2011 Fiscal Year Final Research Report
Development of a novel, rapid, easy and affordable identification IT system for pathogens in sepsis
| Project/Area Number |
21590612
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
NIIMI Hideki 富山大学, 大学病院, 助教 (50401865)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Isao 富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NOTE Ryougou 富山大学, 大学病院, 主任臨床検査技師 (60377364)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 遺伝子検査学 |
|---|
| Research Abstract |
To detect pathogenic bacteria by PCR sensitively and correctly, we newly developed "eukaryote-made" Taq polymerase, which is free from bacterial DNA contamination(J Clin Microbiol. 2011 Sep ; 49(9): 3316-20). We also developed a novel rapid method to identify pathogenic microorganisms using 7 primer sets, real-time PCR, high resolution melting analysis(RotorGeneQ : QIAGEN), and the original web-based identification software(International patent application : 2007). To combine the eukaryote-made Taq polymerase and a novel identification method, we developed a rapid identification system of pathogenic microorganisms within 3 hours after collection of patient samples.
|
