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2011 Fiscal Year Final Research Report

Subunit vaccine for Tbc with mucosal adjuvant activity of LT

Research Project

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Project/Area Number 21590497
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionFujita Health University

Principal Investigator

TSUJI Takao  藤田保健衛生大学, 医学部, 教授 (60171998)

Project Period (FY) 2009 – 2011
Keywords結核抗原 / 毒素原性大腸菌 / 下痢毒素(LT) / Ag85A, B, C / CFP-10
Research Abstract

Ag85A, B or C of BCG and CFP-10, EAST-6, PPE or TB7.3 of Tbc were overproduced and purified. Although TB7.3 was aggregated and could not be purified, the others were purified and used for antigens to immunize mice with mutant LT as the mucosal adjuvant. We determined that the mice immunized with Ag85A, B or C plus mLT were not effective for prevention of BCG or Tbc infection. As we need much time to determine whether CFP-10, EAST-6, PP E or TB7.3 from Tbc will be effective to Tbc infection as the vaccine, we are doing some experiments to determine it.

  • Research Products

    (21 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (8 results) Book (1 results)

  • [Journal Article] Specific egg yolk immunoglobulin as a new preventive approach for Shiga-toxin-mediated diseases2012

    • Author(s)
      Neri P, Tokoro S, Kobayashi R, Sugiyama T, Umeda K, Shimizu T, Tsuji T, Kodama Y, Oguma K, Mori H.
    • Journal Title

      PLoS One

      Volume: 6(10) Pages: e26526

    • Peer Reviewed
  • [Journal Article] Randomized placebo-controlled clinical trial of immunoglobulin Y as adjunct to standard supportive therapy for rotavirus-associated diarrhea among pediatric patients.2012

    • Author(s)
      Rahman S, Higo-Moriguchi K, Htun KW, Taniguchi K, Icatlo FC Jr, Tsuji T, Kodama Y, Van Nguyen S, Umeda K, Oo HN, Myint YY, Htut T, Myint SS, Thura K, Thu HM, Fatmawati NN, Oguma K.
    • Journal Title

      Vaccine

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Recombinant Shiga toxin B subunit can induce neutralizing IgY antibody Biol2012

    • Author(s)
      Neri P, Tokoro S, Sugiyama T, Umeda K, Shimizu T, Tsuji T, Kodama Y, Mori H.
    • Journal Title

      Pharm. Bull

      Volume: 35(6) Pages: 917-923

    • Peer Reviewed
  • [Journal Article] Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water2011

    • Author(s)
      Ribeiro AD, Niemann FS, Gatti MSV, Lanna MSC, Tsuji T, Yano T.
    • Journal Title

      J Vennom Anim Toxins and Trop Dis

      Volume: 17 Pages: 103-107

    • Peer Reviewed
  • [Journal Article] The bacteriological and epidemiological characteristics of enterotoxigenic Escherichia coli isolated in Tokyo between 1966 and 20092011

    • Author(s)
      Koshi N., Obata H., Monma C., Nakama A., Kai A., Tsuji T.
    • Journal Title

      J. Clinical. Microbiol

      Volume: 49 Pages: 3348-3351

    • Peer Reviewed
  • [Journal Article] Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin : insight into the ganglioside binding mechanism2011

    • Author(s)
      Nuemket N, Tanaka Y, Tsukamoto K, Tsuji T Nakamura K, Kozaki S, Yao M, Tanaka I.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 411(2) Pages: 433-9

    • Peer Reviewed
  • [Journal Article] Single chain variable fragment antibodies against Shiga toxins isolated from a human antibody phage display library2011

    • Author(s)
      Neri P, Shigemori N, Hamada-Tsutsumi S, Tsukamoto K, Arimitsu H, Shimizu T, Akahori Y, Kurosawa Y, Tsuji T.
    • Journal Title

      Vaccine

      Volume: 29(33) Pages: 5340-6

    • Peer Reviewed
  • [Journal Article] Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum2010

    • Author(s)
      Nuemket N, Tanaka Y, Tsukamoto K, Tsuji T Nakamura K, Kozaki S, Yao M, Tanaka I.
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 66 Pages: 608-10

    • Peer Reviewed
  • [Journal Article] Passive oral immunization by egg yolk immunoglobulin(IgY) to Vibrio cholerae effectively prevents cholera2010

    • Author(s)
      Hirai K, Arimitsu H, Umeda K, Yokota K, Shen L, Ayada K, Kodama Y, Tsuji T, Hirai Y, Oguma K.
    • Journal Title

      Acta Med Okayama

      Volume: 64(3) Pages: 163-70

    • Peer Reviewed
  • [Journal Article] Lincomycin-induced over-expression of mature recombinant cholera toxin B subunit and the holotoxin in Escherichia coli2009

    • Author(s)
      Arimitsu H, Tsukamoto K, Ochi S, Sasaki K, Kato M, Taniguchi K, Oguma K and Tsuji T.
    • Journal Title

      Protein Expression and Purification

      Volume: 67 Pages: 96-103

    • Peer Reviewed
  • [Journal Article] Nucleotide sequence analysis of the enterotoxigenic Escherichia col i Ent plasmid2009

    • Author(s)
      Ochi S, Shimizu T, Ohtani K, Ichinose Y, Arimitsu H, Tsukamoto K, Kato M, Tsuji T.
    • Journal Title

      DNA Res

      Volume: 16(5) Pages: 299-309

    • Peer Reviewed
  • [Journal Article] 6種類の毒素原性大腸菌が検出された仕出し弁当を原因とする集団食中毒例とColony-sweep PCR法を応用した検査法について2009

    • Author(s)
      小西典子、尾畑浩魅、下島優香子、門間千枝、甲斐明美、辻孝雄
    • Journal Title

      感染症学雑誌

      Volume: 83(5) Pages: 490-495

    • Peer Reviewed
  • [Presentation] 胚性腫瘍細胞P19を用いたボツリヌス神経毒素の作用機構の解析2012

    • Author(s)
      塚本健太郎、辻孝雄
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎県長崎市
    • Year and Date
      2012-03-29
  • [Presentation] Structure and activity of receptor binding domain of Clostridium botulinum D/C mosaic neurotoxin2011

    • Author(s)
      Kentaro Tsukamoto, Takao Tsuji
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-07
  • [Presentation] ボツリヌスC型及びDCモザイク神経毒素は異なるガングリオシドを介して細胞内に侵入する2010

    • Author(s)
      西脇啓太、辻孝雄
    • Organizer
      第47回日本細菌学会中部支部総会
    • Place of Presentation
      新潟県新潟市
    • Year and Date
      2010-10-23
  • [Presentation] ボツリヌス神経毒素受容体結合領域の結晶構造と細胞内侵入機構の解析2010

    • Author(s)
      塚本健太郎、辻孝雄
    • Organizer
      第57回トキシンシンポジウム
    • Place of Presentation
      滋賀県長浜市
    • Year and Date
      2010-07-15
  • [Presentation] ボツリヌスDCモザイク毒素重鎖C末端領域の構造と活性について2010

    • Author(s)
      塚本健太郎、辻孝雄
    • Organizer
      第8回感染症沖縄フォーラム
    • Place of Presentation
      沖縄県宜野湾市
    • Year and Date
      2010-02-12
  • [Presentation] EC細胞を用いたボツリヌス神経毒素重鎖C末端領域の機能解析2009

    • Author(s)
      塚本健太郎、辻孝雄
    • Organizer
      第46回日本細菌学会中部支部総会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2009-10-23
  • [Presentation] ボツリヌス神経毒素の脂質受容体に対する結合活性の解析2009

    • Author(s)
      塚本健太郎、辻孝雄
    • Organizer
      第41回藤田学園医学会
    • Place of Presentation
      愛知県豊明市
    • Year and Date
      2009-10-02
  • [Presentation] ボツリヌス神経毒素重鎖C末端領域の脂質受容体に対する結合活性2009

    • Author(s)
      塚本健太郎、辻孝雄
    • Organizer
      第56回トキシンシンポジウム
    • Place of Presentation
      岐阜県岐阜市
    • Year and Date
      2009-08-26
  • [Book] ナイセリア科とその類縁属の菌について、シンプル微生物学、(第5版)2011

    • Author(s)
      辻孝雄
    • Total Pages
      159-166

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Published: 2013-07-31  

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