Pathogenesis of tissue remodeling caused by endogenous AGE and advanced targeting therapy

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590604
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: Shujitsu University
• Principal Investigator: MORI Shuji 就実大学, 薬学部, 教授 (50220009)
• Co-Investigator(Kenkyū-buntansha): GOMITA Yutaka 就実大学, 薬学部, 教授 (00088709)
• Project Period (FY): 2009 – 2011
• Keywords: 臨床薬理学 / 組織リモデリング / 分子標的薬

Research Abstract

The protein array analysis with endogenous AGE as specific probe showed some high affinity binding factors for endogenous AGE, suggesting the formation of pathophysiological cluster in the local site of inflammatory. In addition, we succeeded the establishment of in vitro binding assay between recombinant endogenous AGE and RAGE. Also, Cell-based assay upon NF-kB activation and proinflammatory cytokine production was developed, providing the convenient platform for exploration of specific antagonist to endogenous AGE.

Research Products

• [Journal Article] Anti-high mobility group box-1 monoclonal antibody protects the blood-brain barrier from ischemia-induced disruption in rats (2011)
  • Author(s): Zhang J, Takahashi HK, Liu K, Wake H, Liu R, Maruo T, Date I, Yoshino T, Ohtsuka A, Mori S, Nishibori M
  • Journal Title: Stroke Volume: 42 Pages: 1420-1428
  • DOI: DOI:10.1161/STROKEAHA.110.598334
  • Peer Reviewed
• [Journal Article] High-mobility group box protein 1 neutralization reduces development of diet-induced atherosclerosis in apolipoprotein e-deficient mice (2011)
  • Author(s): Kanellakis P, Agrotis A, Kyaw TS, Koulis C, Ahrens I, Mori S, Takahashi HK, Liu K, Peter K, Nishibori M, Bobik A
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 31 Pages: 313-319
  • DOI: DOI:10.1161/ATVBAHA.110.218669
  • Peer Reviewed
• [Journal Article] Ciprofloxacin inhibits advanced glycation end products-induced adhesion molecule expression on human monocytes (2010)
  • Author(s): Mori S, Takahashi HK, Liu K, Wake H, Zhang J, Liu R, Yoshino T, Nishibori M
  • Journal Title: Br J Pharmacol Volume: 161 Pages: 229-240
  • DOI: DOI:10.1111/j.1476-5381.2010.00880.x
  • Peer Reviewed
• [Journal Article] Effect of nicotine on advanced glycation end product-induced immune response in human monocytes (2010)
  • Author(s): Takahashi HK, Liu K, Wake H, Mori S, Zhang J, Liu R, Yoshino T, Nishibori M
  • Journal Title: J Pharmacol Exp Ther Volume: 332 Pages: 1013-1021
  • DOI: DOI:10.1124/jpet.109.160861
  • Peer Reviewed
• [Journal Article] Prostaglandin E_2 inhibits advanced glycation end product-induced adhesion molecule expression on monocytes, cytokine production, and lymphocyte proliferation during human mixed lymphocyte reaction (2010)
  • Author(s): Takahashi HK, Zhang J, Mori S, Liu K, Wake H, Liu R, Sadamori H, Matsuda H, Yagi T, Yoshino T, Nishibori M
  • Journal Title: J Pharmacol Exp Ther Volume: 334 Pages: 964-972
  • DOI: DOI:10.1124/jpet.110.169102
  • Peer Reviewed
• [Journal Article] Prostaglandin E2 inhibits advanced glycation end product-induced adhesion molecule expression, cytokine production, and lymphocyte proliferation in human peripheral blood mononuclear cells (2009)
  • Author(s): Takahashi HK, Liu K, Wake H, Mori S, Zhang J, Liu R, Yoshino T, Nishibori M
  • Journal Title: J Pharmacol Exp Ther Volume: 331 Pages: 656-670
  • DOI: DOI:10.1124/jpet.109.157594
  • Peer Reviewed
• [Journal Article] Histamine inhibits advanced glycation end products-induced adhesion molecule expression on human monocytes (2009)
  • Author(s): Wake H, Takahashi HK, Mori S, Liu K, Yoshino T, Nishibori M
  • Journal Title: J Pharmacol Exp Ther Volume: 330 Pages: 826-833
  • DOI: DOI:10.1124/jpet.109.155960
  • Peer Reviewed
• [Journal Article] Advanced glycation end products subspecies-selectively induce adhesion molecule expression and cytokine production in human peripheral blood mononuclear cells (2009)
  • Author(s): Takahashi HK, Mori S, Wake H, Liu K, Yoshino T, Ohashi K, Tanaka N, Shikata K, Makino H, Nishibori M
  • Journal Title: J Pharmacol Exp Ther Volume: 330 Pages: 89-98
  • DOI: DOI:10.1124/jpet.109.150581
  • Peer Reviewed
• [Presentation] アレイ解析によるHMGB1相互作用因子の網羅的探索 (2012)
  • Author(s): 森秀治
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 20120300
• [Presentation] 治療分子標的としてのHMGB1と創薬 (2012)
  • Author(s): 森秀治
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 20120300
• [Presentation] 抗HMGB1抗体の結合特異性と機能解析 (2010)
  • Author(s): 森秀治
  • Organizer: 第49回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 米子
  • Year and Date: 20101100