2011 Fiscal Year Final Research Report
Fecal-based long DNA assay for colorectal cancer detection
| Project/Area Number |
21590626
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
SUEHIRO Yutaka 山口大学, 大学院・医学系研究科, 准教 (40290978)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 便DNA検査 |
|---|
| Research Abstract |
Purpose : Although the fecal occult blood test is performed daily as a primary screening test, sensitivity is low, and false positive reactions may occur when hemorrhoids are present.
Fecal DNA analysis appears to be a valid alternative method providing more advanced and reliable detection of CRC. Thus, we evaluated whether the DNA test could improve diagnostic performance for CRC detection.
Methods : DNA was isolated from stools of patients with CRC and Twist1 methylation level was evaluated using pyrosequencing.
Results : We found TWIST1 methylation occurred in feces with a sensitivity of 18% for detection of CRC.
Conclusions : TWIS1 methylation assay may be useful for the detection of CRC.
|
