Sensitive detection of hepatitis E virus(HEV) using TaqMan RT-PCR and molecular characterization of HEV in clinical, environmental and putative animal sou rces

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590725
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Hokkaido Institute of Public Health
• Principal Investigator: ISHIDA Setsuko 北海道立衛生研究所, 感染症センター・感染症部, 主査(腸管系ウイルス) (70414315)
• Co-Investigator(Kenkyū-buntansha): YOSHIZUMI Shima 北海道立衛生研究所, 感染症センター・感染症部, 研究職員 (90414317) ; MIYOSHI Masahiro 北海道立衛生研究所, 感染症センター・感染症部, 主査(ウイルス感染症) (50414321) ; KUDO Shinichi 北海道立衛生研究所, 感染症センター・微生物部, 部長 (00414318)
• Co-Investigator(Renkei-kenkyūsha): MATSUBAYASHI Keiji 北海道赤十字血液センター, 検査部, 検査三課長 (90533323) ; GOTO Akiko 北海道立衛生研究所, 感染症センター・感染症部, 研究職員 (60414322)
• Project Period (FY): 2009 – 2011
• Keywords: 疫学 / E型肝炎ウイルス / 診断 / 分子疫学

Research Abstract

A TaqMan RT-PCR was developed to detect and classify genotypes of hepatitis E virus(HEV). Putative animal reservoirs and environmental samples were studied to investigate potential routes of transmission for indigenous HEV infection. HEV RNA was detected using RT-PCR. HEV strains derived from swine liver, environmental samples shared 93-100% sequence similarity with human HEV strains.

Research Products

• [Journal Article] Usefulness of the rapid determination system of viral genome sequences in human stool specimens (2012)
  • Author(s): Miyoshi M, Yoshizumi S, Ishida S, Komagome R, Nagano H, Kudo S, Okano M
  • Journal Title: J Virol Methods Volume: 179 Pages: 256-260
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22123408
  • Peer Reviewed
• [Journal Article] 二枚貝関連の食中毒疑い事例における各種胃腸炎ウイルスの関与-北海道 (2011)
  • Author(s): 吉澄志磨、後藤明子、石田勢津子、野田衛
  • Journal Title: 病原微生物得検出情報 Volume: 32 Pages: 361-363
  • URL: http://idsc.nih.go.jp/iasr/32/382/dj3827.html
• [Journal Article] A Case of Paralytic Poliomyelitis Associated with Poliovirus Vaccine Strains in Hokkaido, Japan (2010)
  • Author(s): Miyoshi M, Yoshizumi S, Jinushi M, Ishida S, Okui T, Okano M, Shouji M, Tanaka S, Saigusa J, Mori A, Tanabe H, Yamaguchi R, Nishimura Y, Shimizu H
  • Journal Title: Jpn J Infect Dis Volume: 63 Pages: 216-217
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20495280
  • Peer Reviewed
• [Journal Article] Different genotypic sapovirusesdetected in two simultaneous outbreaksof gastroenteritis among schoolchildren in the same school district in Hokkaido, Japan (2010)
  • Author(s): Miyoshi M, Yoshizumi S, Kanda N, KarinoT, Nagano H, Kudo S, Okano M, Ishida S
  • Journal Title: Jpn J Infect Dis Volume: 63 Pages: 75-78
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20093770
  • Peer Reviewed
• [Journal Article] Detection of noroviruses in fecal specimens by direct RT-PCR without RNA purification (2010)
  • Author(s): Nishimura N, Nakayama H, Yoshizumi S, Miyoshi M, Tonoike H, Shirasaki Y, Kojima K, Ishida S
  • Journal Title: Journal of Virological Methods Volume: 163 Pages: 282-286
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19878699
  • Peer Reviewed
• [Presentation] 北海道におけるHEV感染の概況について (2011)
  • Author(s): 石田勢津子
  • Organizer: 第29回ウイルス肝炎談話会
  • Place of Presentation: 自治医大(栃木県下野市)
  • Year and Date: 2011-11-19
• [Presentation] 二枚貝の喫食のみられた食中毒疑い事例における各種胃腸炎ウイルスの関与について (2011)
  • Author(s): 吉澄志磨,後藤明子,石田勢津子,田中智之,野田衛
  • Organizer: 第32回日本食品微生物学会学術総会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2011-10-07
• [Presentation] Usefulness of the rapid determination system of viral genome sequences in human stool specimens (2011)
  • Author(s): Miyoshi M, Yoshizumi S, Ishida S, Komagome R, Nagano H, Kudo S, Okano M
  • Organizer: 国際ウイルス学会議(International Union of Microbiological Societies 2011 Congress)
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-15
• [Presentation] Detection and molecular characterization of hepatitis E virus in clinical and environmental samples and in putative animal reservoirs (2011)
  • Author(s): Ishida S, Yoshizumi S, Ikeda T, Miyoshi M, GotoA, Matsubayashi K, IkedaH, Kudo S
  • Organizer: 国際ウイルス学会議(International Union of Microbiological Societies 2011 Congress)
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-13
• [Presentation] 北海道におけるHEV感染の概況について (2011)
  • Author(s): 石田勢津子
  • Organizer: 北海道E型肝炎研究会第5回学術集会
  • Place of Presentation: 札幌医大(札幌市)
  • Year and Date: 2011-09-10
• [Presentation] 糞便直接RT-PCR法によるサポウイルスRNA検出法の開発 (2009)
  • Author(s): 中山博之、吉澄志磨、三好正浩、西村直行、石田勢津子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-10-26