2011 Fiscal Year Final Research Report
Simultaneous analysis of paraquat, glufosinate and organophaophate in biological fluid using solid-phase extraction
| Project/Area Number |
21590745
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Tokai University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | パラコート / グリホサート / 有機リン / 固相抽出 / 一斉分析 |
|---|
| Research Abstract |
We have developed a method based on monolitic spin column extraction and gas chromatography-mass spectrometry(GC-MS) as an analytical method for screening diquat, paraquat, and fenitrothion(MEP) in serum and urine. Then, a rapid, specific, and sensitive method for simultaneous quantitation of organophosphates [MEP, malathion, and phenthoate], glufosinate, and glyphosate in human serum and urine by GC-MS has been developed. Finally, a liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of sixteen organophosphate and carbamate insecticides in serum. Non-polar and polar pesticides were simultaneously extracted from serum samples by a simple and fast monolithic spin column procedure using mix-mode C-C18 cartridges. The validated all method was successfully applied to a clinical poisoning case.
|
-
-
-
-
-
-
-
-
[Presentation] Monolithic spin-column extraction and gas chromatography-mass spectrometry method for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine2010
Author(s)
Saito, T., Namera, A., Yui, Y., Miyazaki, S., Nakamoto, A. and Inokuchi, S
Organizer
The International Association of Forensic Toxcologists 48th International Meeting
Place of Presentation
Bonn Castle(Bonn)
Year and Date
2010-09-30
-
-
