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2011 Fiscal Year Final Research Report

Functional characterization of type III secretion apparatus STM1410 encoded by Salmonella pathogenicity island 2

Research Project

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Project/Area Number 21590490
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKitasato University

Principal Investigator

OKADA Nobuhiko  北里大学, 薬学部, 教授 (80194364)

Co-Investigator(Kenkyū-buntansha) MIKI Tsuyoshi  北里大学, 薬学部, 助教 (40398582)
Project Period (FY) 2009 – 2011
Keywords病原性 / サルモネラ感染症 / III型分泌機構
Research Abstract

The virulence of many Gram-negative pathogens is associated with type III secretion systems(T3SSs), which deliver virulence effector proteins into the cytoplasm of host cells. Components of Salmonella T3SS are encoded within Salmonella pathogenicity island I and II(SPI-1 and SPI-2). While most SPI-2 T3SS proteins in Salmonella have assigned names and functions, a few of them remain poorly characterized. Here, we studied a SPI-2-encoded protein, STM1410, that shows no homology to other T3SS/flagellar proteins and is only present in pathogenic Salmonella. Our findings demonstrated that it is essential for type III secretion and that it is localized to the inner membrane. Interestingly, we found STM1410 is critical for the stability for the SPI-2 effector protein Pip B. Overall, our findings suggest that STM1410 is a structural protein that contributes to the function of the T3S complex, and therefore we propose to rename it Ssa W.

  • Research Products

    (28 results)

All 2012 2011 2010 2009

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (19 results)

  • [Journal Article] Salmonella type III effectoor SpvC, a phosphothreonine lyase, contributes to reduction in inflammatory response during intestinal phase of infection2012

    • Author(s)
      Haneda T, Y. Ishii, H. Shimizu, K. Ohshima, N. Iida, H. Danbara, and N. Okada
    • Journal Title

      Cell. Microbiol

      Volume: 14 Pages: 485-499

    • Peer Reviewed
  • [Journal Article] Construction and evaluation of Salmonella enterica slyA mutant strain for use in live attenuated oral vaccines2011

    • Author(s)
      Haneda T, N. Okada, Y. Kikuchi, M. Takagi, T. Kurotaki, T. Miki, S. Arai and H. Danbara
    • Journal Title

      Comp. Immunol. Microbiol. Infect. Dis

      Volume: 34 Pages: 399-409

    • Peer Reviewed
  • [Journal Article] The Chromobacterium type III effector CopE, a guanine nucleotide exchange factor for Rac1 And Cdc42, is involved in bacterial invasion of epithelial cells and pathogenesis2011

    • Author(s)
      Miki, T., K. Akiba, M. Iguchi, H. Danbara and N. Okada
    • Journal Title

      Mol. Microbiol

      Volume: 80 Pages: 1186-1203

    • Peer Reviewed
  • [Journal Article] Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages2010

    • Author(s)
      Haneda, H., M. Sugimoto, Y. Yoshida-Ohta, Y. Kodera, M. Oh-Ishi, T. Maeda, S. Shimizu-Izumi, T. Miki, Y. Kumagai, H. Danbara, and N. Okada
    • Journal Title

      BMC Mocrobiol

      Volume: 10 Pages: 324

    • DOI

      doi:10.1186/1471-2180-10-324

    • Peer Reviewed
  • [Journal Article] Chromobacterium pathogenicity island 1 type III secretion system is a major virulence determinant for Chromobacterium violaceum-induced cell death in hepatocytes2010

    • Author(s)
      Miki, T., M. Iguchi, K. Akiba, M. Hosono, T. Sobue, H. Danbara and N. Okada
    • Journal Title

      Mol. Microbiol

      Volume: 77 Pages: 855-872

    • Peer Reviewed
  • [Journal Article] Oral administration of heat-killed Lactobacillus plantarum strain b240 protects mice from Salmonella enterica serovar Typhimurium2010

    • Author(s)
      Ishikawa, H., E. Kutsukake, T. Fukui, I. Sato, T. Shirai, T. Kurihara, N. Okada, H. Danbara, M. Toba, N. Kohda, Y, Maeda, and T. Matsumoto
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 74 Pages: 1338-1342

    • Peer Reviewed
  • [Journal Article] A practical random mutagenesis system for probiotic Lactobacillus casei using Tn5 transposition complexes2010

    • Author(s)
      Ito, M., Y-G, Kim, H. Tsuji, M. Kiwaki, K. Nomoto, R. Tanaka, N. Okada, and H. Danbara
    • Journal Title

      J. Appl. Microbiol

      Volume: 109 Pages: 657-666

    • Peer Reviewed
  • [Journal Article] Functional characterization of SsaE, a novel chaperone protein of the type III secretion system encoded by Salmonella pathogenicity island 22009

    • Author(s)
      Miki, T., Y. Shibagaki, H. Danbara and N. Okada
    • Journal Title

      J. Bacteriol

      Volume: 191 Pages: 6843-6854

    • Peer Reviewed
  • [Journal Article] Genome-wide identification of novel genomic islands that contribute to Salmonella virulence in mouse systemic infection2009

    • Author(s)
      Haneda, T., Y. Ishii, H. Danbara and N. Okada
    • Journal Title

      FEMS Microbiol. Lett

      Volume: 298 Pages: 241-249

    • Peer Reviewed
  • [Presentation] Salmonella type 3 effector SpvC, a phosphothreonine lyase, contributes to reduction in inflammatory response during intestinal phase of infection.2011

    • Author(s)
      Haneda T, Okada N
    • Organizer
      第94回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      20111006-07
  • [Presentation] SpvC, a Salmonella effector reduses intestinal inflammation during early stage of infection2011

    • Author(s)
      Haneda T, Ishii Y, Shimizu H, Ohshima K, Danbara H, Okada N
    • Organizer
      FEMS 2011
    • Place of Presentation
      Geneva
    • Year and Date
      20110626-30
  • [Presentation] A Practical Random Mutagenesis System for Probiotic Lactobacillus casei Using Tn5 Transposition Complexes2011

    • Author(s)
      Ito M, Kim YG, Tsuji H, Kiwaki M, Nomoto K, Tanaka R, Danbara H, Okada N
    • Organizer
      FEMS 2011
    • Place of Presentation
      Geneva
    • Year and Date
      20110626-30
  • [Presentation] Lactobacillusにおけるランダム変異株作製法の確立2010

    • Author(s)
      伊藤雅洋、辻浩和、野本康二、岡田信彦、檀原宏文
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20100327-29
  • [Presentation] シグマ因子RpoNのリステリア病原性への関与とその機構2010

    • Author(s)
      岡田由美子、鈴木穂高、角田修子、五十君靜宏、山本茂貴、岡田信彦
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20100327-29
  • [Presentation] SpvCによるサルモネラ腸炎抑制機構の解析2010

    • Author(s)
      羽田健、石井雄太、岡田信彦、檀原宏文
    • Organizer
      第84回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20100327-29
  • [Presentation] Chromobacterium violaceumの病原性発現におけるIII型分泌機構の役割2010

    • Author(s)
      三木剛志、岡田信彦、檀原宏文
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20100327-29
  • [Presentation] 定量的RT-PCR法による高感度Chlamydia定量系の構築2010

    • Author(s)
      木田優美、倉川尚、高橋琢也、野本康二、岡田信彦、檀原宏文
    • Organizer
      第23回北里大学バイオサイエンスフォーラム
    • Place of Presentation
      青森
    • Year and Date
      2010-08-05
  • [Presentation] フィズス菌-大腸菌シャトルベクターを用いたヒト腸管由来ビフィズス菌の形質転換2010

    • Author(s)
      村井牧、伊藤雅洋、木脇真祐美、辻浩和、野本康二、岡田信彦、檀原宏文
    • Organizer
      第23回北里大学バイオサイエンスフォーラム
    • Place of Presentation
      青森
    • Year and Date
      2010-08-05
  • [Presentation] Lactobacillusにおけるランダム変異株作製法の確立2010

    • Author(s)
      伊藤雅洋、辻浩和、野本康二、岡田信彦、檀原宏文
    • Organizer
      第14回腸内細菌学会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-18
  • [Presentation] ビフィズス菌-大腸菌シャトルベクターを用いたヒト腸管由来ビフィズス菌の形質転換2010

    • Author(s)
      村井牧、伊藤雅洋、木脇真祐美、辻浩和、野本康二、岡田信彦、檀原宏文
    • Organizer
      第14回腸内細菌学会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-18
  • [Presentation] 定量的RT-PCR法による高感度Chlamydia定量系の構築2010

    • Author(s)
      木田優美、倉川尚、高橋琢也、野本康二、岡田信彦、檀原宏文
    • Organizer
      第84回日本感染症学会
    • Place of Presentation
      京都
    • Year and Date
      2010-04-05
  • [Presentation] サルモネラ感染と免疫応答2009

    • Author(s)
      羽田健
    • Organizer
      第92回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      20091105-06
  • [Presentation] サルモネラの緊縮応答と病原性2009

    • Author(s)
      岡田信彦
    • Organizer
      2009日本乳酸菌学会秋期シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2009-11-27
  • [Presentation] Salmonella pathogenicity island 2上にコードされるSsaNの機能解析2009

    • Author(s)
      小野紗矢佳、三木剛志、岡田信彦、檀原宏文
    • Organizer
      第92回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-05
  • [Presentation] サルモネラ腸炎モデルを用いたSalmonella plasmid virulence Cの機能解析2009

    • Author(s)
      石井雄太、羽田健、岡田信彦、檀原宏文
    • Organizer
      第92回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-05
  • [Presentation] Chromobacterium violaceumエフェクター候補因子CV2266の機能解析2009

    • Author(s)
      石井真利衣、三木剛志、秋庭きなり、岡田信彦、檀原宏文
    • Organizer
      第92回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-05
  • [Presentation] Lactobacillusにおける実用的なランダム変異株作製法の確立2009

    • Author(s)
      伊藤雅洋、岡田信彦、檀原宏文
    • Organizer
      第22回バイオサイエンスフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2009-08-03
  • [Presentation] プロテオーム解析によるサルモネラ緊縮応答タンパク質の同定2009

    • Author(s)
      岡田信彦
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13

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Published: 2013-07-31   Modified: 2014-12-15  

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