Development of efficient bone marrow transplantation and its application for treatment of malignant tumor or regeneration therapy

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590447
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kansai Medical University
• Principal Investigator: ADACHI Yasushi 関西医科大学, 医学部, 非常勤講師 (10268336)
• Co-Investigator(Renkei-kenkyūsha): MINAMINO Keizo 関西医科大学, 医学部, 助教 (40509585)
• Project Period (FY): 2009 – 2011
• Keywords: 疾患モデル動物 / 骨髄内骨髄移植 / マグネット・ビーズ

Research Abstract

Intra-bone marrow-bone-marrow transplantation(IBM-BMT) is more efficientmethod for bone marrow transplantation than conventionalintra-venous bone marrow transplantation(BMT). In this study, we used magnet-beads and a magnet for IBM-BMT. First, we coated donor bone marrow cells(BMCs) with magnet-beads. Next, we transplantedthe donor BMCs into the recipient bone marrow of the tibia. A powerful magnet wascircumferentiallyset around the bone when the donor BMCs were injected into the bone. IBM-BMT with magnet-beads and a magnet showed rapid hematopoiesis of donor BMCs.

Research Products

• [Journal Article] 効率の良い骨髄移植法の開発とその応用-動物モデルを用いた研究の重要性- (2012)
  • Author(s): 足立靖、沖垣光彦、杉原昭、越路みのり、小柳津治樹、池袋一哉、冨田実、南野桂三、鈴木康弘、張玉明、金田浩由紀、岩崎真佳、小池保志、中野景司、向出裕美、重松明男、小池直子、石明、矢内誠司、今井雄二郎、嶋千絵子、下智比古、池原進
  • Journal Title: 公立豊岡病院紀要 Volume: No.23 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Dehydroxymethylepoxyquinomicin(DHMEQ) can suppress tumour necrosis factor-α production in lipopolysaccharide-injected mice, resulting in rescuing mice from death in vivo (2011)
  • Author(s): Shimo T, Adachi Y, Umezawa K, Okigaki M, Takaya J, Taniuchi S, Ikehara S, Kaneko K
  • Journal Title: Clin Exp Immunol Volume: vol.166, No.2 Pages: 299-306
  • Peer Reviewed
• [Journal Article] Early Inflammatory Reactions in Atherosclerosis Are Induced by Tyrosine Kinase Proline-Rich Tyrosine Kinase/Reactive Oxygen Species-Mediated Release of Tumor Necrosis Factor-{alpha} and Subsequent Activation of the p21Cip1/Ets-1/p300 System (2011)
  • Author(s): Katsume A, Okigaki M, Matsui A, Che J, Adachi Y, Kishita E, Yamaguchi S, Ikeda K, Ueyama T, Matoba S, Yamada H, Matsubara H
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: vol.31, No.5 Pages: 1084-92
  • Peer Reviewed
• [Journal Article] Combination of intra-bone marrow-bone marrow transplantation and subcutaneous donor splenocyte injection diminishes risk of GVHD and enhances survival rate (2011)
  • Author(s): Shi M, Adachi Y, Cui Y, Li M, Lian Z, Zhang Y, Yanai S, Shima C, Imai Y, Ikehara S
  • Journal Title: Stem Cells Dev Volume: vol.20, No.5 Pages: 759-68
  • Peer Reviewed
• [Journal Article] Endothelial FGF receptor signaling accelerates atherosclerosis (2011)
  • Author(s): Che J, Okigaki M, Takahashi T, Katsume A, Adachi Y, Yamaguchi S, Matsunaga S, Takeda M, Matsui A, Kishita E, Ikeda K, Yamada H, Matsubara H
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: Vol.300, No.1 Pages: H154-61
  • Peer Reviewed
• [Journal Article] Klotho is associated with VEGF receptor-2 and the transient receptor potential canonical-1 Ca2+channel to maintain endothelial integrity (2011)
  • Author(s): Kusaba T, Okigaki M, Matui A, Murakami M, Ishikawa K, Kimura T, Sonomura K, Adachi Y, Shibuya M, Shirayama T, Tanda S, Hatta T, Sasaki S, Mori Y, Matsubara H
  • Journal Title: Proc Natl Acad Sci USA Volume: Vol.107, No.45 Pages: 19308-13
  • Peer Reviewed
• [Journal Article] Signaling from Fibroblast Growth Factor Receptor 2 in Immature Hematopoietic Cells Facilitates Donor Hematopoiesis after Intra-Bone Marrow-Bone Marrow Transplantation (2010)
  • Author(s): Shigematsu A, Shi M, Okigaki M, Adachi Y, Koike N, Che J, Iwasaki M, Matsubara H, Imamura M, Ikehara S
  • Journal Title: Stem Cells Dev Volume: vol.19, No.11 Pages: 1679-86
  • Peer Reviewed
• [Journal Article] Bone marrow-derived stromal cells can express neuronal markers by DHA/GPR40 signaling (2010)
  • Author(s): Kaplamadzhiev DB, Hisha H, Adachi Y, Ikehara S, Tonchev AB, Boneva NB, Pyko IV, Kikuchi M, Nakaya M, Wakayama T, Iseki S, Yamashima T
  • Journal Title: Biosci Trends Volume: vol.4, No.3 Pages: 119-29
  • Peer Reviewed
• [Journal Article] Combination Method of Magnetic Beads and Magnet Helps Sustain Number of Donor Bone Marrow Cells after Intra-Bone Marrow Injection, Resulting in Rapid Hemopoietic Recovery (2010)
  • Author(s): Shima C, Adachi Y, Shi M, Imai Y, Okigaki M, Yanai S, Takahashi K, Ikehara S
  • Journal Title: Bone Marrow Transplant Volume: vol.45, No.6 Pages: 993-9
• [Journal Article] Adult bone marrow cells can differentiate into hemopoietic cells and endothelial cells but not into other lineage cells in normal growth and normal life (2010)
  • Author(s): Yanai S, Adachi Y, Shi M, Shigematsu A, Shima C, Imai Y, Kwon AH, Ikehara S
  • Journal Title: Int J Hematol Volume: vol.91 Pages: 213-8
  • Peer Reviewed
• [Journal Article] Caspase Inhibitor, ZVAD-fmk, FacilitatesEngraftment of Donor Hematopoietic Stem Cells in Intra-Bone Marrow-Bone Marrow Transplantation (2010)
  • Author(s): Imai Y, Adachi Y, Shi M, Shima C, Yanai S, Okigaki M, Yamashima T, Kaneko K, Ikehara S
  • Journal Title: Stem Cells Dev Volume: vol.19 Pages: 461-8
  • Peer Reviewed
• [Journal Article] Intra-bone marrow transplantation slows disease progression and prolongs survival in G93A mutant SOD1 transgenic mice, a model mouse for amyotrophic lateral sclerosis (2009)
  • Author(s): Ohnishi S, Ito H, Suzuki Y, Adachi Y, Wate R, Zhang J, Nakano S, Kusaka H, Ikehara S
  • Journal Title: Brain Res Volume: vol.1296 Pages: 216-24
  • Peer Reviewed
• [Journal Article] Abnormal distribution of dendritic cells in(NZWxBXSB) F1mice (2009)
  • Author(s): Tokuyama Y, Adachi Y, Minamino K, Shintaku H, Okigaki M, Hayashi K, Kitajima A, Takaki T, Koike N, Shima C, Imai Y, Shi M, Yanai S, Ikehara S
  • Journal Title: Autoimmunity Volume: vol.42 Pages: 399-405
  • Peer Reviewed
• [Journal Article] Anti-tumor effectsof fusion Cells oftype 1 dendritic cells and tumor cells(Meth A) using Hemagglutinating Virus of Japan-Envelope(HVJ-E) (2009)
  • Author(s): Yanai S, AdachiY, Fujisawa JI, JiangSW, Okigaki M, Shi M, Shima C, Imai Y, Kwon AH, Ikhara S
  • Journal Title: Int J Oncol Volume: vol.35 Pages: 249-255
  • Peer Reviewed
• [Journal Article] Endothelium-targeted overexpression of constitutively active FGF receptor induces cardioprotection in mice myocardial infarction (2009)
  • Author(s): Matsunaga S, Okigaki M, Takeda M, Matsui A, Honsho S, Katsume A, Kishita E, Jishan C, Kurihara T, Adachi Y, Mansukhani A, Kobara M, MatobaY, Tatsumi T, Matsubara H
  • Journal Title: J Mol Cell Cardiol Volume: vol.46, No.5 Pages: 663-73
  • Peer Reviewed
• [Presentation] より効率的な骨髄内骨髄移植法の開発-マグネット・ビーズを用いて- (2012)
  • Author(s): 足立靖、嶋千絵子、南野桂三、石明、高橋寛二、池原進
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 20120612-14
• [Presentation] マグネット・ビーズを用いた骨髄内骨髄移植法 (2012)
  • Author(s): 石明、嶋千絵子、足立靖、南野桂三、高橋寛二、池原進
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京(京王プラザホテル)
  • Year and Date: 20120426-28
• [Presentation] LPS投与マウスにおけるDHMEQ(NF-κB inhibitor)の効果 (2012)
  • Author(s): 足立靖、下智比古、梅澤一夫、金子一成、池原進
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京(京王プラザホテル)
  • Year and Date: 20120426-28
• [Presentation] Heparin mobilizes multipotent human mesoangioblasts (2011)
  • Author(s): Fujitaka K, Iwasaki M, Sato D, Park H, Enoki C, Koyanagi M, Minato N, Otani H, Nishikawa M, Adachi Y, Ikehara S, Zeiher AM, Dimmeler S, Iwasaka T
  • Organizer: American Heart Association(AHA) scientific sessions 2011
  • Place of Presentation: Orland, Florida, USA(Orange Country Convention Center)
  • Year and Date: 20111112-16
• [Presentation] エンドトキシンショックにおけるNF-κB阻害剤の有用性:マウスを用いた検討 (2011)
  • Author(s): 下智比古、高屋淳二、谷内昇一郎、足立靖、金子一成
  • Organizer: 第114回日本小児科学会総会・学術集会
  • Place of Presentation: 東京(グランドプリンスホテル新高輪国際館パミール、グランドプリンスホテル高輪)
  • Year and Date: 20110812-14
• [Presentation] Dehydroxymethylepoxyquinomicin(DHMEQ), a novel inhibitor for NF-κB, ameliorates proteinuria in murine puromycin-aminonucleoside-induced nephrosis (2011)
  • Author(s): Shimo T, Adachi Y, Kimata T, Umezawa K, Ikehara S, Kaneko K
  • Organizer: The 11th AsianCongress of Pediatric Nephrology
  • Place of Presentation: Fukuoka, Japan(福岡国際会議場)
  • Year and Date: 20110602-04
• [Presentation] 網膜虚血再灌流モデルに対するG-CSFの効果 (2011)
  • Author(s): 嶋千絵子、足立靖、南野桂三、池原進、高橋寛二
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京東京国際フォーラム
  • Year and Date: 20110512-15
• [Presentation] アポトーシス阻害薬Z-VAD-fmkを用いた効率のよい骨髄内骨髄移植法 (2011)
  • Author(s): 足立靖、今井雄一郎、石明、池原進
  • Organizer: 第100回日本病理学会学術集会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 20110428-30
• [Presentation] 骨髄内骨髄移植と皮下donor lymphocyte infusionの組み合わせ効果 (2010)
  • Author(s): 石明、足立靖、池原進
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京京王プラザ
  • Year and Date: 20100427-29
• [Presentation] 胸腺移植を併用した骨髄内骨髄移植の悪性腫瘍に対する効果:胎生、新生仔、成体期の胸腺と造血幹細胞の比較 (2010)
  • Author(s): 保坂直樹、高木孝士、足立靖、池原進
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京京王プラザ
  • Year and Date: 20100427-29
• [Presentation] 特別シンポジウムより効率的な骨髄内骨髄移植の検討-コラーゲン・ゲル或いはマグネット・ビーズを用いて (2009)
  • Author(s): 足立靖、石明、池原進
  • Organizer: 第45回日本移植学会
  • Place of Presentation: 東京
  • Year and Date: 20090916-18
• [Presentation] 骨髄由来樹状細胞の骨内投与による抗腫瘍効果の検討 (2009)
  • Author(s): 嶋千絵子、足立靖、石明、矢内勢司、今井雄一郎、高橋寛二、池原進
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都(国際会議場)
  • Year and Date: 20090501-03
• [Presentation] 樹状細胞とマウス線維肉腫との融合細胞を用いた抗腫瘍効果の検討 (2009)
  • Author(s): 矢内勢司、足立靖、石明、嶋千絵子、今井雄一郎、池原進
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都(京都国際会議場)
  • Year and Date: 20090501-03
• [Presentation] 骨髄内骨髄移植法におけるコラーゲン・ゲルの有用性 (2009)
  • Author(s): 石明、足立靖、矢内勢司、嶋千絵子、今井雄一郎、池原進
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都(京都国際会議場)
  • Year and Date: 20090501-03
• [Presentation] Tie2発現細胞特異的にFGFレセプター2を発現させたトランスジェニックマウスにおける造血系細胞の解析 (2009)
  • Author(s): 足立靖、石明、矢内勢司、嶋千絵子、今井雄一郎、池原進
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都(京都国際会議場)
  • Year and Date: 2009-05-01