2011 Fiscal Year Final Research Report
Analyses of biological significance of GPI-anchored protein CD109 in mouse development
| Project/Area Number |
21590435
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Nagoya University |
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Principal Investigator |
MURAKUMO Yoshiki 名古屋大学, 大学院・医学系研究科, 准教授 (40324438)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 疾患モデル動物 |
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| Research Abstract |
We generated CD109 knockout mouse to assess biological significance of GPI-anchored protein CD109 in mouse development. In wild-type mouse, CD109 expression was detected in basal and suprabasal region of the epidermis and seminiferous tubules of the testis. CD109./. mice could be alive and showed transient hair-loss from postnatal day 7(P7) to P28. CD109./. male and female mice were fertile. Histopathological analyses revealed that epidermal hyperplasia was detected in CD109./. mice from P7 to P70, and ectatic hair follicles were apparent from p14 to p21. Since epidermal hyperplasia was seen in dorsal skin epidermis, which does not have hair and hair follicles, we thought that CD109-deficiency develops epidermal hyperplasia, which leads to ectatic hair follicle and hair-loss. Immunohistochemical analyses revealed that STAT3 phosphorylation was apparently upregulated compaired with wild-type mice, suggesting that STAT3 signaling may be associated with epidermal hyperplasia of CD109./. mice. Next, we tried to detect secreted CD109 and to identify its interaction partners in the serum of CD109 transgenic mice. Secreted CD109 in mouse serum was detected by Western blotting, however, we could not identify candidates of CD109-interaction proteins in this experiment. Then, we attempted to identify CD109-interaction proteins in the medium of CD109-overexpressing culture cells. We successfully identified a candidate of CD109-interaction protein in this experiment, and now we are investigating biological significance of the interaction between CD109 and the interaction protein.
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[Journal Article] Girdin is an intrinsic regulator of neuroblast chain migration in the rostral migratory stream of the postnatal brain2011
Author(s)
Wang Y, Kaneko N, Asai N, Enomoto A, Isotani-Sakakibara M, Kato T, Asai M, Murakumo Y, Ota H, Hikita T, Namba T, Kuroda K, Kaibuchi K, Ming G, Song H, Sawamoto K, Takahashi M.
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Journal Title
J Neurosci
Volume: 31
Pages: 8109-22
Peer Reviewed
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[Journal Article] Protective role of Gipie, a Girdin family protein, in endoplasmic reticulum stress responses in endothelial cells2011
Author(s)
Matsushita E, Asai N, Enomoto A, Kawamoto Y, Kato T, Mii S, Maeda K, Shibata R, Hattori S, Hagikura M, Takahashi K, Sokabe M, Murakumo Y, Murohara T, Takahashi M.
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Journal Title
Mol Biol Cell
Volume: 22
Pages: 736-47
Peer Reviewed
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[Journal Article] Loss of Sprouty2 partially rescues renal hypoplasia and stomach hypoganglionosis but not intestinal aganglionosis in Ret Y1062F mutant mice2011
Author(s)
Miyamoto R, Jijiwa M, Asai M, Kawai K, Ishida-Takagishi M, Mii S, Asai N, Enomoto A, Murakumo Y, Yoshimura A, Takahashi M.
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Journal Title
Dev Biol
Volume: 349
Pages: 160-8
Peer Reviewed
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[Journal Article] Correlation of pathological grade and tumor stage of urothelial carcinomas with CD 109 expression2010
Author(s)
Hagikura M, Murakumo Y, Hasegawa M, Jijiwa M, Hagiwara S, Mii S, Hagikura S, Matsukawa Y, Yoshino Y, Hattori R, Wakai K, Nakamura S, Gotoh M, Takahashi M.
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Journal Title
Pathol int
Volume: 60
Pages: 735-43
Peer Reviewed
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[Journal Article] Analysis of DOK-6 function in downstream signaling of RET in human neuroblastoma cells2010
Author(s)
Kurotsuchi A, Murakumo Y, Jijiwa M, Kurokawa K, Itoh Y, Kodama Y, Kato T, Enomoto A, Asai N, Terasaki H, Takahashi M.
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Journal Title
Cancer Sci
Volume: 101
Pages: 1147-55
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[Journal Article] Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus2009
Author(s)
Enomoto A, Asai N, Namba T, Wang Y, Kato T, Tanaka M, Tatsumi H, Taya S, Tsuboi D, Kuroda K, Kaneko N, Sawamoto K, Miyamoto R, Jijiwa M, Murakumo Y, Sokabe M, Seki T, Kaibuchi K, Takahashi M.
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Journal Title
Neuron
Volume: 63
Pages: 774-87
Peer Reviewed
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[Journal Article] Expression of Ret finger protein correlates with outcomes in endometrial cancer2009
Author(s)
Tsukamoto H, Kato T, Enomoto A, Nakamura N, Shimono Y, Jijiwa M, Asai N, Murakumo Y, Shibata K, Kikkawa F, Takahashi M.
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Journal Title
Cancer Sci
Volume: 100
Pages: 1895-901
Peer Reviewed
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