2011 Fiscal Year Final Research Report
DNA damage in human pleural mesothelial cells and lung epithelial cells induced by exposure to carbon nanotubes
| Project/Area Number |
21590670
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | カーボンナノチューブ / 酸化ストレス / 胸膜中皮細胞 / DNA損傷修復 / コメットアッセイ |
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| Research Abstract |
We investigated the potential risk of single-walled carbon nanotube(SWCNT) and multi-walled carbon nanotube(MWCNT) exposure in human pleural mesothelial cells. CNT cytotoxicity was determined using a trypan blue exclusion assay, and DNA damage was detected using an alkaline comet assay. The concentration of 8-oxodeoxyguanosine(8-OHdG) in DNA was measured using HPLC with electrochemical detection. The expression of base excision repair enzymes in the cell was estimated by immunoblot analysis. We observed inhibitory effects on cell proliferation and induction of DNA damage following exposure of cells to purified CNTs that were suspended in dispersion medium. However, accumulation of 8-OHdG in DNA was not found. In addition, the expression levels of base excision enzymes that are involved in hOGG1, hMTH1 and MYH in MeT-5A cells remained unchanged for 24 h after carbon nanotube exposure. CNTs significantly inhibit cell proliferation and decrease DNA damage in human pleural mesothelial cells. Our results indicate that the mechanism of CNT-induced genotoxicity is different from that of exposure to reactive oxygen species, which causes oxidative DNA modifications and 8-OHdG production. Further investigation is required to characterize the specific DNA mutations following CNT exposure.
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