Molecular mechanism of tumorigenesis of cholangiocarcinoma induced by Opisthorchis viverrini infection

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Gifu University
• Principal Investigator: WU Zhiliang 岐阜大学, 医学系研究科, 助教 (90313874)
• Co-Investigator(Kenkyū-buntansha): NAGANO Isao 岐阜大学, 大学院・医学系研究科, 准教授 (40283296)
• Research Collaborator: THIDRUT Boonmars タイコンケン大学, 医学部, 准教授
• Project Period (FY): 2009 – 2011
• Keywords: タイ肝吸虫 / 胆管癌 / 発癌 / 分子機構 / マイクロアレイ

Research Abstract

In the present study, by using animal model of opisthorchiasis-associated cholangiocarcinoma(CCA) and the analysis of CCA tumor tissues of the patients from epidemic areas in Thailand, the genes and signaling pathways to involve in the tumorigenesis, and the biomarkers correlated with stage, metastasis and survival were identified. The study revealed the molecular mechanism that chronic inflammation caused by Opisthorchis viverrini infection decreases the ability of detoxination, leading to the accumulation of ROS and the toxin products from worm, which causes DNA damage, epigenesis and methylation, and expression change of the genes that promotes cell proliferation, differentiation, transformation and carcinogenesis.

Research Products

• [Journal Article] Alteration of galectin-1 during tumorigenesis of Opisthorchis viverrini infection-induced cholangiocarcinoma and its correlation with clinicopathology (2012)
  • Author(s): Wu Z, Boonmars T, Nagano I, Boonjaraspinyo S, Pinlaor S, Pairojkul C, Chamgramol Y, Takahashi Y
  • Journal Title: Tumour Biol
• [Journal Article] Anti-inflammatory, antioxidant and hepatoprotective effects of Thunbergia laurifolia Linn. on experimental opisthorchiasis (2012)
  • Author(s): Wonkchalee O, Boonmars T, Aromdee C, Laummaunwai P, Khunkitti W, Vaeteewoottacharn K, Sriraj P, Aukkanimart R, Loilome W, Chamgramol Y, Pairojkul C, Wu Z, Juasook A, Sudsarn P
  • Journal Title: Parasitol Res
• [Journal Article] Overexpression of PDGFA and its receptor during carcinogenesis of Opisthorchis viverrini-associated cholangiocarcinoma (2012)
  • Author(s): Boonjaraspinyo S, Wu Z, Boonmars T, Kaewkes S, Loilome W, Sithithaworn P, Nagano I, Takahashi Y, Yongvanit P, Bhudhisawasdi V
  • Journal Title: Parasitol Int Volume: 61 Pages: 145-50
• [Journal Article] Comparative studies on animal models for Opisthorchis viverrini infection : host interaction through susceptibility and pathology (2012)
  • Author(s): Wonkchalee O, Boonmars T, Kaewkes S, Chamgramol Y, Aromdee C, Wu Z, Juasook A, Sudsarn P, Boonjaraspinyo S, Pairojkul C
  • Journal Title: Parasitol Res Volume: 110 Pages: 1213-23
• [Journal Article] Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis (2011)
  • Author(s): Juasook A, Boonmars T, Kaewkes S, Loilome W, Veteewuthacharn K, Wu Z, Yongvanit P
  • Journal Title: Parasitol Res
• [Journal Article] Candidate genes involving in tumorigenesis of cholangiocarcinoma induced by Opisthorchis viverrini infection (2011)
  • Author(s): Wu Z, Boonmars T, Boonjaraspinyo S, Nagano I, Pinlaor S, Puapairoj A, Yongvanit P, Takahashi Y
  • Journal Title: Parasitol Res Volume: 109 Pages: 657-73
• [Journal Article] Differential detection of Trichinella papuae, T. spiralis and T. pseudospiralis by real-time fluorescence resonance energy transfer PCR and melting curve analysis (2011)
  • Author(s): Tantrawatpan C, Intapan PM, Thanchomnang T, Lulitanond V, Boonmars T, Wu Z, Morakote N, Maleewong W
  • Journal Title: Vet Parasitol Volume: 185 Pages: 210-5
• [Journal Article] Indirect effect of a turmeric diet : enhanced bile duct proliferation in Syrian hamsters with a combination of partial obstruction by Opisthorchis viverrini infection and inflammation by N-nitrosodimethylamine administration (2011)
  • Author(s): Boonjaraspinyo S, Boonmars T, Aromdee C, Puapairoj A, Wu Z
  • Journal Title: Parasitol Res Volume: 108 Pages: 7-14
• [Journal Article] Opisthorchis viverrini infection causes liver and biliary cirrhosis in gerbils (2011)
  • Author(s): Wonkchalee O, Boonmars T, Kaewkes S, Chamgramol Y, Pairojkul C, Wu Z, Juasook A, Sudsarn P, Boonjaraspinyo S
  • Journal Title: Parasitol Res Volume: 109 Pages: 545-51
• [Journal Article] Involvement of c-Ski Oncoprotein in Carcinogenesis of Cholangiocacinoma Induced by Opisthorchis viverrini and N-nitrosodimethylamine (2011)
  • Author(s): Boonmars T, Wu Z, Boonjaruspinyo S, Puapairoj A, Kaewsamut B, Nagano I, Pinlaor S, Yongvanit P, Wonkchalee O, Juasook A, Sudsarn P, Srisawangwong T
  • Journal Title: Pathol Oncol Res Volume: 17 Pages: 219-27
• [Journal Article] Effect of curcumin on pathogenesis of hamster-opisthorchiasis through apoptosis-related gene expression (2009)
  • Author(s): Sriraj P, Boonmars T, Boonjaraspinyo S, Kaewsamut B, Srisawangwong T, Sithithaworn P, Wu Z
  • Journal Title: Southeast Asian J Trop Med Public Health Volume: 40 Pages: 1208-15
• [Journal Article] Alterations of gene expression of RB pathway in Opisthorchis viverrini infection-induced cholangiocarcinoma (2009)
  • Author(s): Boonmars T, Wu Z, Boonjaruspinyo S, Pinlaor S, Nagano I, Takahashi Y, Kaewsamut B, Yongvanit P
  • Journal Title: Parasitol Res Volume: 105 Pages: 1273-81
• [Presentation] Involvement of platelet-derived growth factor alpha in tumorigenesis of cholangiocarcinoma induced by Opisthorchis viverrini infection (2011)
  • Author(s): Sirintip Boonjaraspinyo, Zhiliang Wu, Thidarut Boonmars, Isao Nagano, Sasithorn Kaewkes, Watchalin Loilome, Puangrat Yongvanit, Vajarabhongsa Bhudhisawasdi, Yuzo Takahashi
  • Organizer: 第80回日本寄生虫学会大会
  • Year and Date: 2011-07-18
• [Presentation] タイ肝吸虫(Opisthorchis viverrini)感染による胆管癌の発癌の分子機序の研究 (2011)
  • Author(s): 呉志良、Sirintip Boonjaraspinyo、Thidarut Boonmars、長野功、Anucha Puapairoj、Butsara Kaewsamut、Puangrat Yongvani、浅野一信、高橋優三
  • Organizer: 第80回日本寄生虫学会大会
  • Year and Date: 2011-07-18
• [Presentation] Expression of Platelet-derived growth factor alpha in Cholangiocarcinoma and its Clinicopathological Significance (2011)
  • Author(s): Boonmars T, Boonjaraspinyo S, Wu Z, Nagano I, Kaewkes S, Takahashi Y, Loilome W, Yongvanit P, Bhudhisawasdi V
  • Organizer: The Latest Advances in Liver Cancer Research
  • Year and Date: 2011-03-01
• [Presentation] タイ肝吸虫の感染による胆管癌におけるgalectin遺伝子の発現 (2010)
  • Author(s): 呉志良、Sirintip Boonjaraspinyo、Thidarut Boonmars、長野功、浅野一信、高橋優三
  • Organizer: 第66回日本寄生虫学会西日本支部大会
  • Year and Date: 2010-11-06
• [Presentation] RBシグナル経路はタイ肝吸虫感染による胆管癌の発癌に関与する (2009)
  • Author(s): 呉志良、Thidurat Boonmars、長野功、浅野一信、Sirintip Boonjaruspinyo、Somchai Pinlaor、Butsara Kaewsamut、Puangrat Yongvanit、高橋優三
  • Organizer: 第65回日本寄生虫学会西日本支部大会
  • Year and Date: 2009-11-08