2011 Fiscal Year Final Research Report
Study of Clostridium perfringens beta-tox in : binding to lipid-rafts and effect on signal transduction.
| Project/Area Number |
21590500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Keiko (90170315)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ウエルシュ菌 / β毒素 / THP-1細胞 / マイクロドメイン / ネクローシス |
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| Research Abstract |
Incubation of THP-l cells with C. perfringens beta-toxin showed the formation of oligomer on lipid rafts. Beta-toxin induced the activation of endogenous phospholipase C and production of IP_3.IP_3-induced Ca^<2+> uptake caused the activation of calmodulin-calcineurin signaling pathway. As beta-toxin caused PI entry and cell swelling, beta-toxin produced cytotoxic activity through necrosis.
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