2011 Fiscal Year Final Research Report
The significance of the biosynthesis of plant hormones in Toxoplasma gondii
| Project/Area Number |
21590460
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | トキソプラズマ / 植物ホルモン |
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| Research Abstract |
Recently, we found that Toxoplasma gondii produces a plant hormone, abscisic acid, and that it is essential for the infection of this parasite. So, we studied the effect of other plant hormones cytokinins on Toxoplasma gondii. We found that genes encoding the enzymes for the trans-zeatin biosynthesis in plants are present in T. gondii genome. Consistently, we detected intermediates for two possible trans-zeatin-biosynthesis pathways by MS analysis. A natural cytokinin, trans-zeatin, accelerated the proliferation of T. gondii. In contrast, a synthetic cytokinin, thidiazuron, inhibited the parasite proliferation in culture. This inhibition was also observed in laboratory animals. Since cytokinins regulate the cell cycle progression by enhancing the expression of D-type cyclin in plant, we carried out flowcytometric analysis to observe the effect of cytokinins on the cell cycle progression of T. gondii. Trans-zeatin hastened the cell cycle progression from G1 to S phase while thidiazuron caused the halt there. Quantitative-PCR(qPCR) analysis revealed that trans-zeatin upregulated the expression of one cyclin like as higher plant system, whereas thidiazuron downregulated its expression. These results suggest that this cyclin plays a crucial role in controlling the cell cycle progression in T. gondii and concomitantly its proliferation. Since cytokinins are also known to regulate chloroplast development in plants, we examined whether cytokinins affect apicoplast, which is a homologous organella to chloroplast in T. gondii. Immunofluorescence microscopy and qPCR analysis revealed trans-zeatin increased the number of apicoplasts but thidiazuron extinguished apicoplast. These result suggest that cytokinins are very important molecules to regulate the cell cycle of the parasite and division of apicoplast.
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Research Products
(54 results)
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[Journal Article] Gibberellin Biosynthetic Inhibitors Make Human Malaria Parasite Plasmodium falciparum Cells Swell and Rupture to Death2012
Author(s)
Toyama, T., Tahara, M., Nagamune, K., Arimitsu, K., Hamashima, Y., Palacpac, N. M. Q., Kawaide, H., Horii, T., and Tanabe, K.
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Journal Title
PLoS ONE
Volume: 7(3)
Pages: e32246
Peer Reviewed
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[Journal Article] ATF6βis a host cellular target of the Toxoplasma virulence factor ROP182011
Author(s)
Yamamoto, M., Ma, J. S., Mueller, C., Kamiyama, N., Saiga, H., Kubo, E., Kimura, T., Okamoto, T., Megumi, Okuyama, M., Kayama, H., Nagamune, K., Takashima, S., Matsuura, Y., Soldati-Favre, D., and Takeda, K.
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Journal Title
J. Exp. Med
Volume: 208
Pages: 1533-46
Peer Reviewed
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[Presentation] Analyze of the mechanism of action of primaquine on Toxoplasma gondii2011
Author(s)
Fkshi, M., Aonuma, H., Tahara, M., Andrabi, S. B. A., and Nagamune, K.
Organizer
11th International Congress on Toxoplasmosis
Place of Presentation
Ottawa, Canada
Year and Date
20110600
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[Presentation] Plant hormone cytokinins : Elucidating their role in Toxoplasma gondii2011
Author(s)
Andrabi, S. B. A., Tahara, M., Aonuma, H., Toyama, T., Tanabe, K., Nozaki, T., and Nagamune, K.
Organizer
11th International Congress on Toxoplasmosis
Place of Presentation
Ottawa, Canada
Year and Date
20110600
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[Presentation] Plant hormone cytokinins : Elucidating their role in Toxoplasma gondii2011
Author(s)
Andrabi, S. B. A., Tahara, M., Aonuma, H., Toyama, T., Tanabe, K., Nozaki, T., and Nagamune, K.
Organizer
45th Annual Japan-U. S. Joint Conference on Parasitic Diseases
Place of Presentation
Tokyo
Year and Date
20110100
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[Presentation] Plant hormone cytokinins : Elucidating their role in Toxoplasma gondii2010
Author(s)
Andrabi, S. B. A., Tahara, M., Aonuma, H., Toyama, T., Tanabe, K., Nozaki, T., Nagamune, K
Organizer
Molecular Parasitology Meeting XXI
Place of Presentation
Woods Hole, MA, USA
Year and Date
20100900
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[Presentation] The effect of plant hormone cytokinins on Toxoplasma gondii2010
Author(s)
Andrabi, S. B. A., Tahara, M., Aonuma, H., Toyama, T., Tanabe, K., Nozaki, T., Nagamune, K
Organizer
The 18th Meeting of the International Society for Evolutionary Protistology
Place of Presentation
Kanazawa, Japan
Year and Date
20100700
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[Presentation] The effect of plant hormone cytokinins on Toxoplasma gondii2010
Author(s)
Andrabi, S. B. A., Tahara, M., Aonuma, H., Toyama, T., Tanabe, K., Nozaki, T., Nagamune, K
Organizer
第79回日本寄生虫学会
Place of Presentation
旭川
Year and Date
20100500
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