The study on the developmental neurotoxicity of organotin in rats by analyzing mRNA and protein expressions and behaviors

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590661
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Kitasato University
• Principal Investigator: TSUNODA Masashi 北里大学, 医学部, 准教授 (00271221)
• Co-Investigator(Kenkyū-buntansha): KUDO Yuichiro 北里大学, 医学部, 講師 (60348505) ; KONISHI Yoshiko 国立医薬品食品衛生研究所, 衛生微生物部, 研究員 (10195761) ; TASHIRO Tomoko 青山学院大学, 理工学部, 教授 (50114541)
• Project Period (FY): 2009 – 2011
• Keywords: トリブチルスズ / 発達神経毒性 / 二世代曝露 / 行動学試験 / 遺伝子解析

Research Abstract

The neurotoxic effects of tributyltin(TBT) on the F1 rats were examined. The pregnant rats were exposed to TBT via their food, therefore, the F1 rats were exposed to TBT via the placenta and their dam's milk. After weaning, the half of F1 rats were exposed to TBT via their food. The inhibitory effects of behaviors in the F1 rats exposed to TBT via the placenta and their dam's milk were observed as well as the decrease in their body weight compared to the control. The F1 rats exposed to TBT via their food after weaning(at 3 weeks of age) also showed lower activities and body weight compared to the control. The exposure to TBT just after the weaning is toxic as the exposure via the placenta and their dam's milk. The inhibitory effects on the behaviors by TBT may be related to dopamine metabolism, since tyrosine hydorxylase, dopamine transporter and dopamine receptor D2 in the midbrain were down-regulated.

Research Products

• [Journal Article] Evaluation of neurotoxicity of artificial dura mater containing a high concentration of dibutyltin in rats after intracranial implantation (2012)
  • Author(s): Tsunoda, M., Ikeuchi, R., Tsuji, M., Inoue, Y., Ito, K., Katagiri, H., Akita, H., Saji, M., Yuba, T., Yamada, T., Tsuchiya, T. and Aizawa, Y
  • Journal Title: The Kitasato Medical Journal Volume: 42 Pages: 67-75
• [Journal Article] Exposure to tributyltin(TBT) via food during developmental stages after weaning, or the exposure via the placenta and their dams' milk, inhibits body weight gain and behaviors in rats (2012)
  • Author(s): Ikeuchi, R., Kido, T., Sugaya, C., Katagiri, H., Akita, H., Saji, M., Tsunoda, M. and Aizawa, Y
  • Journal Title: The Kitasato Medical Journal Volume: 42 Pages: 57-66
• [Journal Article] Higher toxicity of dibutyltin and poly-L-lactide with a large amount of tin but lower toxicity of poly-L-lactide of synthetic artificial dura mater exhibited on murine astrocyte cell line (2010)
  • Author(s): Tsuji, M., Inoue, Y., Sugaya, C., Tsunoda, M., Sugaya, T., Takahashi, M., Yuba, T., Tsuchiya, T. and Aizawa, Y
  • Journal Title: YAKUGAKU ZASSHI Volume: 130 Pages: 847-855
• [Journal Article] Enhanced inhibitory effects of TBT chloride on the development of F1 rats (2010)
  • Author(s): Asakawa, H., Tsunoda, M., Kaido, T., Hosokawa, M., Sugaya, C., Inoue, Y., Kudo, Y., Satoh, T., Katagiri, H., Akita, H., Saji, M., Wakasa, M., Negishi, T., Tashiro, T. and Aizawa Y
  • Journal Title: Archives of Environmental Toxicology and Contamination Volume: 58 Pages: 1065-1073
• [Presentation] The immunotoxic effects of tributyltin on splenocytes in female F1 rats by continuous exposure from the fetus stage through their developing stages (2012)
  • Author(s): Ikeuchi, R., Kido, T., Sugaya, C., Tsunoda, M., Katagiri, H., Hayashi, K., Kosaka, T. and Aizawa, Y
  • Organizer: 51st Annual Meeting, Society of Toxicology
  • Place of Presentation: 米国サンフランシスコ市
  • Year and Date: 2012-03-14
• [Presentation] The effects of tributyltin on the bones of F1 rats by exposure via the placenta and their dams' milk and/or food (2012)
  • Author(s): Hosokawa, M., Tsunoda, M., Asakawa, H., Kaido, T., Sugaya, C., Inoue, Y., Tashiro, T., Yokoyama, K. and Aizawa, Y
  • Organizer: 51st Annual Meeting, Society of Toxicology
  • Place of Presentation: 米国サンフランシスコ市
  • Year and Date: 2012-03-14
• [Presentation] 人工硬膜の主成分であるポリ乳酸ラクチドのアストロサイト系細胞増殖能への影響 (2011)
  • Author(s): 辻雅善、井上葉子、菅谷ちえ美、角田正史、菅谷津貴子、高橋正身、柚場俊康、土屋利江、相澤好治、福島哲仁
  • Year and Date: 20110300
• [Presentation] トリブチルスズに胎児期-発達初期曝露されたオスF1ラットの脾細胞サイトカイン産生に関する検討 (2011)
  • Author(s): 角田正史、木戸尊將、池内龍太郎、菅谷ちえ美、片桐裕史、相澤好治
  • Organizer: 第18回免疫毒性学会学術大会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-09-09
• [Presentation] 合成吸収性人工硬膜の安全性評価:動物モデルの1か月観察後の脳各部位神経伝達物質測定 (2011)
  • Author(s): 池内龍太郎、峡戸孝也、細川まゆ子、菅谷ちえ美、井上葉子、角田正史、片桐裕史、土屋利江、相澤好治
  • Organizer: 第22回日本微量元素学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-06-01
• [Presentation] トリブチルスズに胎児期-発達期曝露されたF1ラットの成長抑制及び行動抑制 (2011)
  • Author(s): 池内龍太郎、木戸尊將、菅谷ちえ美、角田正史、片桐裕史、内村彩子、相澤好治
  • Organizer: 第84回日本産業衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-18
• [Presentation] Evaluation of TBT neurotoxicity in developing male F1 rats by open field tests (2011)
  • Author(s): Tsunoda, M., Kaido, T., Ikeuchi, R., Hosokawa, M., Sugaya, C., Inoue, Y., Kudo, Y., Akita, H., Saji, M., Takeuchi, Y., Yoshioka, R., Tashiro, T. and Aizawa Y
  • Organizer: 49th Annual Meeting, Society of Toxicology
  • Place of Presentation: 米国ソルトレーク市
  • Year and Date: 2011-03-10
• [Presentation] Behavior changes and tributyltin(TBT) concentration in cerebrums of pregnant rats exposed to TBT (2011)
  • Author(s): Kaido, T., Asakawa, H., Takeuchi, Y., Yoshioka, R., Ikeuchi, R., Hosokawa, M., Sugaya, C., Inoue, Y., Kudo, Y., Tsunoda, M., Akita, H., Saji, M., Tashiro, T., Ohhira, S. and Aizawa, Y
  • Organizer: 49th Annual Meeting, Society of Toxicology
  • Place of Presentation: 米国ソルトレーク市
  • Year and Date: 2011-03-10
• [Presentation] The neurotoxic effects of tributyltin on male F1 rats by continuous exposure from the fetus stage through their developing stages (2011)
  • Author(s): Ikeuchi, R., Kido, T., Sugaya, C., Tsunoda, M., Katagiri, H., Uchimura, A., Akita, H., Saji, M. and Aizawa, Y
  • Organizer: 50th Annual Meeting, Society of Toxicology
  • Place of Presentation: 米国ワシントンD. C.市
  • Year and Date: 2011-03-08
• [Presentation] The effects of tributyltin(TBT) on splenocytes in F1 rats exposed to TBT via placenta, their dam's milk and/or food (2011)
  • Author(s): Tsunoda, M., Kido, T., Ikeuchi, R., Kaido, T., Hosokawa, M., Sugaya, C., Ohta, H., Katagiri, H., Hayashi, K., Kosaka, T., Takeuchi, Y., Tashiro, T. and Aizawa, Y
  • Organizer: 50th Annual Meeting, Society of Toxicology
  • Place of Presentation: 米国ワシントンD. C.市
  • Year and Date: 2011-03-07
• [Presentation] トリブチルスズ経胎盤・経母乳曝露によるメスF1ラット6週令脾臓リンパ球サブセットの変化 (2010)
  • Author(s): 角田正史、木戸尊將、池内龍太郎、峡戸孝也、細川まゆ子、菅谷ちえ美、工藤雄一朗、林宏一、小坂忠司、竹内裕紀、田代朋子、相澤好治
  • Organizer: 第17回日本免疫毒性学会学術大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-09-10
• [Presentation] Peripheral exposure affects the sensitivity to TBT neurotoxicity after maturation : evaluation by gene expression and behavior analyses (2010)
  • Author(s): Tsunoda, M., Takeuchi, Y., Yoshioka, R., Negishi, T, Ikeuchi, R., Kaido, T., Hosokawa, M., Sugaya. C., Tashiro, T. and Aizawa, Y
  • Organizer: Neuro2010第53回日本神経化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-04
• [Presentation] トリブチルスズ経胎盤・経母乳曝露によるF1ラット6週令時の胸腺リンパ球サブセットの変化 (2010)
  • Author(s): 角田正史、池内龍太郎、峡戸孝也、細川まゆ子、菅谷ちえ美、工藤雄一朗、林宏一、小坂忠司、竹内裕紀、田代朋子、相澤好治
  • Organizer: 第41回日本職業・環境アレルギー学会総会
  • Place of Presentation: 高崎市
  • Year and Date: 2010-07-17
• [Presentation] トリブチルスズ化合物の亜慢性経口曝露による妊娠ラットの脳各部位の神経伝達物質及び体重の変化 (2010)
  • Author(s): 池内龍太郎、峡戸孝也、細川まゆ子、菅谷ちえ美、井上葉子、工藤雄一朗、角田正史、竹内裕紀、田代朋子、相澤好治
  • Organizer: 第21回日本微量元素学会
  • Place of Presentation: 京都市
  • Year and Date: 2010-07-03
• [Presentation] 合成吸収性人工硬膜の安全性評価に関する研究:動物モデルの2ヶ月観察後の脳各部位神経伝達物質測定 (2010)
  • Author(s): 角田正史、峡戸孝也、池内龍太郎、細川まゆ子、工藤雄一朗、土屋利江、相澤好治
  • Organizer: 第21回日本微量元素学会
  • Place of Presentation: 京都市
  • Year and Date: 2010-07-03
• [Presentation] トリブチルスズ(TBT)二世代曝露によるオスF 1ラットの行動に対する影響:オープンフィールド試験による評価 (2010)
  • Author(s): 角田正史、池内龍太郎、峡戸孝也、細川まゆ子、井上葉子、工藤雄一朗、片桐裕史、田代朋子、相澤好治
  • Organizer: 第83回日本産業衛生学会
  • Place of Presentation: 福井市
  • Year and Date: 2010-05-28
• [Presentation] トリブチルスズ二世代曝露(経胎盤経母乳・再曝露)による神経伝達物質の変化 (2010)
  • Author(s): 角田正史、浅川秀雄、池内龍太郎、峡戸孝也、細川まゆ子、菅谷ちえ美、井上葉子、辻雅善、工藤雄一朗、相澤好治
  • Organizer: 第80回日本衛生学会学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-05-10
• [Presentation] トリブチルスズ二世代曝露の6週令、9週令のF1ラットに対するprepulse inhibition test (2010)
  • Author(s): 池内龍太郎、峡戸孝也、細川まゆ子、菅谷ちえ美、井上葉子、角田正史、片桐裕史、秋田久直、佐治眞理、相澤好治
  • Organizer: 第80回日本衛生学会学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-05-10
• [Presentation] Immunotoxic effects of tributyltin on splenocytes and thymocytes in female F1 rats at 9 weeks of age after exposure via the placenta, their dams' milk, and/or their food (2010)
  • Author(s): Tsunoda, M., Ikeuchi, R., Kaido, T., Hosokawa, M., Sugaya, C., Kudo, Y., Hayashi, K., Kosaka, T., Takeuchi, Y., Tashiro, T. and Aizawa Y
  • Organizer: International Symposium on Occupational and Environmental Allergy and Immune Diseases 2010 in Kyoto
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-09
• [Presentation] トリブチルスズ二世代曝露によるF1ラットの骨変化に関する予備的研究 (2009)
  • Author(s): 角田正史、浅川秀雄、峡戸孝也、細川まゆ子、菅谷ちえ美、井上葉子、片桐裕史、工藤雄一朗、根岸隆之、田代朋子、太田久吉、相澤好治
  • Organizer: 第19回日本産業衛生学会産業医・産業看護全国協議会
  • Place of Presentation: 秋田市
  • Year and Date: 2009-11-07
• [Presentation] トリブチルスズ(TBT)化合物の亜慢性経口曝露による妊娠ラット及びF1世代ラットの大脳中TBTとその代謝産物の濃度 (2009)
  • Author(s): 峡戸孝也、細川まゆ子、菅谷ちえ美、井上葉子、角田正史、大平修二、相澤好治
  • Organizer: 第31回日本中毒学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-25
• [Presentation] 合成吸収性人工硬膜の安全性評価に関する研究:動物モデルの2ヶ月観察後の行動学試験 (2009)
  • Author(s): 角田正史、峡戸孝也、細川まゆ子、工藤雄一朗、土屋利江、相澤好治
  • Organizer: 第20回日本微量元素学会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-07-03
• [Presentation] Two-generation toxicogenomic study of the effects of tributyltin on the developing rat brain (2009)
  • Author(s): Tsunoda, M., Wakasa, M., Negishi, T., Kaido, T., Hosokawa, M., Sugaya, C., Inoue, Y., Aizawa, Y. and Tashiro, T
  • Organizer: 第52回日本神経化学会(伊香保)大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-24
• [Presentation] トリブチルスズ二世代曝露によるF1ラットの行動への影響について～6週令での検討 (2009)
  • Author(s): 角田正史、峡戸孝也、細川まゆ子、井上葉子、工藤雄一朗、片桐裕史、田代朋子、相澤好治
  • Organizer: 第82回日本産業衛生学会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-05-22
• [Presentation] トリブチルスズ化合物の亜慢性経口曝露による妊娠ラットの脳各部位の神経伝達物質の変化 (2009)
  • Author(s): 峡戸孝也、細川まゆ子、井上葉子、片桐裕史、工藤雄一朗、角田正史、田代朋子、相澤好治
  • Organizer: 第82回日本産業衛生学会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-05-22