2011 Fiscal Year Final Research Report

Quantitative analysis of yeast cell population by using viability-dependent fluorescent dyes and its Clinical application

Research Project

Project/Area Number	21590634
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Laboratory medicine
Research Institution	University of the Ryukyus
Principal Investigator	YAMANE Nobuhisa 琉球大学, 大学院・医学研究科, 教授 (80125682)
Co-Investigator(Kenkyū-buntansha)	SHIOHIRA Chika 琉球大学, 大学院・医学研究科, 助教 (50325833) HIGASHIUESATO Yasushi 琉球大学, 医学部附属病院, 助教 (80381226)
Project Period (FY)	2009 – 2011
Keywords	酵母真菌 / 蛍光プローブ / 生育活性 / 抗真菌薬 / 感染症
Research Abstract	We established a technique to determine the viability of yeast cells in urine specimens by directly staining with fluorescent dyes, which have different permeability into yeast cells. After staining, yeast cells were analyzed by flow cytometer. Yeast cells in urine specimens from competent patients against infection were mostly classified as injured cells, whereas those from compromised patients were live cells. Also, the increased ratios of injured cells were observed along the improvement of clinical manifestation of compromised diseases. This established procedure provides us with a perspective prognostic indicator for a variety of diseases following the compromised against infections.

(3 results)