Expression mechanism and pathological significance of Pentraxin 3 in macrophages and neutrophils.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590397
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Niigata University
• Principal Investigator: NAITO Makoto 新潟大学, 医歯学系, 教授 (30045786)
• Co-Investigator(Kenkyū-buntansha): OHASHI Riuko 新潟大学, 医歯学系, 助教 (20447600)
• Project Period (FY): 2009 – 2011
• Keywords: マクロファージ / 好中球 / Pentraxin 3 / 潰瘍性大腸炎

Research Abstract

Neutrophils in colonic mucosal tissue of patients with ulcerative colitis were main cellular source of Pentraxin 3(PTX3) protein, suggesting that PTX3 protein may contribute to cell-mediated immune defense in inflamed colon tissue of patients with ulcerative colitis. PTX3 protein was found to be present together with lactoferrin^+-specific granules localized in neutrophils. Upon IL-8 stimulation, PTX3 is released from PMNs and localized in Neutrophil Extracellular Traps(NETs) formed by extruded DNA. Macrophages showed granular positivity of PTX3. Secretion of PTX3 from stimulated neutrophils and macrophages was confirmed in culture. Binding of PTX3 on NETs appeared to be a defense mechanism against pathogens.

Research Products

• [Journal Article] Long pentraxin 3(PTX3) expression and release by neutrophils in vitro and in ulcerative colitis (2011)
  • Author(s): Savchenko AS, Inoue A, Ohashi R, Jiang S, Hasegawa G, Tanaka T, Hamakubo T, Kodama T, Aoyagi Y, Ushiki T, Naito M
  • Journal Title: Pathol Int Volume: 61 Pages: 290-297
  • Peer Reviewed
• [Journal Article] The proteomic profile of circulating pentraxin 3(PTX3) complex in sepsis demonstrates the interaction with azurocidin 1 and other components of neutrophil extracellular traps (2011)
  • Author(s): Daigo K, Yamaguchi N, Kawamura T, Matsubara K, Jiang S, Ohashi R, Sudou Y, Kodama T, Naito M, Inoue K, Hamakubo T
  • Journal Title: J Biol Chem Volume: 286(1) Pages: 674-86
  • Peer Reviewed
• [Journal Article] 好中球とPTX3 (2010)
  • Author(s): 内藤眞、AS Savchenko、井上聡
  • Journal Title: 生物資料分析 Volume: 33(4) Pages: 329-338
• [Presentation] 好中球とマクロファージにおけるPTX3の局在と分泌 (2011)
  • Author(s): 松原浩一、大橋瑠子、S. Alexander, 他
  • Organizer: 第51回日本リンパ網内系学会総会
  • Place of Presentation: 福岡市国際会議場
  • Year and Date: 20110630-0702
• [Presentation] Different response of cells to hypoxia in expression of PTX3 in human atherosclerosis and in vitro (2011)
  • Author(s): Alexander AS、井上聡、大橋瑠子, 他
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 20110428-30
• [Presentation] Differences in response of macrophages and neutrophils to hypoxia in expression of pentraxin 3(PTX3) in human atherosclerosis and in vitro (2011)
  • Author(s): Alexander AS, Naito M, et al.
  • Organizer: Leukocyte in Cardiovascular Disease Conference
  • Place of Presentation: Geneva, Switzer-land
  • Year and Date: 20110127-28
• [Presentation] Pentraxin 3(PTX3) is a novel predictive marker for acute coronary events. (2010)
  • Author(s): Alexander AS, Naito M, et al.
  • Organizer: International Symposium "New methods of acute coronary syndrome diagnosis and treatment"
  • Place of Presentation: St. Petersburg, Russia
  • Year and Date: 20100915-18
• [Presentation] The penomenon of hypoxia-induced expression of long pentraxin 3(PTX3) in human atherosclerosis (2010)
  • Author(s): Alexander AS,大橋瑠子,井上聡,姜淑英,長谷川剛,内藤眞
  • Organizer: 第50回日本リンパ網内系学会総会
  • Place of Presentation: 新潟市朱鷺メッセ
  • Year and Date: 20100618-19
• [Presentation] Long pentraxin 3(PTX3) is stored and potentially released from neutrophils in vitro and in human atherosclerosis lesions (2010)
  • Author(s): Alexander AS, Naito M, et al.
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20100408-10
• [Presentation] 潰瘍性大腸炎におけるPentraxin 3の発現 (2009)
  • Author(s): 井上聡、姜淑英、長谷川剛、Alexander AS、内藤眞
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都市京都国際会議場
  • Year and Date: 20090501-03