2011 Fiscal Year Final Research Report

Identification of placenta specific small RNA

Research Project

Project/Area Number	21590364
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Human genetics
Research Institution	National Research Institute for Child Health and Development
Principal Investigator	HATA Kenichiro 独立行政法人国立成育医療研究センター, 周産期病態研究部, 部長 (60360335)
Project Period (FY)	2009 – 2011
Keywords	胎盤 / small RNA / エピジェネティクス / TS細胞 / DNAメチル化
Research Abstract	We prepared total RNA from several trophoblast stem cell lines under FGF4 contained medium condition to keep undifferentiated state. Then collected total RNA were purified by column and polyacrylamide gel electrophoresis to pick up highly purified 20-40 mer small RNA fractions. These purified small RNA molecules were ligated with special adaptors and then reveres transcription and PCR were done with adapter-ligated RNA molecules to make the small RNA libraries of torophoblast stem cell lines. The libraries were sequenced and analyzed by small RNA micro arrays. Sequencing and microarray data showed some miRNA families were expressed in both torophoblast stem cells and placental tissues. A part of miRNAs are derived from a miRNA cluster in extraembryonic tissues of E9.5 specifically. Our finding suggests that the miRNAs could be regulated uniquely in mouse development.

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