2011 Fiscal Year Final Research Report
Genetic analysis of the Sec-dependent class IIa-type enterococcal bacteriocins.
| Project/Area Number |
21590476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 腸球菌 / 抗菌物質 / バクテリオシン / 腸管内定着 / 日和見感染菌 |
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| Research Abstract |
Genetic analysis of Bac31 and Bac43, which are the Sec-dependent class IIa-type enterococcal bacteriocins, were performed. The chimera mutants composed of two bacteriocins were constructed and analyzed. Two amino acid residues positioned at 13th and 24th were significance for the activities. The factors concerning the expression and secretion of bacteriocin were as follows, gene copy number, promoter activity, signal peptide, and host bacteria. Of the factors, the signal peptide was the most crucial for the secretion level of bacteriocin. The immunity (resistance) genes for Bac31 and Bac43 were confirmed using a newly-constructed gene-expression controllable plasmid vector named pHT7343A.
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Research Products
(16 results)
