size and amount along with the glucose concentration.
2) High glucose treatment leadsβKlotho molecule size shift and amount in 30 min.
According to differentiated caco2 cell, which induce endogenousβKlotho expression
1) High glucose condition selectively enhances FGF21 signaling
2)βKlotho distributes mainly in cytosol as a vesicular like pattern.

Research Products

• [Journal Article] Mitomycin C treatment significantly reduces central damage of islets in culture (2012)
  • Author(s): Saito T, Saito T, Sato Y, Ise K, Anazawa T, Oshibe I, Haga J, Yamamoto M, Waguri S, Gotoh M
  • Journal Title: Pancreas Volume: 41 Pages: 245-252
• [Journal Article] Cathepsin L is highly expressed in gastrointestinal stromal tumors (2011)
  • Author(s): Miyamoto K, Iwadate M, Yanagisawa Y, Ito E, Imai J, Yamamoto M, Sawada N, Saito M, SuzukiS, Nakamura I, Ohki S, Saze Z, KogureM, Gotoh M, Omicronbara K, Ohira H, Tasaki K, Abe M, Goshima N, Watanabe S, Waguri S, Takenoshita S
  • Journal Title: Int J Oncol Volume: 39 Pages: 1109-1115
• [Journal Article] Mechanism of statin-induced contractile dysfunction in rat cultured skeletal myofibers (2010)
  • Author(s): Tanaka S, Sakamoto K, Yamamoto M, Mizuno A, Ono T, Waguri S, Kimura J
  • Journal Title: J Pharmacol Sci Volume: 114 Pages: 454-463
• [Journal Article] (2009)
  • Author(s): Chen MZ, Zhu X, Sun HQ, Mao YS, Wei Y, Yamamoto M, Yin HL
  • Journal Title: J Biol Chem Volume: 284 Pages: 23743-23753
• [Presentation] 培養細胞を用いたβklothoの動態・局在解析 (2011)
  • Author(s): 山本雅哉、佐々木理、和栗聡
  • Organizer: 第57回解剖学会東北・北海道連合支部学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-09-10
• [Presentation] Atg3欠損マウス線維芽細胞株におけるオメガソームマーカーDFCP1の局在解析 (2010)
  • Author(s): 亀高愛、山田茜、澤田直樹、山本雅哉、曽友深、小松雅明、和栗聡
  • Organizer: 第56回東北・北海道連合支部学術集会
  • Place of Presentation: 旭川
  • Year and Date: 2010-09-25
• [Presentation] Atg3欠損マウス線維芽細胞株で見られたオートファゴソーム様構造の形態学的解析 (2010)
  • Author(s): 山田茜、亀高愛、山本雅哉、曽友深、小松雅明、和栗聡
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] ラット皮膚創傷治癒過程におけるMAP1LC3の免疫組織学的動態解析 (2010)
  • Author(s): 浅井笑子、山本雅哉、上田和毅、和栗聡
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] Distribution analysis ofβKlotho in liver and pancreas (2009)
  • Author(s): Ymamoto M, Waguri S
  • Organizer: アメリカ細胞生物学会
  • Place of Presentation: サンディエゴ
  • Year and Date: 2009-12-08
• [Presentation] マウス組織におけるβKlothoの発現分布解析 (2009)
  • Author(s): 山本雅哉
  • Organizer: 第55回東北・北海道連合支部学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2009-09-26
• [Presentation] Hypertonic Stress Increases Phosphatidylinositol 4, 5-Bisphosphate Levels by Activating PIP5KIβ (2009)
  • Author(s): Yamamoto M, Yin H
  • Organizer: International symposium on Autophagy and Cell Death
  • Place of Presentation: 東京
  • Year and Date: 2009-07-04