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2011 Fiscal Year Final Research Report

Application of dual-color CISH for pathological diagnosis and extraction of prognostic factors in bone and soft tissue sarcomas

Research Project

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Project/Area Number 21590386
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTokyo Medical University (2011)
Teikyo University (2009-2010)

Principal Investigator

MOTOI Tooru  東京医科大学, 医学部, 兼任講師 (50291315)

Co-Investigator(Kenkyū-buntansha) KUMAGAI Arisa  帝京大学, 医学部, 教務職員 (50596963)
Co-Investigator(Renkei-kenkyūsha) IMAMURA Tetsuo  帝京大学, 医学部, 教授 (40010566)
Project Period (FY) 2009 – 2011
KeywordsCISH法 / 肉腫 / 分子病理診断 / 染色体相互転座 / 融合遺伝子 / 遺伝子増幅
Research Abstract

Accurate pathological diagnosis of sarcoma is indispensable for appropriate therapy. However, rarity and wide variety of histological types make tumor diagnosis difficult. In this study, a detection method which enables simultaneous visualization of morphology and cytogenetic abnormalities under a light microscope is established and verified. The new method is applicable for routine pathological diagnosis without using special equipment for molecular biology. Therefore diagnostic accuracy may be improved by the present method.

  • Research Products

    (27 results)

All 2012 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 8 results) Presentation (14 results) Book (2 results)

  • [Journal Article] MDM2 and CDK4 immunohistochemical coexpression in high-grade osteosarcoma : correlation with a dedifferentiated subtype2012

    • Author(s)
      Yoshida A, Ushiku T, Motoi T, 他4名
    • Journal Title

      Am J Surg Pathol

      Volume: 36(3) Pages: 423-31

    • DOI

      DOI:10.1097/PAS.0b013e31824230d0

    • Peer Reviewed
  • [Journal Article] Inhibin-αand synaptophysin immunoreactivity in synovial sarcoma with granular cell features2012

    • Author(s)
      Setsu N, Kohashi K, Endo M, Yamamoto H, Ohishi Y, Sueyoshi K, Iwamoto Y, Tsuneyoshi M, Motoi T, Kumagai A, Oda Y
    • Journal Title

      Hum Pathol

      Volume: 43(6) Pages: 850-7

    • DOI

      doi:10.1016/j.humpath.2011.07.012

    • Peer Reviewed
  • [Journal Article] Identification of a novel, recurrent HEY1-NCOA2 fusion in mesenchymalchondrosarcoma based on a genome-wide screen of exon-level expression data2012

    • Author(s)
      Wang L, Motoi T, 他12名
    • Journal Title

      Genes Chromosomes Cancer

      Volume: 51(2) Pages: 127-39

    • DOI

      DOI:10.1002/gcc.20937

    • Peer Reviewed
  • [Journal Article] 軟部腫瘍の細胞遺伝学的診断法,軟部腫瘍I-診断と研究の進歩-,今日の軟部腫瘍の治療と分子生物学2012

    • Author(s)
      元井亨、加藤生真
    • Journal Title

      病理と臨床

      Volume: 30巻2号 Pages: 127-135

    • URL

      http://www.bunkodo.co.jp

  • [Journal Article] Osteomalacia caused by skull base tumors : report of 2 cases2011

    • Author(s)
      Uno T, Kawai K, Kunii N, Fukumoto S, Shibahara J, Motoi T, Saito N
    • Journal Title

      Neurosurgery

      Volume: 69(1) Pages: E239-44

    • DOI

      doi:10.1227/NEU.0b013e31821867f7

    • Peer Reviewed
  • [Journal Article] Well-differentiated liposarcoma with low-grade osteosarcomatous component : an underrecognized variant2010

    • Author(s)
      Yoshida A, Ushiku T, Motoi T, 他3名
    • Journal Title

      Am J Surg Pathol

      Volume: 34(9) Pages: 1361-6

    • DOI

      doi:10.1097/PAS.0b013e3181ebcc45

    • Peer Reviewed
  • [Journal Article] Detection of SYT and EWS gene rearrangements by dual-color break-apart CISH in liquidbased cytology samples of synovial sarcoma and Ewing sarcoma/primitive neuroectodermal tumor2010

    • Author(s)
      Kumagai A, Motoi T, 他3名
    • Journal Title

      Am J Clin Pathol

      Volume: 134(2) Pages: 323-31

    • DOI

      doi:10.1309/AJCPTLSM15XKPDDU

    • Peer Reviewed
  • [Journal Article] Immunohistochemical analysis of MDM2 and CDK4 distinguishes low-grade osteosarcoma from benign mimics2010

    • Author(s)
      Yoshida A, Ushiku T, Motoi T, 他4名
    • Journal Title

      Mod Pathol

      Volume: 23(9) Pages: 1279-88

    • DOI

      doi:10.1038/modpathol.2010.124

    • Peer Reviewed
  • [Journal Article] Diagnostic utility of dual-color break-apart chromogenic in situ hybridization for the detection of rearranged SS18 in formalin-fixed, paraffin-embedded synovialsarcoma2010

    • Author(s)
      Motoi T, Kumagai A, 他3名
    • Journal Title

      Hum Pathol

      Volume: 41(10) Pages: 1397-404

    • DOI

      doi:10.1016/j.humpath.2010.02.009

    • Peer Reviewed
  • [Journal Article] 骨・軟部腫瘍特異的融合遺伝子,病理形態学キーワード2010

    • Author(s)
      元井亨, 熊谷有紗
    • Journal Title

      病理と臨床

      Volume: 28 Pages: 368-370

    • URL

      http://www.bunkodo.co.jp

  • [Journal Article] Application of CISH Technology on Diagnosis of Translocation-Associated Soft Tissue Sarcomas2009

    • Author(s)
      Motoi T, Kumagai A
    • Journal Title

      Connection(DAKO)

      Volume: 13 Pages: 83-91

    • URL

      http://www.dako.jp/index/knowledgecenter/kc_publications/kc_publications_connection/kc_publications_connection13-2.htm

  • [Presentation] 確定診断が困難な軟部原発粘液性腫瘍3症例の捺印細胞像の検討2012

    • Author(s)
      元井亨, 他9名
    • Organizer
      第53回日本臨床細胞学会総会春季大会
    • Place of Presentation
      千葉市
    • Year and Date
      2012-06-02
  • [Presentation] 著明なメラニン産生を示した軟部原発Perivascular epithelioid cell tumorの捺印細胞像及びCISH法を用いた細胞遺伝学解析2012

    • Author(s)
      加藤生真, 元井亨, 他8名
    • Organizer
      第53回日本臨床細胞学会総会春季大
    • Place of Presentation
      千葉市
    • Year and Date
      2012-06-02
  • [Presentation] TFE3 Gene Rearrangement Status Is Heterogeneous in Alveolar Soft Part Sarcomas : A Study by Dual-Color Chromogenic In Situ Hybridization on Formalin-Fixed Paraffin-Embedded Samples2012

    • Author(s)
      Motoi T, 他6名
    • Organizer
      101^<st> Annual meeting, United States and Canadian Academy of Pathology
    • Place of Presentation
      カナダ,バンクーバー
    • Year and Date
      2012-03-19
  • [Presentation] MicroRNA 200a is Related to Epithelial Differentiation of Synovial Sarcoma2011

    • Author(s)
      Motoi T, Kumagai A, Yoshida A, Imamura T, Fukusato T
    • Organizer
      100th Annual Meeting, United States and Canadian Academy of Pathology
    • Place of Presentation
      アメリカ合衆国,サンアントニオ
    • Year and Date
      2011-03-01
  • [Presentation] MDM2-p53 Pathway Status in Clinical Samples of Well-Differentiated and Dedifferentiated Liposarcoma2011

    • Author(s)
      Kumagai A, Motoi T, 他4名
    • Organizer
      100th Annual Meeting, States and Canadian Academy of Pathology
    • Place of Presentation
      アメリカ合衆国,サンアントニオ
    • Year and Date
      2011-02-28
  • [Presentation] Identification of a Novel, Recurrent HEY1-NCOA2 Fusion in Mesenchymal Chondrosarcoma Based on a Genome-Wide Screen of Exon-Level Expression Data2011

    • Author(s)
      Wang L, Motoi T, 他11名
    • Organizer
      100th Annual Meeting, United States and Canadian Academy of Pathology
    • Place of Presentation
      米国、サンアントニオ
    • Year and Date
      2011-02-28
  • [Presentation] Diagnostic Utility of MDM2 Gene Amplification Detected by Dual-Color Chromogenic In Situ Hybridization on Liquid Based-Cytology of Well Differentiuated Liposarcoma2010

    • Author(s)
      Motoi T, Kumagai A, 他4名
    • Organizer
      17th International Congress of Cytology
    • Place of Presentation
      イギリス,エディンバラ
    • Year and Date
      20100518-19
  • [Presentation] Cytogenetic Analyses of MDM2 status by Dual-Color Chromogenic In Situ Hybridization in Liquid-Based Cytology Samples of Dedifferentiated Liposarcoma and Pleomorphic Malignant Fibrous Histiocytoma2010

    • Author(s)
      Kumagai A, Motoi T, 他4名
    • Organizer
      17th International Congress of Cytology
    • Place of Presentation
      イギリス、エディンバラ
    • Year and Date
      20100518-19
  • [Presentation] 光学顕微鏡下での骨軟部肉腫の細胞遺伝学的異常の解析、LBC標本上のCISH法を用いて2010

    • Author(s)
      元井亨
    • Organizer
      第49回日本臨床細胞学会秋期大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-11-22
  • [Presentation] Immunohistochemical and Cytogenetic Analyses of FGF23 and Chondrogenic and Osteogenic Transcription Factor Expression in Phosphaturic Mesenchymal tumor2010

    • Author(s)
      Kumagai A, Motoi T, 他6名
    • Organizer
      99th Annual meeting, United States Canadian Academy of Pathology
    • Place of Presentation
      米国,ワシントン
    • Year and Date
      2010-03-22
  • [Presentation] Cytogenetic and Immunohistochemical Analyses of c-MET in Peripheral Nerve Sheath Tumors, 99th Annual meeting2010

    • Author(s)
      Motoi T, Kumagai A, 他4名
    • Organizer
      United States Canadian Academy of Pathology
    • Place of Presentation
      米国,ワシントン
    • Year and Date
      2010-03-22
  • [Presentation] Cytological and Molecular Cytogenetic Feature of Phosphaturic Mesenchymal Tumor : A case report2009

    • Author(s)
      Kumagai A, Motoi T, 他7名
    • Organizer
      35th European Congress of Cytopathology
    • Place of Presentation
      ポルトガル,リスボン
    • Year and Date
      2009-09-28
  • [Presentation] Detection of SYT and EWS Gene Rearrangements by Dual-Color Break-apart CISH on Liquid-based Cytology Samples of Synovial Sarcoma and Ewing Sarcoma2009

    • Author(s)
      Motoi T, Kumagai A, 他4名
    • Organizer
      35th European Congress of Cytopathology
    • Place of Presentation
      ポルトガル,リスボン
    • Year and Date
      2009-09-28
  • [Presentation] 滑膜肉腫における分子病理学的検討2009

    • Author(s)
      元井亨
    • Organizer
      第7回関東骨軟部腫瘍の基礎を語る会
    • Place of Presentation
      東京
    • Year and Date
      2009-04-11
  • [Book] 別冊日本臨床新領域別症候群シリーズ肝・胆道系症候群(第二版),肝外胆管領域の脂肪性腫瘍2011

    • Author(s)
      元井亨, 熊谷有紗、福里利夫
    • Total Pages
      41-44
    • Publisher
      日本臨床社
  • [Book] 腫瘍病理鑑別診断アトラス軟部腫瘍,滑膜肉腫2011

    • Author(s)
      元井亨, 熊谷有紗
    • Total Pages
      116-125
    • Publisher
      文光堂

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Published: 2013-07-31  

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