Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using an MCA-microarray and bisulfate pyrosequencing

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590797
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: ITOH Fumio 聖マリアンナ医科大学, 医学部, 教授 (90223180)
• Project Period (FY): 2009 – 2011
• Keywords: 食道癌 / 治療 / 化学療法 / 効果予測 / 網羅的メチル化解析

Research Abstract

Tumor and adjacent normal tissue biopsy specimens were obtained before/after treatment from 34 patients(124 samples) treated with a uniform CRT protocol. We analyzed genomewide DNA methylation of 12 non-treated test samples(T and matched ADJ : 6 each) by methylated CpG island amplification and microarray(MCAM) method, and selected candidate genes that were highly methylated in T compared to ADJ. CYP26C1 was selected by unsupervised hierarchical clustering after MCAM in test samples, and was also ranked in the top 5 of both molecular & cellular function networks as a drug metabolism category. CYP26C1 DNA methylation was significantly higher in T than ADJ in both test and validation samples. CYP26C1 has a CpG island in promoter area, which is highly methylated in T of ESCC patients.

Research Products

• [Journal Article] Prospective study of definitive chemoradiotherapy with S-1 and nedaplatin in patients with stageII/III(non-T4) esophageal cancer (2011)
  • Author(s): Tsuda T, Inaba H, Miyazaki A, Izawa N, Hirakawa M, Watanabe Y, Kitajima S, Hoshikawa Y, Gomi H, Kimura M, Itoh F
  • Journal Title: Esophagus Volume: 8 Pages: 45-51
  • Peer Reviewed
• [Journal Article] Frequent alteration of MLL3 frameshift mutations in microsatellite deficient colorectal cancer (2011)
  • Author(s): Watanabe Y, Castoro RJ, Kim HS, North B, Oikawa R, Hiraishi T, Ahmed SS, Chung W, Cho M-Y, Toyota M, Itoh F, Estecio M R. H., Shen L, Jelinek J, Issa J-P J
  • Journal Title: PLoS ONE Volume: 6(8) Pages: e23320
  • Peer Reviewed
• [Journal Article] HERC2 Interacts with Claspin and regulates DNA origin firing and replication fork progression (2011)
  • Author(s): Izawa N, Wu W, Sato K, Nishikawa H, Kato A, Boku N, Itoh F, Ohta T
  • Journal Title: Cancer Res Volume: 71(17) Pages: 5621-5
  • Peer Reviewed
• [Journal Article] Gastric wash-based molecular testing for antibiotic resistance in helicobacter pylori (2011)
  • Author(s): Baba S, Oishi Y, Watanabe Y, Oikawa R, Morita R, Yoshida Y, Hiraishi T, Maehata T, Nagase Y, Fukuda Y, Nakazawa M, Ishigouoka S, Hattori N, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F
  • Journal Title: Digestion Volume: 84 Pages: 299-305
  • Peer Reviewed
• [Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer
  • Author(s): Oishi Y, Watanabe Y, Yoshida Y, Sato Y, Hiraishi T, Oikawa R, Maehata T, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F
  • Journal Title: Tumour Biol Volume: (in press)
  • Peer Reviewed
• [Presentation] マイクロアレイを用いた消化管がんの網羅的メチル化解析 (2011)
  • Author(s): 渡邊嘉行、伊東文生
  • Organizer: 62th日本電気泳動学会総会シンポジウム1
  • Place of Presentation: 横浜(横浜市開港記念会館)
  • Year and Date: 2011-11-18
• [Presentation] Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using a MCA-microarray and bisulfate pyrosequencing (2011)
  • Author(s): 渡邊嘉行、伊東文生
  • Organizer: 2010 A3フォーサイト事業ミーティング口演
  • Place of Presentation: 札幌(ホテルニューオータニ札幌)
  • Year and Date: 2011-07-09
• [Presentation] Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using a MCA-microarray and bisulfate pyrosequenci (2010)
  • Author(s): 津田享志、渡邊嘉行、伊東文生
  • Organizer: ASCO2010ポスター
  • Place of Presentation: Chicago
  • Year and Date: 2010-06-04