2011 Fiscal Year Final Research Report
Role of gap-junctional coupling between cardiomyocytes and stromal cells in genesis of cardiac arrhythmias
| Project/Area Number |
21590420
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TANAKA Hideo 京都府立医科大学, 大学院・医学研究科, 准教授 (60236619)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 線維芽細胞 / 心筋梗塞 / ギャップ結合 / 不整脈 / 肉芽組織 |
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| Research Abstract |
During the healing phase of myocardial infarct, proliferation of endothelial cells and fibroblasts constitute granulation tissue to replace the infarct resion. Recent reports have confirmed that stromal cells, especially fibroblasts, coupled electrically with cardiomyocytes via gap junction proteins connexins, provide an important basis for cardiac arrhythmias.However, it is unresoloved whether or not stromal cells at the border zone of myocardial infarct really contribute to the arrhythmogenesis. To address this, this project was conducted by using an infarct model of conditional knockout mice specific for Cx43 in the Fsp-1 proteins. Continuous ECG recording in combination with histological analysis was conducted in the Fsp-Cre/Cx43 loxP mice. It was found that the heart devoid of Cx43 in the fibroblasts and endothelial cells failed to attenuate ventricular arrhythmias after infarction. However, formation of granulation tissue showed a significant retardation in the Cx43-KO mice after infarction as compared with wild type mice. The results indicate that Cx43 contributes to formation of granulation tissue in the infarct heart.
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[Journal Article] promotes cardiac dysfunction in diabetic mellitus due to excessive mitochondrial respiration-mediated ROS generation and lipid accumulation Circ- Heart Fail2012
Author(s)
Nakamura H, Matoba S, Iwai-Kanai E, Kimata M, Hoshino A, Nakaoka M, Katamura M, Okawa Y, Mita Y, Ikeda K, Okigaki M, Adachi S, Tanaka H, Takamatsu T, Matsubara H
Volume
5巻
Pages
106-115
DOI
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