2011 Fiscal Year Final Research Report
Study of lung epithelial and lung cancer stem cells of the lung-specific gene-targeted mice ; analysis by lung epithelial sphere assay
| Project/Area Number |
21590440
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kumamoto University |
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Principal Investigator |
ITO Takaaki 熊本大学, 大学院・生命科学研究部, 教授 (70168392)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Tetuhiro 熊本大学, 大学院・生命科学研究部, 助教 (70359818)
UDAKA Naoko 熊本大学, 大学院・生命科学研究部, 助教 (90285106)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 遺伝子改変マウス / EGFR / Hes1 / 肺 / 幹細胞 / Sca1 / epithelial sphere |
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| Research Abstract |
Lung epithelial sphere(LES) formation assay seems a good experimental method monitoring lung epithelial stem cells, and sca1-positive cells contain many lung epithelial stem cells. LESs were formed from mouse fetal epithelial cells, which lack sca1-positive cells. The lung epithelial cells having human mutant EGFR gene have high proliferative activity. In these cells, occurrence of sca1-positive cells does no increase, but LEC formation was mildly increased. In addition, significance of stem-cell related molecules was studied using surgically resected samples and cell lines.
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Research Products
(8 results)
