2011 Fiscal Year Final Research Report
Studies on emesis-inducing signal receptor and molecular transfer mechanism of staphylococcal enterotoxin
| Project/Area Number |
21590475
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
HU Dong-liang 弘前大学, 大学院・医学研究科, 准教授 (10333733)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANE Akio 弘前大学, 大学院・医学研究科, 教授 (30164239)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 病原性 / エンテロトキシ |
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| Research Abstract |
Staphylococcal enterotoxins (SEs) are superantigenic toxins and involved in human diseases including food poisoning and toxic shock syndrome. However, the role and mechanism in clinical symptoms of food poisoning remains poorly understood. In this study, house musk shrews were used to study the role and mechanism of SEs in food poisoning. Oral administration of SEA into shrews elicited a strong emetic response but no diarrhoeagenic symptom. SEA was injected into the intestinal loops of the animals did not induce exudation of fluid and consequent dilation of the intestinal segments. These results indicate that SEA has a strong superantigenic and emetic activity, but no diarrhoeagenic activity. We further investigated SEA receptor and emetic signal pathway in house musk shrews. Our results showed that pretreatment with a vanilloid receptor antagonist, capsazepine, significantly inhibited SEA-induced vomiting responses in the animals and the effect was shown in a dose-dependent manner. Immunofluorescence analysis showed that intestine of house musk shrew express vanilloid receptor and SEA co-localized with the cells expressing vanilloid receper. These results suggest that vanilloid receptor could play an important role in SEA-induced emetic response and this finding might be useful in prevention and treatment of staphylococcal food poisoning.
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[Journal Article] Submucosal mast cells in the gastrointestinal tract are a target of staphylococcal enterotoxin type A2012
Author(s)
Ono, H., Nishizawa, M., Yamamoto, Y., Hu, D.-L., Nakane, A Shinagawa, K. and Omoe, K.
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Journal Title
FEMS Immun. Med. Microbiol
Volume: 64(1)
Pages: 392-402
Peer Reviewed
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[Journal Article] The superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzymes2011
Author(s)
Li, S.-J., Hu, D.-L., Maina, E. K., Shinagawa, K., Omoe, K. and Nakane, A
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Journal Title
J. Appl. Microbiol
Volume: 110
Pages: 729-736
Peer Reviewed
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[Journal Article] High affinity of interaction between superantigen and TCR Vb molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells2010
Author(s)
Omoe, K., Nunomora, W., Kato, H., Li, Z.-J., Igarashi, O., Araaki, M., Sano, K., Ono, H., Abe, Y., Hu, D.-L., Nakane, A., Kiyono, H., Takakuwa, Y., Shinagawa, K., Uchiyama, T., Imanishi, K.
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Journal Title
J. Bio. Chem
Volume: 285(40)
Pages: 30427-30435
Peer Reviewed
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[Journal Article] Role of interleukin-17A in cell-dediated protection against Staphylococcus aureus infection in mice immunized with fibrinogen-binding domain of clumping factor A2010
Author(s)
Narita, K., Hu, D.-L., Mori, F., Wkabayashi, K., Iwakura, Y. and Nakane, A
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Journal Title
Infect. Immun
Volume: 78(10)
Pages: 4999-5005
Peer Reviewed
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[Journal Article] Protective effect of glutathione S-transferase-fused mutant staphylococcal entero-toxin C against Staphylococcus aureus-induced bovine mastitis2010
Author(s)
Cui, J.-C., Zhang, B.-J., Lin, Y.-C., Wang, Q.-K., Qian, A.-D., Nakane, A., Hu, D.-L. and Tong, G.-Z
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Journal Title
Vet. Immun. Immunopath
Volume: 135(5)
Pages: 64-70
Peer Reviewed
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[Journal Article] Immuniza-tion with glutathione S-transferase-fused mutant staphylococcal entero toxin C protects against Staphylo-coccus aureus-induced mastitis in mouse model2009
Author(s)
Zhang, B.-J., Cui, J.-C., Zhang, X., Qian, A.-D., Tong, G.-Z., Hu, D.-L.-, Nakane, A. and Wang Q.-K
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Journal Title
Chn. J. Prev. Vet. Med. (Chinese Journal of Preventive Veterinary Medicine)
Volume: 31(7)
Pages: 557-562
Peer Reviewed
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[Journal Article] evelopment and evaluation of Staphylococcus aureus-induced mastitis model2009
Author(s)
Zhang, B.-J., Cui, J.-C., Zhang, X., Qian, A.-D., Tong, G.-Z. , Hu, D.-L., Nakane, A. and Wang Q.-K
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Journal Title
Chn. J. Prev. Vet. Med. (Chinese Journal of Preventive Veterinary Medicine)
Volume: 31(5)
Pages: 365-369
Peer Reviewed
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[Journal Article] c-di-GMP as a vaccine adjuvant protects against systemic methici-llin-resistant Staphylococcus aureus (MRSA) infection2009
Author(s)
Hu, D.-L., Narita, K., Hyodo, M., Hayakawa, Y., Nakane, A. and Karao l i s, D. K
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Journal Title
Vaccine
Volume: 27
Pages: 4867-4873
Peer Reviewed
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[Presentation] Superantigenic activity of toxic shock syndrome toxin-1 is relatively resistant to heating and digestive enzymes compared with staphylococcal entetotoxin A2011
Author(s)
Hu, D.-L, Li, S.-J., Maina, E.K., Omoe, K., Nakane, A
Organizer
IUMS, Sapporo
Place of Presentation
Japan.
Year and Date
20110906-10
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[Presentation] Omoe, K. temperiture dependent regulation of enterotoxin gene cluster related staphylococcal entero toxins production2010
Author(s)
Sato, A., Nagasako, Y., Yamamoto, Y., Sato, Y., Ono, H. K., Hu, D.-L., Nakane, A
Organizer
IUMS, Sapporo
Place of Presentation
Japan
Year and Date
20100906-11
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[Presentation] The superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzyme treatment2010
Author(s)
Hu, D.-L. , Maina, E. K., Tomioka, Y., Li, S.-J., Narita, K., Osanai, A., Shinagawa, K., Omoe, K. and Nakane, A
Organizer
14^<th> International Symposium on Staphy-lococci and Staphylococcal Infections
Place of Presentation
Bath, United Kingdom
Year and Date
20100906-09
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[Presentation] Vaccination with a nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews2010
Author(s)
Hu, D.-L., Omoe, K., Sashinami, H., Shinagawa, K., Nakane, A
Organizer
The 10th Japan-Korea International Symposium on Micro-biology
Place of Presentation
Yokohama, Japan
Year and Date
2010-03-26
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[Presentation] Vaccination with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews2009
Author(s)
Hu, D.-L., Omoe, K., Sashinami, H., Shinagawa, K., Nakane, A.
Organizer
第39回日本免疫学会総会
Place of Presentation
大阪
Year and Date
20091202-04
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