2011 Fiscal Year Final Research Report
microRNA alteration under chronic starvation stress
| Project/Area Number |
21590767
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General internal medicine (including Psychosomatic medicine)
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
TAKAKURA Shu 九州大学, 大学病院, 助教 (40532859)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWAI Keisuke (80325521)
|
|---|
| Project Period (FY) |
2008 – 2011
|
| Keywords | miRNA / タンパク / マイクロアレイ / 神経性食欲不振症 / 長期飢餓ストレス |
|---|
| Research Abstract |
We used 9 week BALB-c mice. We found 40% restriction group was best suited for a model of chronic staving stress model and for further examinations. Microarray analysis using total RNA from mouse hippocampus revealed that expressions of several miRNAs were significantly altered under starvation stress condition compared to control group. In the real-time PCR analysis, miR-7 was significantly up-regulated. One of the putative targets for miR-7 was the mRNA which codes proteins related to neurogenesis and so on. There have been no reports that many miRNA altered under chronic starvation stress. Those findings might contribute to establish a new biomarker of starvation conditions such as anorexia nervosa.
|
