Pathology of Merkel cell polyomavirus

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KATANO Harutaka 国立感染症研究所, 感染病理部, 室長 (70321867)
• Co-Investigator(Renkei-kenkyūsha): SATA Tetsutaro 国立感染症研究所, 感染病理部, 部長 (00162397) ; SATO Yuko 国立感染症研究所, 感染病理部, 主任研究官 ; FUKUMOTO Hitomi 国立感染症研究所, 感染病理部, 協力研究員 ; KANNO Akayuki 国立感染症研究所, 感染病理部, 主任研究官 (50272563) ; NAKAMURA Tomoyuki 国立感染症研究所, 感染病理部, 研究生 ; SAKAMOTO Kouta 国立感染症研究所, 感染病理部, 研究生 ; TAKENOUCHI Erika 国立感染症研究所, 感染病理部, 研究生
• Project Period (FY): 2009 – 2011
• Keywords: 病原性

Research Abstract

Merkel cell polyomavirus-DNA was detected in 6 of 11(55%) Japanese cases of Merkel cell carcinoma by real-time PCR. Immunohistochemistry using a rabbit polyclonal antibody revealed that large T antigen was expressed in the nuclei of Merkel cell carcinoma cells. Deletion mutant analyses identified a nuclear localization signal in large T antigen. Real-time PCR analysis revealed that Merkel cell polyomavirus replication is associated with host immunity, and that circulation of human polyomavirus-6 and-7 in the serum is rare.

Research Products

• [Journal Article] Metachronous merkel cell carcinoma on both cheeks (2012)
  • Author(s): Kamiyama T, Ohshima N, Satoh H, Fukumoto H, Katano H, Imakado S
  • Journal Title: Acta Derm Venereol Volume: 92 Pages: 54-56
  • DOI: doi:10.2340/00015555-1185
• [Journal Article] Pathology of Kaposi's Sarcoma-Associated Herpesvirus Infection (2011)
  • Author(s): Fukumoto H, Kanno T, Hasegawa H, Katano H
  • Journal Title: Front Microbiol Volume: 175 Pages: 1-9
  • DOI: doi:10.3389/fmicb.2011.00175
• [Journal Article] A novel real-time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses (2011)
  • Author(s): Katano H, Kano M, Nakamura T, Kanno T, Asanuma H, Sata T
  • Journal Title: J Med Virol Volume: 83 Pages: 322-330
  • DOI: doi:10.1002/jmv.21962
• [Journal Article] Detection of Merkel cell polyomavirus in cutaneous squamous cell carcinoma before occurrence of Merkel cell carcinoma (2011)
  • Author(s): Kaibuchi-Noda K, Yokota K, Matsumoto T, Sawada M, Sakakibara A, Kono M, Tomita Y, Watanabe D, Fukumoto H, Katano H, Akiyama M
  • Journal Title: J Am Acad Dermatol Volume: 65 Pages: e152-154
  • DOI: doi:10.1016/j.jaad.2011.06.026
• [Journal Article] Nuclear localization of Merkel cell polyomavirus large T antigen in Merkel cell carcinoma (2010)
  • Author(s): Nakamura T, Sato Y, Watanabe D, Ito H, Shimonohara N, Tsuji T, Nakajima N, Suzuki Y, Matsuo K, Nakagawa H, Sata T, Katano H
  • Journal Title: Virology Volume: 398 Pages: 273-279
  • DOI: doi:10.1016/j.virol.2009.12.024
• [Journal Article] ポリオーマウイルス感染と病態-メルケル細胞ポリオーマウイルスを中心に (2010)
  • Author(s): 片野晴隆
  • Journal Title: 化学療法の領域 Volume: 26 Pages: 1251-1258
  • URL: https://www.iyaku-j.com/index.php?main_page=index & cPath=5_1_16_3279
• [Journal Article] Detection of Merkel cell polyomavirus in Merkel cell carcinoma and Kaposi's sarcoma (2009)
  • Author(s): Katano H, Ito H, Suzuki Y, Nakamura T, Sato Y, Tsuji T, Matsuo K, Nakagawa H, Sata T
  • Journal Title: J Med Virol Volume: 81 Pages: 1951-1958
  • DOI: doi:10.1002/jmv.21608
• [Journal Article] メルケル細胞ポリオーマウイルスとメルケル細胞癌 (2009)
  • Author(s): 中村智之、片野晴隆
  • Journal Title: ウイルス Volume: 59 Pages: 37-42
  • DOI: doi:10.2222/jsv.59.37
• [Presentation] メルケル細胞ポリオーマウイルスlarge T抗原の核発現 (2010)
  • Author(s): 片野晴隆、中島典子、辻隆裕、鈴木良夫、佐多徹太郎
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-29
• [Presentation] メルケル細胞ポリオーマウイルスlarge T抗原の核発現と発癌の関連 (2009)
  • Author(s): 中村智之、佐藤由子、渡辺大輔、伊東秀記、下ノ原望、辻隆裕、中島典子、鈴木良夫、松尾光馬、中川秀巳、小島直也、佐多徹太郎、片野晴隆
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Remarks]
  • URL: http://www0.nih.go.jp/niid/pathology/MCPyV.html