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2011 Fiscal Year Final Research Report

Elucidation of the pathological factor of Helicobacter pylori isolated from iron deficient anemia patient

Research Project

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Project/Area Number 21590492
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyorin University

Principal Investigator

OSAKI Takako  杏林大学, 医学部, 講師 (90255406)

Co-Investigator(Renkei-kenkyūsha) KAMIYA Shigeru  杏林大学, 医学部, 教授 (10177587)
HANAWA Tomoko  杏林大学, 医学部, 講師 (80255405)
KURATA Satoshi  杏林大学, 医学部, 助教 (00383670)
YONEZAWA Hideo  杏林大学, 医学部, 助教 (60453528)
Research Collaborator KATO Seiichi  杏林大学, 医学部, 非常勤講師
Project Period (FY) 2009 – 2011
KeywordsHelicobacter pylori / 鉄欠乏性貧血
Research Abstract

Many case reports and epidemiological studies suggest that Helicobacter pylori infection causes iron deficiency anemia(IDA), especially in children and adolescents. By DNA microarray analysis, we could compare expression of whole genes between IDA-related and control H. pylori strains. Seventeen genes with a significantly lower expression were shown. These include two genes, frpB1, frpB2 and ceuE, which are related to iron uptake and storage. We performed a microarray analysis comparing gene expression under iron-limited and iron-sufficient conditions. The expression of iron storage related gene, pfr, was lower under the limited condition for iron in all strains. IDA strains showed high expression of only frpB1, however control strains showed higher expression of fecA1, fecA2, frpB2.These results were also obtained from analysis of each gene expression by real time RT-PCR. The present study showed that different mechanisms between IDA strains and control strains for iron uptake system.
The expression of vacA gene, protein production of VacA in IDA strains was also higher than that in control strains. All IDA strains had cytotoxic effect on AGS cells, but only one control strain had. It is likely that the vacA gene plays some role in iron uptake system in H. pylori or host.

  • Research Products

    (47 results)

All 2012 2011 2010 2009

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (38 results)

  • [Journal Article] Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori2012

    • Author(s)
      Osaki T, Matsuki T, 他8名
    • Journal Title

      Microbial Pathogenesis

      Volume: 53 Pages: 12-18

    • Peer Reviewed
  • [Journal Article] The destructive effects of butyrate on the cell envelope of Helicobacter pylori2012

    • Author(s)
      Yonezawa H, Osaki T, 他9名
    • Journal Title

      J Med Microbiol

      Volume: 61 Pages: 582-589

    • Peer Reviewed
  • [Journal Article] Analysis of outer membrane vesicle protein involved in biofilm formation of Helicobacter pylori2011

    • Author(s)
      Yonezawa H, Osaki T, 他7名
    • Journal Title

      Anaerobe

      Volume: 17 Pages: 388-390

    • Peer Reviewed
  • [Journal Article] In vitro and in vivo effects of the Mongolian drug Amu-ru 7 on Helicobacter pylori growth and viability2010

    • Author(s)
      Bai CL, Osaki T, 他6名
    • Journal Title

      Microbiol Immunol

      Volume: 54 Pages: 508-515

    • Peer Reviewed
  • [Journal Article] Analysis of the microflora in the stomach of Mongolian gerbils infected with Helicobacter pylori2010

    • Author(s)
      Zaman C, Osaki T, 他4名
    • Journal Title

      J Gastroenterol Hepatol

      Volume: 25(Suppl. 1) Pages: S11-S14

    • Peer Reviewed
  • [Journal Article] In Vitro Antibacterial Activity of Phx-3 against Helicobacter pylori2010

    • Author(s)
      Hanawa T, Osaki T, 他5名
    • Journal Title

      Biol Pharm Bull

      Volume: 33 Pages: 188-191

    • Peer Reviewed
  • [Journal Article] Vertical Helicobacter pylori transmission from Mongolian gerbil mothers to pups2009

    • Author(s)
      Oshio I, Osaki T, 他5名
    • Journal Title

      J Med Microbiol

      Volume: 58 Pages: 656-662

    • Peer Reviewed
  • [Journal Article] Prevalence of Helicobacter and Acanthamoeba in natural environment2009

    • Author(s)
      Kawaguchi K, Matsuo J, Osaki T, Kamiya S, Yamaguchi H
    • Journal Title

      Lett Appl Microbiol

      Volume: 48 Pages: 465-471

    • Peer Reviewed
  • [Journal Article] Outer membrane vesicles of Helicobacter pylori TK1402 are involved in biofilm formation2009

    • Author(s)
      Yonezawa H, Osaki T, 他6名
    • Journal Title

      BMC Microbiol

      Volume: 9 Pages: 197

    • Peer Reviewed
  • [Presentation] Interaction between Helicobacter pylori and Caco-2 cells in the co-culture system2012

    • Author(s)
      大崎敬子、米澤英雄、, 他5名
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      20120327-29
  • [Presentation] The influence of biofilm formation of Helicobacter pylori on clarithromycin resistance2012

    • Author(s)
      米澤英雄、大崎敬子、, 他4名
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      20120327-29
  • [Presentation] Analysis of symbiotic conditions between Helicobacter pylori and protozoa2012

    • Author(s)
      北条史、大崎敬子、, 他4名
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      20120327-29
  • [Presentation] わが国小児のHelicobacter pylori感染源の検索2012

    • Author(s)
      菊地正悟、上田純子、柳生聖子、林櫻松、奥田真珠美、前川講平、福田能啓、大崎敬子、米澤英雄、神谷茂
    • Organizer
      第22回日本疫学会学術総会
    • Place of Presentation
      東京
    • Year and Date
      20120126-28
  • [Presentation] Prevalence o f Helicobacter pylori infection in children in a rural area of Japan2011

    • Author(s)
      Kikuchi S, Okuda M, Ueda J, Osaki T, 他6名
    • Organizer
      XXIV^<th> International Workshop of the Helicobacter Study Group
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      20110911-13
  • [Presentation] Intrafamilial transmission of Helicobacter pylori infection in a rural area of Japan2011

    • Author(s)
      Okuda M, Kikuchi S, Ueda J, Osaki T, 他6名
    • Organizer
      XXIV^<th> International Workshop of the Helicobacter Study Group
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      20110911-13
  • [Presentation] The characterization of Helicobacter pylori strains isolated from the iron deficiency anemia patients2011

    • Author(s)
      Osaki T, Kato S, 他6名
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20110906-16
  • [Presentation] In Vivo antibacterial activity of PHX-3 against Helicobacter pylori2011

    • Author(s)
      Hojo F, Osaki T, 他3名
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20110906-16
  • [Presentation] Oral bacteria affects the virulence of Helicobacter pylori2011

    • Author(s)
      Yonezawa H, Osaki T
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20110906-16
  • [Presentation] Analysis of iron transport and storage gene in Helicobacter pylori isolated from iron deficiency anemia patients2011

    • Author(s)
      Osaki T, Kato S, 他5名
    • Organizer
      The 16th International Workshop on Campylobacter, Helicobacter, and Related Organisms
    • Place of Presentation
      Vancouver
    • Year and Date
      20110828-0901
  • [Presentation] Analysis for microbial ecology between Helicobacter pylori and gastric microbiota in Mongolian gerbil2011

    • Author(s)
      Kamiya S, Zaman C, Yonezawa H, Hojo F, Osaki T
    • Organizer
      The 16th International Workshop on Campylobacter, Helicobacter, and Related Organisms
    • Place of Presentation
      Vancouver
    • Year and Date
      20110828-0901
  • [Presentation] Helicobacter pyloriとCaco-2細胞の混合培養時の相互作用について2011

    • Author(s)
      大崎敬子、加藤晴一, 他5名
    • Organizer
      第17回日本ヘリコバクター学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      20110624-25
  • [Presentation] In vivoにおけるPhx-3の抗Helicobacter pylori活性について2011

    • Author(s)
      北条史、大崎敬子, 他2名
    • Organizer
      第17回日本ヘリコバクター学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      20110624-25
  • [Presentation] Helicobacter pyloriのバイオフィルム形成とクラリスロマイシン耐性2011

    • Author(s)
      米澤英雄、大崎敬子, 他3名
    • Organizer
      第17回日本ヘリコバクター学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      20110624-25
  • [Presentation] 月見草エキスおよびフコイダン複合物の抗Helicobacter pylori作用2011

    • Author(s)
      大崎敬子, 岡田忠司, 李城杓, 神谷茂
    • Organizer
      第84回日本感染症学会総会
    • Place of Presentation
      東京
    • Year and Date
      20110421-22
  • [Presentation] Effect of Lactobacillus spp. on colonization of H. pylori in gastric mucosa of mongolian gerbil2011

    • Author(s)
      Zaman C, Osaki T, 他4名
    • Organizer
      第17回日本ヘリコバクター学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      2011-06-24
  • [Presentation] Helicobacter pylori感染に及ぼす口腔内細菌の影響2011

    • Author(s)
      米澤英雄, 大崎敬子, 他3名
    • Organizer
      第17回日本ヘリコバクター学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      2011-06-24
  • [Presentation] Prevalence of Helicobacter pylori infection in Japanese children and their Family-sasayama study-2011

    • Author(s)
      Okuda M, Kikuchi S, Osaki T, 他6名
    • Organizer
      The 8 th Japan-Korea Joint Symposium on Helicobacter infection
    • Place of Presentation
      Seoul
    • Year and Date
      2011-05-28
  • [Presentation] 本邦のHelicobacter pylori感染における環境由来感染の可能性について2011

    • Author(s)
      大崎敬子, 他4名
    • Organizer
      第15回日本小児H. pylori研究会
    • Place of Presentation
      滋賀
    • Year and Date
      2011-03-05
  • [Presentation] Mutual interaction between Helicobacter pylori and oral bacteria2010

    • Author(s)
      Yonezawa H, Osaki T, Kamiya S
    • Organizer
      45th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel
    • Place of Presentation
      Kyoto
    • Year and Date
      20101206-08
  • [Presentation] Biofilm formation by Helicobacter pylori and its pathogenesis2010

    • Author(s)
      Kamiya S, Yonezawa H, Woo T, Kurata S, Zaman C, Hanawa T, Kato S & Osaki T
    • Organizer
      The 33rd International Congress on Microbial Ecology in health and Disease
    • Place of Presentation
      Greece
    • Year and Date
      20100906-10
  • [Presentation] 鉄欠乏性貧血患者より分離されたHelicobacter pyloriの鉄利用能と関連遺伝子群の解析2010

    • Author(s)
      大崎敬子, 加藤晴一, 他3名
    • Organizer
      第16回日本ヘリコバクター学会総会
    • Place of Presentation
      京都
    • Year and Date
      20100624-25
  • [Presentation] Helicobacter pyloriのバイオフィルムにおける遺伝子発現制御の解析2010

    • Author(s)
      米澤英雄, 大崎敬子, 他3名
    • Organizer
      第16回日本ヘリコバクター学会総会
    • Place of Presentation
      京都
    • Year and Date
      20100624-25
  • [Presentation] 鉄欠乏性貧血患者より分離されたHelicobacter pyloriの性状解析2010

    • Author(s)
      大崎敬子, 加藤晴一, 他2名
    • Organizer
      第84回日本感染症学会総会
    • Place of Presentation
      京都
    • Year and Date
      20100405-06
  • [Presentation] 鉄欠乏性貧血由来Helicobacter pyloriのDNAマイクロアレイ法による解析2010

    • Author(s)
      大崎敬子, 加藤晴一, 他6名
    • Organizer
      第83回日本細菌学会
    • Place of Presentation
      横浜
    • Year and Date
      20100327-29
  • [Presentation] Growth inhibition of Helicobacter pylori by gastric microflora of Mongolian gerbils2010

    • Author(s)
      Zaman C, Osaki T, 他4名
    • Organizer
      The 10th Japan-Korea International Symposium on Microbiology
    • Place of Presentation
      Yokohama
    • Year and Date
      20100325-26
  • [Presentation] Mutual correlation between gastric flora and Helicobacter pylori gastric mucosa of Mongolian gerbil2010

    • Author(s)
      Zaman C, Osaki T, 他4名
    • Organizer
      第43回日本無菌生物ノートバイオロジー学会総会
    • Place of Presentation
      東京
    • Year and Date
      20100121-22
  • [Presentation] Helicobacter pyloriの環境中での生存条件についての検討2010

    • Author(s)
      大崎敬子、米澤英雄、, 他4名
    • Organizer
      第45回日本無菌生物ノートバイオロジー学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      20100112-13
  • [Presentation] Helicobacter pyloriのバイオフィルム形成におけるCsrAの機能解析2010

    • Author(s)
      米澤英雄, 大崎敬子, 他5名
    • Organizer
      第24回Bactrial Adherence & Biofilm学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-09
  • [Presentation] Helicobacter pylori感染スナネズミにおける抗H. pylori作用を示す胃内細菌叢の解析2010

    • Author(s)
      大崎敬子, Cynthia Zaman, 神谷茂
    • Organizer
      小児ヘリコバクター研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-13
  • [Presentation] Helicobacter pylori TK1402株バイオフィルム形成が及ぼすウレアーゼ発現機構の解析2009

    • Author(s)
      米澤英雄, 大崎敬子, 他3名
    • Organizer
      第92回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      20091105-06
  • [Presentation] The role of quorum sensing in Helicobacter pylori-Nickel iron uptake and outer membrane proteins2009

    • Author(s)
      Osaki T, Yonezawa H, 他4名
    • Organizer
      15th International Workshop on Campylobacter, Helicobacter, and Related Organisms
    • Place of Presentation
      Niigata
    • Year and Date
      20090902-05
  • [Presentation] Biofilm forming ability of Helicobacter pylori strain TK1402 and its role in pathogenesis2009

    • Author(s)
      Yonezawa H, Osaki T, 他4名
    • Organizer
      15th International Workshop on Campylobacter, Helicobacter, and Related Organisms
    • Place of Presentation
      Niigata
    • Year and Date
      20090902-05
  • [Presentation] Helicobacter pylori luxS変異株におけるニッケル取り込みと外膜蛋白の構成について2009

    • Author(s)
      大崎敬子, 米澤英雄, 他5名
    • Organizer
      第15回日本ヘリコバクター学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      20090625-26
  • [Presentation] スナネズミ胃正常細菌叢の解析とそれらのHelicobacter pyloriとの相互作用2009

    • Author(s)
      Cynthia Zaman, 大崎敬子, 他4名
    • Organizer
      第15回日本ヘリコバクター学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      20090625-26
  • [Presentation] Helicobacter pylori TK1402株バイオフィルム形成におけるウレアーゼの発現の解析2009

    • Author(s)
      米澤英雄, 大崎敬子, 他4名
    • Organizer
      第15回日本ヘリコバクター学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      20090625-26
  • [Presentation] Helicobacter pylori感染に抑制作用を示す細菌叢の解析2009

    • Author(s)
      大崎敬子, 蔵田訓, 他3名
    • Organizer
      第83回日本感染症学会総会
    • Place of Presentation
      東京
    • Year and Date
      20090423-24
  • [Presentation] Helicobacter pyloriバイオフィルム形成によるクラリスロマイシン処理への影響2009

    • Author(s)
      米澤英雄, 大崎敬子, 他4名
    • Organizer
      第38回杏林医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-21

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Published: 2013-07-31  

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