2011 Fiscal Year Final Research Report
The use of novel humanized mouse model for the study of immunotherapy against HTLV-1 infection
| Project/Area Number |
21590512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
SAITO Mineki 琉球大学, 大学院・医学研究科, 准教授 (40398285)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yuetsu 琉球大学, 大学院・医学研究科, 教授 (30163588)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 感染防御 / ワクチン / ヒト化マウス / HTLV-1 |
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| Research Abstract |
In this study, HTLV-1-negative normal human peripheral blood mononuclear cells(PBMCs) were transplanted directly into the spleens of NOD/SCID/γCnull(NOG) mice together with mitomycin treated HTLV-1-infected cell line. We demonstrated that the both human CD4 and CD8 positive T cells were infected with HTLV-1, and that Tax expression was induced by short time(6hrs) in vitro cultivation, as observed in naturally infected T-cells. Moreover, viral gene expression was also very similar to those observed in naturally infected T-cells. Our method provides a simple, rapid and effective approach for assessment of HTLV-1 infection in vivo ; it is therefore useful in the study of infection, pathogenesis and immunotherapies.
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